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Literature summary for 2.4.1.215 extracted from

  • Trda, L.; Baresova, M.; Sasek, V.; Novakova, M.; Zahajska, L.; Dobrev, P.I.; Motyka, V.; Burketova, L.
    Cytokinin metabolism of pathogenic fungus Leptosphaeria maculans involves isopentenyltransferase, adenosine kinase and cytokinin oxidase/dehydrogenase (2017), Front. Microbiol., 8, 1374 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.215

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + cis-zeatin Leptosphaeria maculans
-
 UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin Brassica napus
-
 UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin Leptosphaeria maculans
-
 UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin Brassica napus
-
 UDP + 9-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin Leptosphaeria maculans v23.1.3
-
 UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin Leptosphaeria maculans v23.1.3
-
 UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin Leptosphaeria maculans JN3
-
 UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin Leptosphaeria maculans JN3
-
 UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-
Leptosphaeria maculans
-
-
-
Leptosphaeria maculans JN3
-
-
-
Leptosphaeria maculans v23.1.3
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Brassica napus
-
leaf
-
 Brassica napus
-
mycelium
-
 Leptosphaeria maculans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + cis-zeatin
-
 Leptosphaeria maculans UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin
-
 Leptosphaeria maculans v23.1.3 UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin
-
 Leptosphaeria maculans JN3 UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin
-
 Brassica napus UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin
-
 Leptosphaeria maculans UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin
-
 Leptosphaeria maculans v23.1.3 UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin
-
 Leptosphaeria maculans JN3 UDP + 7-beta-D-glucosyl-cis-zeatin
-
 ?
UDP-glucose + cis-zeatin
-
 Brassica napus UDP + 9-beta-D-glucosyl-cis-zeatin
-
 ?

Synonyms

Synonyms Comment Organism
cis-zeatin O-glucosyltransferase
-
 Leptosphaeria maculans
cZOG
-
 Leptosphaeria maculans

Expression

Organism Comment Expression
Brassica napus Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves up

General Information

General Information Comment Organism
metabolism in general, trans-zeatin (tZ) and N6-(DELTA2-isopentenyl)adenine (iP)-types predominate in the spectrum of cytokinins in Brassica napus, with N7-glucosides, namely tZ-N7-glucoside (tZ7G) and iPN7-glucoside (iP7G), representing the most abundant forms. Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves. Cytokinin spectrum and content, overview Brassica napus
metabolism the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans Leptosphaeria maculans