Literature summary for 2.7.7.B22 extracted from

Leighton, G.O.; Konnova, T.A.; Idiyatullin, B.; Hurr, S.H.; Zuev, Y.F.; Nesmelova, I.V.
The folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA (2014), PLoS ONE, 9, e112114.

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Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged PAI subdomain of SB transposase (N terminal residues G1-Q53), cloned into pET 21a(+) vector, is expressed in soluble form in Escherichia coli strain BL21-A1	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
soluble His-tagged PAI subdomain of SB transposase from Escherichia coli strain BL21-A1 by nickel affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	The reaction of DNA transposition begins when the transposase enzyme binds to the transposon DNA. Folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA. Only the folded conformation of the specific DNA recognition subdomain of the Sleeping Beauty transposase, the PAI subdomain, binds to the transposon DNA. The PAI subdomain is well folded at low temperatures, but the presence of unfolded conformation gradually increases at temperatures above 15°C. DNA-binding of folded and unfolded conformations of the PAI subdomain, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
sleeping beauty transposase	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	Sleeping Beauty is a member of the mariner family of DNA transposons	Homo sapiens
additional information	temperature dependence of the PAI self-diffusion coefficient at pH 5.0 and pH 7.0 over the temperature range 5-35°C, pH 5.0, detection by NMR spectroscopy and intrinsic tyrosine fluorescence and rayleigh light scattering	Homo sapiens

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Release 2023.1 (January 2023)