Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.2.10: lanthanide-dependent methanol dehydrogenase

This is an abbreviated version!
For detailed information about lanthanide-dependent methanol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.2.10

Reaction

methanol
+ 2 oxidized cytochrome cL =
formaldehyde
 + 2 reduced cytochrome cL

Synonyms

Ce-MDH, Ce-XoxF, Ce3+-dependent XoxF1-MDH, Ce3+-induced methanol dehydrogenase, cerium dependent MDH, cerium-dependent methanol dehydrogenase, Eu-MDH, exaF, La-XoxF, La2+-MDH-like protein, La3+-dependent MDH, La3+-dependent methanol dehydrogenase, La3+-induced methanol dehydrogenase-like protein, lanthanide-dependent alcohol dehydrogenase, lanthanide-dependent MDH, lanthanide-dependent MeDH, lanthanide-dependent methanol dehydrogenase, lanthanide-dependent quinoid alcohol dehydrogenase, lanthanide-dependent XoxF-type MDH, lanthanide-dependent XoxF-type methanol dehydrogenase, lanthanidedependent XoxF-MDH, lanthanides-dependent MDH, lanthanoid-dependent methanol dehydrogenase, Ln-dependent EtDH, Ln-dependent ethanol dehydrogenase, Ln-dependent MDH, Ln-dependent MetDH, Ln-dependent methanol dehydrogenase, Ln-MDH, methanol dehydrogenase, Nd-XoxF, pyrroloquinoline quinone ethanol dehydrogenase, quinoprotein ethanol dehydrogenase, XoxF, XoxF dehydrogenase, XoxF methanol dehydrogenase, XoxF-MDH, XoxF-type MDH, XoxF-type methanol dehydrogenase, xoxF1, XoxF4-1, XoxF4-2, XoxF5, XoxF5-1, XoxF5-2, XoxF5-type MDH, XoxFMDH, XoxFMeDH, XoxJ

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.2 With a cytochrome as acceptor
                1.1.2.10 lanthanide-dependent methanol dehydrogenase

Purification

Purification on EC 1.1.2.10 - lanthanide-dependent methanol dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Toyopearl butyl-650S column chromatography and HiTrap column chromatography
-
Hi-Trap SP HP Sepharose column chromatography and Mono S column chromatography
-
HiTrap SP Sepharose column chromatography and Mono S column chromatography
-
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA resin column chromatography
-
recombinant enzyme from Methylorubrum extorquens strain AM1 and from Escherichia coli, periplasmic extraction is easier in Escherichia coli than in Methylorubrum extorquens, by ammonium sulfate fractionation, cation exchange and hydrophobic interaction chromatography, and desalting gel filtration. Recombinant XoxJ from Escherichia coli strain BL21(DE3) periplasm by anion exchange chromatography, gel filtration, hydrophobic interaction chromatography, and ultrafiltration, followed by gel filtration, and cleavage of the signal peptide
P16027; P14775; P14774
recombinant wild-type XoxJ from Escherichia coli by anion exchange and hydrophobic interaction chromatography, and gel filtration. Native subunit XoxF is purified by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration
P16027; P14775; P14774
Source 15Q anion exchange column chromatography and Superdex S200 gel filtration
-
SP Sepharose column chromatography and Superdex 75 gel filtration
-
SP-Sepharose column chromatography
-