Literature summary for 2.7.7.B22 extracted from

Lamb-Palmer, N.; Singh, M.; Dalton, J.; Singh, J.
Prokaryotic expression and purification of soluble maize Ac transposase (2013), Mol. Biotechnol., 54, 685-691.

Search for more literature for 2.7.7.B22

Application

Application	Comment	Organism
molecular biology	the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences and hence are important functional genomics tool for tagging and cloning of unknown gene sequences	Zea mays

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) in soluble form, end-point RT-PCR confirms the integrity of AcTPase mRNA during cell culture, RT-PCR expression analysis	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Zea mays	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90000	-	-	Zea mays

Organism

Organism	UniProt	Comment	Textmining
Zea mays	P08770	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography	Zea mays

Subunits

Subunits	Comment	Organism
?	x * 90000, about, recombinant full-length enzyme, SDS-PAGE	Zea mays
More	AcTPase is an 807aa protein and consists of three N-terminal nuclear localization signals (residues 44-206), a bipartite DNA binding domain (159-206), a catalytic core domain, and a highly conserved C-terminal dimerization domain (674-754). The catalytic core domain of AcTPase is thought to form a retroviral integrase-like fold	Zea mays

Synonyms

Synonyms	Comment	Organism
Ac transposase	-	Zea mays
AcTPase	-	Zea mays
maize activator transposase	-	Zea mays

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the family of hAT transposases	Zea mays
physiological function	the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences, involvement of Ac transposase in Ds movement	Zea mays

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Release 2023.1 (January 2023)