Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes from plasmid pEGFP-C1, which contains EGFP driven froma CMV promoter, in HEK-293 cells | Carassius auratus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of C-terminal deletion mutants, i.e. pEGFP-C1-Tgf2TPDELTA31C containing a 31-aa C-terminal deletion of Tgf2 transposase that included the predicted 15-amino acid NLS, pEGFP-C1-Tgf2TPDELTA120N containing a 120-aa N-terminal deletion of Tgf2 transposase, and pEGFP-C1-Tgf2TPDELTA16C containing a 16-aa C-terminal deletion of Tgf2 transposase. Loss of the nuclear localization signal (NLS) domain results in expression in the cytoplasm but not in the nucleus | Carassius auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | loss of the nuclear localization signal (NLS) domain results in expression in the cytoplasm but not in the nucleus | Carassius auratus | - |
- |
nucleus | 15 aa residues (LLFSPKRARLDTNNF) located from 656 to 670 aa within the C-terminus of Tgf2 transposase can function as a nuclear localization signal (NLS) to assist the transfer of the transposase into the nucleus where it mediates DNA transposition | Carassius auratus | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carassius auratus | H9AER4 | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | amino acid sequence analysis based on Phyre2 prediction suggests that Tgf2 transposase contains an N-terminal zinc finger BED domain (65-120 aa), a helix-turn-helix (HTH) binding structure (163-201 aa) and an RNase-H domain (211-683 aa) with an insertion domain (362-493 aa). The cNLS mapper predicts the presence of a monopartite NLS (656-670 aa) of 15 amino acids (LLFSPKRARLDTNNF) within the RNase-H domain at the C-terminus of Tgf2 transposase. This predicted NLS is located downstream of the DDE residues (D228, D295 and E648). Construction of a 3D model of the NLS and DDE signature of Tgf2 transposase, molecular architecture of full-length Tgf2 transposase overview | Carassius auratus |
Synonyms | Comment | Organism |
---|---|---|
Tgf2 transposase | - |
Carassius auratus |
General Information | Comment | Organism |
---|---|---|
evolution | the goldfish Tgf2 transposon belongs to the Hobo/Activator/Tam3 (hAT) family. hAT elements are an ancient family of transposons and are abundant throughout a variety of species. The NLS of Tgf2 transposase is identical to that of the Tol2 transposase, indicating the evolutionary importance of these sequences for NLS function | Carassius auratus |
malfunction | loss of the nuclear localization signal (NLS) domain results in expression in the cytoplasm but not in the nucleus | Carassius auratus |
physiological function | Tgf2 transposase can mediate efficient gene transfer in teleost fish | Carassius auratus |