The enzyme from maize can use cis-zeatin and UDP-glucose as substrates, but not cis-ribosylzeatin, trans-zeatin or trans-ribosylzeatin. Unlike EC 2.4.1.203, trans-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose cannot act as a donor.
The enzyme from maize can use cis-zeatin and UDPglucose as substrates, but not cis-ribosylzeatin, trans-zeatin or trans-ribosylzeatin. Unlike EC 2.4.1.203, trans-zeatin O-beta-D-glucosyltransferase, UDPxylose cannot act as a donor
The enzyme from maize can use cis-zeatin and UDP-glucose as substrates, but not cis-ribosylzeatin, trans-zeatin or trans-ribosylzeatin. Unlike EC 2.4.1.203, trans-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose cannot act as a donor.
phylogenic relationships between zeatin glycosyltransferases and closely related enzymes of bean, soybean, tomato, maize, rice, and Arabidopsis is studied
wild type and Ubi:ZOG1 maize line overexpressing the ZOG1 gene of Phaseolus lunatus, homozygous and heterozygous plants, overexpression of a trans-zeatin-glucosyltransferase changes the ratios between cis and trans isomers in the Ubi::ZOG1 homozygotes
during early developmental stages of spikelets, the strong hybridization signals are observed in floral meristem (FM) and the primordia of glumes, palea and lemma in out whorls While the stamen primordia are continuing to grow, OscZOG1 expression concentrates on the remaining tissue of the central meristem and developing stamens. At a late stage of spikelet development, the strong hybridization signals are observed in anther locules and filaments
OscZOG1 is highly expressed in primary root meristem and lateral root primordia. Expression patterns of OscZOG1 during lateral root initiation, overview
OscZOG1 is preferentially expressed in shoot and root meristematic tissues and nascent organs. Th strong hybridization signals in the meristematic tissues of inflorescence suggests that OscZOG1 expression is targeted to cells in rapidly growing regions. The early activation of OscZOG1 expression in SAM, leaf primordia and young leaves suggest that OscZOG1 may play a critical role in early seedling growth
transgenic rice lines ectopically overexpressing the cZOGT1 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number
transgenic rice lines ectopically overexpressing the cZOGT2 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number
constitutive expression of OscZOG1 leads to negative effects on the formation of the grain-yielding traits with a marked increase in the accumulation levels of cis-zeatin O-glucoside (cZOG) in the transgenic rice plants. Growth of lateral roots is stimulated in OscZOG1 overexpression lines, but inhibited in OscZOG1 RNA interference lines. Modulation of OscZOG1 expression affects lateral root development in rice. The RNAi transgenic lines exhibit a higher plant height, whereas the plant height of the overexpression lines is reduced with respect to wild-type seedlings when grown in liquid culture conditions in greenhouse for 2 weeks, drastic enhancement of tiller numbers in RNAi transgenic lines, phenotypes, overview
in general, trans-zeatin (tZ) and N6-(DELTA2-isopentenyl)adenine (iP)-types predominate in the spectrum of cytokinins in Brassica napus, with N7-glucosides, namely tZ-N7-glucoside (tZ7G) and iPN7-glucoside (iP7G), representing the most abundant forms. Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves. Cytokinin spectrum and content, overview
the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans
the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans
the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans
cis-zeatin inhibits seminal root elongation and up-regulates cytokinin-inducible genes, and its activities are comparable to those of trans-zeatin. cis-Zeatin activity has a physiological impact on the growth and development of rice
putative zeatin O-glucosyltransferase OscZOG1 regulates root and shoot development and formation of agronomic traits in rice. Cytokinin plays a central role in regulating the activity of the reproductive shoot apical meristem (SAM), which is one parameter determining seed yield in crop plants. OscZOG1 regulates lateral root development. OscZOG1 is highly expressed in shoot meristematic tissues and functions in early seedling growth and tillering. OscZOG1 modulates adult plant height and flag leaf senescence. OscZOG1 regulates panicle development and seed formation
generation of transgenic rice lines by RNA interfering or overexpressing gene OscZOG1, encoding a putative zeatin-O-glucosyltransferase. The RNAi transgenic lines exhibit a higher plant height, whereas the plant height of the overexpression lines is reduced with respect to the wild-type seedlings when grown in liquid culture conditions in greenhouse for 2 weeks, drastic enhancement of tiller numbers in the RNAi transgenic lines. Overexpressing OscZOG1 leads to accumulation of cZOG in transgenic plants
construction of enzyme OscZOG1-overexpression transgenic lines and knockout lines, phenotypes, overview. O-glucosides of cis-zeatin (cZOG) are increased significantly in shoots of the OscZOG1-overexpression transgenic rice line (OE-3) while no significant differences are found in the levels of transzeatin O-glucosides (tZOG) between wild-type and the overexpression transgenic line OE-3. In contrast, the amounts of cZOG are lower in shoots of the OscZOG1-RNAi transgenic line (Ri-1) comapred to the wild-type shoots. Levels of trans-zeatin and cis-zeatin are varied slightly among wild-type, the OscZOG1-RNAi transgenic line and OscZOG1-overexpression transgenic line
recombinant expression of GFP-tagged enzyme in Oryza sativa plants via transfection with the Agrobacterium tumefaciens strain GV3101 system. Quantitative RT-PCR enzyme expression analysis
transgenic Zea mays plants are obtained using an Agrobacterium mediated transformation procedure, Ubi : ZOG1 maize line overexpressing the ZOG1 gene of Phaseolus lunatus display increased levels of zeatin-O-glucoside, zeatin O-glucosylation affects root formation, leaf development, chlorophyll content, senescence, male flower differentiation and the formation of tasselseed
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves
Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition