BRENDA - Enzyme Database
show all sequences of 2.4.1.215

Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition

Mok, M.C.; Martin, R.C.; Dobrev, P.I.; Vankova, R.; Ho, P.S.; Yonekura-Sakakibara, K.; Sakakibara, H.; Mok, D.W.; Plant Physiol. 137, 1057-1066 (2005)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
cis-zeatin
-
Zea mays
0.18
-
o-topolin
-
Zea mays
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with m-topolin
660252
Zea mays
?
-
-
-
-
UDP-glucose + cis-zeatin
-
660252
Zea mays
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
-
?
UDP-glucose + o-hydroxythidiazuron
128% of the activity with trans-zeatin
660252
Zea mays
UDP + ?
-
-
-
?
UDP-glucose + o-topolin
490% of the activity with cis-zeatin
660252
Zea mays
UDP + O-beta-D-glucosyl-o-topolin
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.01
-
cis-zeatin
-
Zea mays
0.03
-
o-topolin
-
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
cis-zeatin
-
Zea mays
0.18
-
o-topolin
-
Zea mays
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with m-topolin
660252
Zea mays
?
-
-
-
-
UDP-glucose + cis-zeatin
-
660252
Zea mays
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
-
?
UDP-glucose + o-hydroxythidiazuron
128% of the activity with trans-zeatin
660252
Zea mays
UDP + ?
-
-
-
?
UDP-glucose + o-topolin
490% of the activity with cis-zeatin
660252
Zea mays
UDP + O-beta-D-glucosyl-o-topolin
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.01
-
cis-zeatin
-
Zea mays
0.03
-
o-topolin
-
Zea mays
Other publictions for EC 2.4.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736599
Shang
Putative zeatin O-glucosyltran ...
Oryza sativa Japonica Group
J. Integr. Plant Biol.
58
627-641
2015
-
-
1
-
1
-
-
-
2
-
-
1
-
3
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723476
Kudo
Cytokinin activity of cis-zeat ...
Oryza sativa Japonica Group
Plant Physiol.
160
319-331
2012
1
-
1
-
-
-
-
3
-
1
3
3
-
4
-
-
-
-
-
1
-
-
9
1
1
-
-
3
2
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
3
-
3
3
3
-
-
-
-
-
3
-
-
9
3
3
-
-
3
3
-
-
-
-
2
6
-
3
3
688094
Rodo
Over-expression of a zeatin O- ...
Zea mays
J. Exp. Bot.
59
2673-2686
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688632
Meek
-
Isolation of Legume Glycosyltr ...
Zea mays
J. Plant Growth Regul.
27
192-201
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660252
Mok
Topolins and hydroxylated thid ...
Zea mays
Plant Physiol.
137
1057-1066
2005
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
660220
Veach
O-Glucosylation of cis-zeatin ...
Zea mays
Plant Physiol.
131
1374-1380
2003
-
-
-
-
-
-
5
2
-
-
1
-
-
3
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
5
-
2
-
-
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
288693
Martin
A maize cytokinin gene encodin ...
Zea mays
Proc. Natl. Acad. Sci. USA
98
5922-5926
2001
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-