Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.215 extracted from

  • Kudo, T.; Makita, N.; Kojima, M.; Tokunaga, H.; Sakakibara, H.
    Cytokinin activity of cis-zeatin and phenotypic alterations induced by overexpression of putative cis-zeatin-O-glucosyltransferase in rice (2012), Plant Physiol., 160, 319-331.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.215

Activating Compound

Activating Compound Comment Organism Structure
additional information cZOGTs catalyze O-glucosylation in a ATP-independent manner Oryza sativa Japonica Group

Cloned(Commentary)

Cloned (Comment) Organism
cZOGT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression profiles of cZOGT genes in rice, overview Oryza sativa Japonica Group
cZOGT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression profiles of cZOGT genes in rice, overview Oryza sativa Japonica Group
cZOGT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression profiles of cZOGT genes in rice, overview Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.124
-
 cis-zeatin pH 7.0, 30°C Oryza sativa Japonica Group
0.196
-
 cis-zeatin pH 6.0, 30°C Oryza sativa Japonica Group
0.33
-
 cis-zeatin pH 6.0, 30°C Oryza sativa Japonica Group

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cZOGTs catalyze O-glucosylation in a Mg2+-dependent manner Oryza sativa Japonica Group

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52200
-
 x * 52200, cZOGT3, SDS-PAGE Oryza sativa Japonica Group
55100
-
 x * 55100, cZOGT1, SDS-PAGE Oryza sativa Japonica Group
55400
-
 x * 55400, cZOGT2, SDS-PAGE Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + cis-zeatin Oryza sativa Japonica Group
-
 UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q0JB48 cZOGT3; three isozymes cZOGT1, cZOGT2, and cZOGT3, six nuclear genes cZOG
-
Oryza sativa Japonica Group Q7XU02 cZOGT2; three isozymes cZOGT1, cZOGT2, and cZOGT3, six nuclear genes cZOG
-
Oryza sativa Japonica Group Q7XU03 cZOGT1; three isozymes cZOGT1, cZOGT2, and cZOGT3, six nuclear genes cZOG
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cZOGT1 also shows lower activity with cis-zeatin-riboside, and cZOGT1 also catalyzes O-glucosylation of 2-topolin in vitro Oryza sativa Japonica Group ?
-
 ?
additional information cZOGT2 also shows lower activity with cis-zeatin-riboside, and cZOGT2 also catalyzes O-glucosylation of 2-topolin in vitro Oryza sativa Japonica Group ?
-
 ?
additional information cZOGT3 also shows lower activity with cis-zeatin-riboside Oryza sativa Japonica Group ?
-
 ?
UDP-glucose + cis-zeatin
-
 Oryza sativa Japonica Group UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin cZOGTs catalyze O-glucosylation specifically using UDP-Glc as a Glc donor Oryza sativa Japonica Group UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?

Subunits

Subunits Comment Organism
? x * 52200, cZOGT3, SDS-PAGE Oryza sativa Japonica Group
? x * 55100, cZOGT1, SDS-PAGE Oryza sativa Japonica Group
? x * 55400, cZOGT2, SDS-PAGE Oryza sativa Japonica Group

Synonyms

Synonyms Comment Organism
cZOGT1
-
 Oryza sativa Japonica Group
cZOGT2
-
 Oryza sativa Japonica Group
cZOGT3
-
 Oryza sativa Japonica Group

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryza sativa Japonica Group

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 cis-zeatin pH 7.0, 30°C Oryza sativa Japonica Group
0.53
-
 cis-zeatin pH 6.0, 30°C Oryza sativa Japonica Group
0.9
-
 cis-zeatin pH 6.0, 30°C Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Oryza sativa Japonica Group
7
-
-
 Oryza sativa Japonica Group

General Information

General Information Comment Organism
malfunction cZOGT3 overexpressing lines do not show shortened shoots Oryza sativa Japonica Group
malfunction transgenic rice lines ectopically overexpressing the cZOGT1 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number Oryza sativa Japonica Group
malfunction transgenic rice lines ectopically overexpressing the cZOGT2 gene exhibit short-shoot phenotypes, delay of leaf senescence, and decrease in crown root number Oryza sativa Japonica Group
physiological function cis-zeatin inhibits seminal root elongation and up-regulates cytokinin-inducible genes, and its activities are comparable to those of trans-zeatin. cis-Zeatin activity has a physiological impact on the growth and development of rice Oryza sativa Japonica Group

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 cis-zeatin pH 7.0, 30°C Oryza sativa Japonica Group
1.6
-
 cis-zeatin pH 6.0, 30°C Oryza sativa Japonica Group
4.7
-
 cis-zeatin pH 6.0, 30°C Oryza sativa Japonica Group