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1.14.14.80: long-chain fatty acid omega-monooxygenase

This is an abbreviated version!
For detailed information about long-chain fatty acid omega-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.80

Reaction

a long-chain fatty acid
+
[reduced NADPH-hemoprotein reductase]
+
O2
=
an omega-hydroxy-long-chain fatty acid
+
[oxidized NADPH-hemoprotein reductase]
+
H2O

Synonyms

At1g69500, At2g27690, CYP4A, CYP4A1, CYP4A11, CYP4A14, CYP4A2, CYP4A3, CYP4A8, CYP4V2, CYP52-E3, CYP52-M1, CYP52-N1, CYP52E3, CYP52M1, CYP52N1, CYP704B1, CYP86A, CYP86A1, CYP86A33, CYP94C1, cytochrome P450 4A11, cytochrome P450 704B1, cytochrome P450 86A1, EC 1.14.13.205, Fatty acid omega-hydroxylase, Lauric acid omega-hydroxylase, omega-hydroxylase, P450 4A11

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.80 long-chain fatty acid omega-monooxygenase

Cloned

Cloned on EC 1.14.14.80 - long-chain fatty acid omega-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed from pFastbacCYP4V2HIS vector in Escherichia coli DH10Bac. Hexahistidine-tagged CYP4V2 is expressed in Sf-9 cells after infection with the recombinant baculovirus
-
expression in COS-7 cells
expression in Escherichia coli
expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae strain WAT11
expression in yeast WAT11 cells
gene CYP4A11, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli
gene CYP52E3, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strains WAT11 and and INVSc1
gene CYP52M1, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strains WAT11 and and INVSc1. 17-Hydroxy linoleic acid is detected in addition to 18-carboxy linoleic acid (cis-9, cis-12-octadecadien-1,18-dioic acid) in the reaction mixture of linoleic acid with CYP52M1-transformed cells
gene CYP52N1, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strains WAT11 and and INVSc1
gene CYPP4504A11, located on chromosome 7, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time RT PCR enzyme expression analysis, recombinant expression in Escherichia coli