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Literature summary for 2.7.7.B22 extracted from

  • Diaz-Maldonado, H.; Gomez, M.J.; Moreno-Paz, M.; San Martin-Uriz, P.; Amils, R.; Parro, V.; Lopez de Saro, F.J.
    Transposase interaction with the beta sliding clamp: effects on insertion sequence proliferation and transposition rate (2015), Sci. Rep., 5, 13329.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B22

Activating Compound

Activating Compound Comment Organism Structure
additional information increasing the strength of the interaction between beta sliding clamp and transposase results in a higher transposition rate in vivo Methanosarcina barkeri
additional information increasing the strength of the interaction between beta sliding clamp and transposase results in a higher transposition rate in vivo Acidiphilium sp. PM

Cloned(Commentary)

Cloned (Comment) Organism
gene APM_2825, unrooted similarity tree of IS1634 transposases and alignment of C-terminal region, overview. Insertion number determination of IS1634-21, IS1634-2, and IS1634-3, DNA sequence determination and analysis, qPCR and inverse PCR expression analysis, determination of inverted repeats and sites Acidiphilium sp. PM
insertion sequence IS1634, gene Mbar_A1742, Unrooted similarity tree of IS1634 transposases and alignment of C-terminal region, overview Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Acidiphilium sp. PM F7S919
-
-
Methanosarcina barkeri Q465C2
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-
Methanosarcina barkeri DSM 804 Q465C2
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the transposase encoded by Acidiphilium binds to the archaeal sliding clamp (PCNA) of Methanosarcina and to the beta sliding clamp of Acidiphilium, Leptospirillum, and Escherichia coli Acidiphilium sp. PM ?
-
 ?
additional information the transposase encoded by Methanosarcina IS1634 binds to the sliding clamp of Acidiphilium beta Methanosarcina barkeri ?
-
 ?
additional information the transposase encoded by Methanosarcina IS1634 binds to the sliding clamp of Acidiphilium beta Methanosarcina barkeri DSM 804 ?
-
 ?

Synonyms

Synonyms Comment Organism
APM_2825
-
 Acidiphilium sp. PM
IS1634 transposase
-
 Methanosarcina barkeri
IS1634 transposase
-
 Acidiphilium sp. PM
Mbar_A1742
-
 Methanosarcina barkeri

General Information

General Information Comment Organism
evolution IS1634-1 or gene APM_2825 in the sequenced 2007 culture might be a recent insertion in the chromosome Acidiphilium sp. PM
evolution the enzyme is a member of the IS1634 family Methanosarcina barkeri
evolution the enzyme is a member of the IS1634 family Acidiphilium sp. PM
additional information increasing the strength of the interaction between beta sliding clamp and transposase results in a higher transposition rate in vivo. The interaction might determine the potential of insertion sequences to be mobilized in bacterial populations and also their ability to proliferate within chromosomes. Insertion sequences are the simplest mobile genetic elements as they often contain just a single gene encoding the transposase required for transposition Methanosarcina barkeri
additional information increasing the strength of the interaction between beta sliding clamp and transposase results in a higher transposition rate in vivo. The interaction might determine the potential of insertion sequences to be mobilized in bacterial populations and also their ability to proliferate within chromosomes. Insertion sequences are the simplest mobile genetic elements as they often contain just a single gene encoding the transposase required for transposition. Ability of the Acidiphilium IS1634 family member to proliferate in Escherichia coli, an organism in which this IS family has never been detected Acidiphilium sp. PM