2.7.7.B22	-	761944	
2.7.7.B22	cloning of the junctions of the integrated Tgf2 cis-element and the surrounding genomic DNA using inverse PCR. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition, recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain Rossetta 1 (DE3)	739044	
2.7.7.B22	DNA sequence determination and analysis of several IS1341-type transposases (tnpB genes), existence of sense overlapping transcripts (sotRNAs) for all IS1341-type transposases in Halobacterium salinarum strain NRC-1, condition-dependent differential regulation between sotRNAs and their cognate genes. Identification of a UUCA tetraloop motif that is present in other archaea (ncRNA family HgcC) and in a Halobacterium salinarum intergenic ncRNA derived from a palindrome associated transposable elements (PATE). Overexpression of one sotRNA and the PATE-derived RNA harboring the tetraloop motif improves Halobacterium salinarum growth, indicating that these ncRNAs are functional. IS1341-type transposase genes harbor the right end (RE) of the IS element, which may explain the sotRNA motifs found as a retained structure formed at DNA level, also stable at RNA level, i.e. the RE-like tetraloop	739632	
2.7.7.B22	expression in Acidithiobacillus ferrooxidans	760393	
2.7.7.B22	expression in Escherichia coli	760785, 761966, 762411	
2.7.7.B22	expression in HEK-293 and HeLa cells and in mice	762040	
2.7.7.B22	expression in HeLa cell	761863	
2.7.7.B22	expression of wild-type and mutant enzyme Tgf2TPase and transposons in Escherichia coli strain Rosetta1 (DE3)	739693	
2.7.7.B22	full-length Hermes expressed in eukaryotic cells, such as budding yeast and Sf9 cells, similarly forms a large multimeric species consistent with an octamer as judged by gel filtration	738023	
2.7.7.B22	Galileo gene structure comparisons in Drosophila species, overview	738320	
2.7.7.B22	gene APM_2825, unrooted similarity tree of IS1634 transposases and alignment of C-terminal region, overview. Insertion number determination of IS1634-21, IS1634-2, and IS1634-3, DNA sequence determination and analysis, qPCR and inverse PCR expression analysis, determination of inverted repeats and sites	739660	
2.7.7.B22	gene EC990741_2310, TN10 includes genes jemA, jemB, jemC, tetR, tetA, tetC, and tetD and the left and right genes IS10L and IS10R, IS10R encodes a functional transposase protein that catalyzes the chemical steps in Tn10/IS10 transposition	739105	
2.7.7.B22	genes tnsABCDE, recombinant expression in Escherichia coli strain ER2566 from an pCYB1 intein vector, recombinant expression of TnsC1-85 as MBP-fusion protein in Escherichia coli strain BL21Star(DE3) pET101/DTOPO-TnsD1-309His	739543	
2.7.7.B22	His-tagged PAI subdomain of SB transposase (N terminal residues G1-Q53), cloned into pET 21a(+) vector, is expressed in soluble form in Escherichia coli strain BL21-A1	739500	
2.7.7.B22	insertion sequence IS1634, gene Mbar_A1742, Unrooted similarity tree of IS1634 transposases and alignment of C-terminal region, overview	739660	
2.7.7.B22	IS608 encoded transposase gene tnpA, DNA sequence determination and analysis	737917	
2.7.7.B22	overexpressed in intact form in Escherichia coli	723834	
2.7.7.B22	recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) in soluble form, end-point RT-PCR confirms the integrity of AcTPase mRNA during cell culture, RT-PCR expression analysis	739041	
2.7.7.B22	recombinant expression of N-terminally thioredoxinA-6xHis-tagged wild-type and mutant enzymes in Escherichia coli strain RosettaII(DE3)	739149	
2.7.7.B22	recombinant expression of wild-type and mutant enzymes from plasmid pEGFP-C1, which contains EGFP driven froma CMV promoter, in HEK-293 cells	738323