BRENDA - Enzyme Database
show all sequences of 2.4.1.215

A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-zeatin

Martin, R.C.; Mok, M.C.; Habben, J.E.; Mok, D.W.S.; Proc. Natl. Acad. Sci. USA 98, 5922-5926 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Zea mays
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51100
-
estimated from gene sequence
Zea mays
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
Q93XP7
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cob
lower level of mRNA
Zea mays
-
kernel
lower level of mRNA
Zea mays
-
leaf
very low levels of mRNA
Zea mays
-
root
highest level of mRNA
Zea mays
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates are sugar donors trans-zeatin, dihydrozeatin, respective nucleosides and sugar donors UDP-xylose, UDP-glucuronic acid, UDP-galactose, ADP-glucose, UDP-mannose
288693
Zea mays
?
-
-
-
-
UDP-glucose + cis-zeatin
recombinant protein
288693
Zea mays
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Zea mays
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51100
-
estimated from gene sequence
Zea mays
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Zea mays
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cob
lower level of mRNA
Zea mays
-
kernel
lower level of mRNA
Zea mays
-
leaf
very low levels of mRNA
Zea mays
-
root
highest level of mRNA
Zea mays
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates are sugar donors trans-zeatin, dihydrozeatin, respective nucleosides and sugar donors UDP-xylose, UDP-glucuronic acid, UDP-galactose, ADP-glucose, UDP-mannose
288693
Zea mays
?
-
-
-
-
UDP-glucose + cis-zeatin
recombinant protein
288693
Zea mays
UDP + O-beta-D-glucosyl-cis-zeatin
-
-
-
?
Other publictions for EC 2.4.1.215
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736599
Shang
Putative zeatin O-glucosyltran ...
Oryza sativa Japonica Group
J. Integr. Plant Biol.
58
627-641
2015
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-
1
-
1
-
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-
2
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1
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3
-
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5
-
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1
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1
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1
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2
-
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1
-
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-
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5
-
-
1
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-
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-
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-
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-
723476
Kudo
Cytokinin activity of cis-zeat ...
Oryza sativa Japonica Group
Plant Physiol.
160
319-331
2012
1
-
1
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-
-
-
3
-
1
3
3
-
4
-
-
-
-
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1
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9
1
1
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3
2
-
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3
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3
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3
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3
3
3
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3
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9
3
3
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3
3
-
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-
2
6
-
3
3
688094
Rodo
Over-expression of a zeatin O- ...
Zea mays
J. Exp. Bot.
59
2673-2686
2008
-
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1
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-
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-
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-
-
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4
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3
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1
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1
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3
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1
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-
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-
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-
688632
Meek
-
Isolation of Legume Glycosyltr ...
Zea mays
J. Plant Growth Regul.
27
192-201
2008
-
-
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-
-
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-
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1
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1
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1
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-
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-
660252
Mok
Topolins and hydroxylated thid ...
Zea mays
Plant Physiol.
137
1057-1066
2005
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-
-
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2
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5
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4
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2
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2
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4
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2
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660220
Veach
O-Glucosylation of cis-zeatin ...
Zea mays
Plant Physiol.
131
1374-1380
2003
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-
-
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5
2
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1
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3
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4
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1
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1
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1
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5
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2
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1
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4
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1
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1
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1
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288693
Martin
A maize cytokinin gene encodin ...
Zea mays
Proc. Natl. Acad. Sci. USA
98
5922-5926
2001
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1
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1
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4
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1
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4
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2
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1
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1
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1
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4
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