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Literature summary for 1.14.14.80 extracted from

  • Hoch, U.; Zhang, Z.; Kroetz, D.L.; Ortiz de Montellano, P.R.
    Structural determination of the substrate specificities and regioselectivities of the rat and human fatty acid omega-hydroxylases (2000), Arch. Biochem. Biophys., 373, 63-71.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information construction of two complementary chimera between isoforms CYP4A2-CYP4A3 fused at residue 119 (CYP4A2) and 122 (CYP4A3). The chimera in which the first 119 amino acids are from CYP4A2 indicates that the first 120 amino acids control the substrate specificity. The chimera in which the first 122 amino acids are from CYP4A3 is inactive due to a defect in electron transfer to the heme group Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
arachidonic acid pH 7.4, 37°C Rattus norvegicus
0.004
-
myristic acid pH 7.4, 37°C Rattus norvegicus
0.01
-
arachidonic acid pH 7.4, 37°C Homo sapiens
0.013
-
myristic acid pH 7.4, 37°C Rattus norvegicus
0.018
-
oleic acid pH 7.4, 37°C Rattus norvegicus
0.019
-
arachidonic acid pH 7.4, 37°C Rattus norvegicus
0.022
-
lauric acid pH 7.4, 37°C Homo sapiens
0.03
-
lauric acid pH 7.4, 37°C Rattus norvegicus
0.046
-
myristic acid pH 7.4, 37°C Rattus norvegicus
0.064
-
oleic acid pH 7.4, 37°C Rattus norvegicus
0.07
-
myristic acid pH 7.4, 37°C Homo sapiens
0.08
-
lauric acid pH 7.4, 37°C Rattus norvegicus
0.136
-
oleic acid pH 7.4, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q02928
-
-
Rattus norvegicus P08516
-
-
Rattus norvegicus P20816
-
-
Rattus norvegicus P20817
-
-
Rattus norvegicus P24464
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Homo sapiens
-
kidney
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Rattus norvegicus 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 2.4:1 Rattus norvegicus 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 2:1 Rattus norvegicus 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 6:1 Rattus norvegicus 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 2.5:1 Homo sapiens 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 2.5:1 Rattus norvegicus 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 3:1 Rattus norvegicus 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 40:1 Rattus norvegicus 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 6:1 Rattus norvegicus 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 1.2:1 Rattus norvegicus 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 1.6:1 Homo sapiens 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 1.6:1 Rattus norvegicus 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 3:1 Rattus norvegicus 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Rattus norvegicus 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Rattus norvegicus 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 1.6:1 Homo sapiens 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 1.6:1 Rattus norvegicus 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 regioselectivity of omega:omega-1 hydroxylation is 1:1 Rattus norvegicus 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP4A1
-
Rattus norvegicus
CYP4A11
-
Homo sapiens
CYP4A2
-
Rattus norvegicus
CYP4A3
-
Rattus norvegicus
CYP4A8
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
arachidonic acid pH 7.4, 37°C Homo sapiens
0.01
-
oleic acid pH 7.4, 37°C Homo sapiens
0.01
-
oleic acid pH 7.4, 37°C Rattus norvegicus
0.02
-
arachidonic acid pH 7.4, 37°C Rattus norvegicus
0.02
-
oleic acid pH 7.4, 37°C Rattus norvegicus
0.03
-
arachidonic acid pH 7.4, 37°C Rattus norvegicus
0.03
-
palmitic acid pH 7.4, 37°C Rattus norvegicus
0.1
-
arachidonic acid pH 7.4, 37°C Rattus norvegicus
0.1
-
palmitic acid pH 7.4, 37°C Rattus norvegicus
0.1
-
myristic acid pH 7.4, 37°C Rattus norvegicus
0.17
-
palmitic acid pH 7.4, 37°C Homo sapiens
0.58
-
lauric acid pH 7.4, 37°C Rattus norvegicus
0.67
-
myristic acid pH 7.4, 37°C Rattus norvegicus
0.7
-
lauric acid pH 7.4, 37°C Homo sapiens
0.83
-
myristic acid pH 7.4, 37°C Homo sapiens
1
-
palmitic acid pH 7.4, 37°C Rattus norvegicus
1.17
-
myristic acid pH 7.4, 37°C Rattus norvegicus
1.22
-
lauric acid pH 7.4, 37°C Rattus norvegicus
3.8
-
myristic acid pH 7.4, 37°C Rattus norvegicus
3.8
-
lauric acid pH 7.4, 37°C Rattus norvegicus
10.8
-
lauric acid pH 7.4, 37°C Rattus norvegicus