EC Number   |
Reference |
|---|
   2.7.7.B22 | gene APM_2825, unrooted similarity tree of IS1634 transposases and alignment of C-terminal region, overview. Insertion number determination of IS1634-21, IS1634-2, and IS1634-3, DNA sequence determination and analysis, qPCR and inverse PCR expression analysis, determination of inverted repeats and sites |
739660 |
| 2.7.7.B22 | gene EC990741_2310, TN10 includes genes jemA, jemB, jemC, tetR, tetA, tetC, and tetD and the left and right genes IS10L and IS10R, IS10R encodes a functional transposase protein that catalyzes the chemical steps in Tn10/IS10 transposition |
739105 |
| 2.7.7.B22 | genes tnsABCDE, recombinant expression in Escherichia coli strain ER2566 from an pCYB1 intein vector, recombinant expression of TnsC1-85 as MBP-fusion protein in Escherichia coli strain BL21Star(DE3) pET101/DTOPO-TnsD1-309His |
739543 |
| 2.7.7.B22 | His-tagged PAI subdomain of SB transposase (N terminal residues G1-Q53), cloned into pET 21a(+) vector, is expressed in soluble form in Escherichia coli strain BL21-A1 |
739500 |
| 2.7.7.B22 | insertion sequence IS1634, gene Mbar_A1742, Unrooted similarity tree of IS1634 transposases and alignment of C-terminal region, overview |
739660 |
| 2.7.7.B22 | IS608 encoded transposase gene tnpA, DNA sequence determination and analysis |
737917 |
| 2.7.7.B22 | overexpressed in intact form in Escherichia coli |
723834 |
| 2.7.7.B22 | recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) in soluble form, end-point RT-PCR confirms the integrity of AcTPase mRNA during cell culture, RT-PCR expression analysis |
739041 |
| 2.7.7.B22 | recombinant expression of N-terminally thioredoxinA-6xHis-tagged wild-type and mutant enzymes in Escherichia coli strain RosettaII(DE3) |
739149 |
| 2.7.7.B22 | recombinant expression of wild-type and mutant enzymes from plasmid pEGFP-C1, which contains EGFP driven froma CMV promoter, in HEK-293 cells |
738323 |
