BRENDA - Enzyme Database
show all sequences of 2.4.1.215

Putative zeatin O-glucosyltransferase OscZOG1 regulates root and shoot development and formation of agronomic traits in rice

Shang, X.; Xie, R.; Tian, H.; Wang, Q.; Guo, F.; J. Integr. Plant Biol. 58, 627-641 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene OscZOG1, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme OscZOG1 in Oryza sativa plants using Agrobacterium tumefaciens strain GV3101 transfection
Oryza sativa Japonica Group
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of enzyme OscZOG1-overexpression transgenic lines and knockout lines, phenotypes, overview. O-glucosides of cis-zeatin (cZOG) are increased significantly in shoots of the OscZOG1-overexpression transgenic rice line (OE-3) while no significant differences are found in the levels of transzeatin O-glucosides (tZOG) between wild-type and the overexpression transgenic line OE-3. In contrast, the amounts of cZOG are lower in shoots of the OscZOG1-RNAi transgenic line (Ri-1) comapred to the wild-type shoots. Levels of trans-zeatin and cis-zeatin are varied slightly among wild-type, the OscZOG1-RNAi transgenic line and OscZOG1-overexpression transgenic line
Oryza sativa Japonica Group
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Oryza sativa Japonica Group
5737
-
nucleus
-
Oryza sativa Japonica Group
5634
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + cis-zeatin
Oryza sativa Japonica Group
-
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa Japonica Group
Q7XSZ0
var. Zhonghua 11, gene OscZOG1
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
OscZOG1 is preferentially expressed in shoot and root meristematic tissues and nascent organs
Oryza sativa Japonica Group
-
root
OscZOG1 is highly expressed in primary root meristem and lateral root primordia in rice
Oryza sativa Japonica Group
-
seedling
-
Oryza sativa Japonica Group
-
shoot
-
Oryza sativa Japonica Group
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + cis-zeatin
-
736599
Oryza sativa Japonica Group
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene OscZOG1, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme OscZOG1 in Oryza sativa plants using Agrobacterium tumefaciens strain GV3101 transfection
Oryza sativa Japonica Group
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of enzyme OscZOG1-overexpression transgenic lines and knockout lines, phenotypes, overview. O-glucosides of cis-zeatin (cZOG) are increased significantly in shoots of the OscZOG1-overexpression transgenic rice line (OE-3) while no significant differences are found in the levels of transzeatin O-glucosides (tZOG) between wild-type and the overexpression transgenic line OE-3. In contrast, the amounts of cZOG are lower in shoots of the OscZOG1-RNAi transgenic line (Ri-1) comapred to the wild-type shoots. Levels of trans-zeatin and cis-zeatin are varied slightly among wild-type, the OscZOG1-RNAi transgenic line and OscZOG1-overexpression transgenic line
Oryza sativa Japonica Group
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Oryza sativa Japonica Group
5737
-
nucleus
-
Oryza sativa Japonica Group
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + cis-zeatin
Oryza sativa Japonica Group
-
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
OscZOG1 is preferentially expressed in shoot and root meristematic tissues and nascent organs
Oryza sativa Japonica Group
-
root
OscZOG1 is highly expressed in primary root meristem and lateral root primordia in rice
Oryza sativa Japonica Group
-
seedling
-
Oryza sativa Japonica Group
-
shoot
-
Oryza sativa Japonica Group
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + cis-zeatin
-
736599
Oryza sativa Japonica Group
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
-
?
Other publictions for EC 2.4.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736599
Shang
Putative zeatin O-glucosyltran ...
Oryza sativa Japonica Group
J. Integr. Plant Biol.
58
627-641
2015
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723476
Kudo
Cytokinin activity of cis-zeat ...
Oryza sativa Japonica Group
Plant Physiol.
160
319-331
2012
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6
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688094
Rodo
Over-expression of a zeatin O- ...
Zea mays
J. Exp. Bot.
59
2673-2686
2008
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688632
Meek
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Isolation of Legume Glycosyltr ...
Zea mays
J. Plant Growth Regul.
27
192-201
2008
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660252
Mok
Topolins and hydroxylated thid ...
Zea mays
Plant Physiol.
137
1057-1066
2005
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660220
Veach
O-Glucosylation of cis-zeatin ...
Zea mays
Plant Physiol.
131
1374-1380
2003
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288693
Martin
A maize cytokinin gene encodin ...
Zea mays
Proc. Natl. Acad. Sci. USA
98
5922-5926
2001
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