Literature summary for 1.1.2.10 extracted from

Wang, L.; Suganuma, S.; Hibino, A.; Mitsui, R.; Tani, A.; Matsumoto, T.; Ebihara, A.; Fitriyanto, N.A.; Pertiwiningrum, A.; Shimada, M.; Hayakawa, T.; Nakagawa, T.
Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA110 (2019), Enzyme Microb. Technol., 130, 109371 .

Search for more literature for 1.1.2.10

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.067	-	methanol	at pH 9.0 and 30°C	Bradyrhizobium diazoefficiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ce3+	highest activity with 0.03 mM Ce3+. The enzyme contains 0.58 cerium atoms per subunit	Bradyrhizobium diazoefficiens
La3+	second highest activity with 0.03 mM La3+	Bradyrhizobium diazoefficiens
Nd3+	activates at 0.03 mM	Bradyrhizobium diazoefficiens
Pr3+	activates at 0.03 mM	Bradyrhizobium diazoefficiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
136000	-	calculated from SAXS data	Bradyrhizobium diazoefficiens

Organism

Organism	UniProt	Comment	Textmining
Bradyrhizobium diazoefficiens	-	-	-
Bradyrhizobium diazoefficiens USDA110	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Toyopearl butyl-650S column chromatography and HiTrap column chromatography	Bradyrhizobium diazoefficiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:methanol-grown cell	-	Bradyrhizobium diazoefficiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-butanol + phenazine ethosulfate	78.3% activity compared to methanol	Bradyrhizobium diazoefficiens	butyraldehyde + reduced phenazine ethosulfate	-	r
1-butanol + phenazine ethosulfate	78.3% activity compared to methanol	Bradyrhizobium diazoefficiens USDA110	butyraldehyde + reduced phenazine ethosulfate	-	r
1-propanol + phenazine ethosulfate	85.3% activity compared to methanol	Bradyrhizobium diazoefficiens	propionaldehyde + reduced phenazine ethosulfate	-	r
1-propanol + phenazine ethosulfate	85.3% activity compared to methanol	Bradyrhizobium diazoefficiens USDA110	propionaldehyde + reduced phenazine ethosulfate	-	r
2-propanol + phenazine ethosulfate	0.775% activity compared to methanol	Bradyrhizobium diazoefficiens	?	-	r
2-propanol + phenazine ethosulfate	0.775% activity compared to methanol	Bradyrhizobium diazoefficiens USDA110	?	-	r
ethanol + phenazine ethosulfate	93% activity compared to methanol	Bradyrhizobium diazoefficiens	acetaldehyde + reduced phenazine ethosulfate	-	r
ethanol + phenazine ethosulfate	93% activity compared to methanol	Bradyrhizobium diazoefficiens USDA110	acetaldehyde + reduced phenazine ethosulfate	-	r
formaldehyde + reduced phenazine ethosulfate	91.5% activity compared to methanol	Bradyrhizobium diazoefficiens	methanol + phenazine ethosulfate	-	r
methanol + phenazine ethosulfate	100% activity	Bradyrhizobium diazoefficiens	formaldehyde + reduced phenazine ethosulfate	-	r
methanol + phenazine ethosulfate	100% activity	Bradyrhizobium diazoefficiens USDA110	formaldehyde + reduced phenazine ethosulfate	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 64000, SDS-PAGE	Bradyrhizobium diazoefficiens

Synonyms

Synonyms	Comment	Organism
lanthanides-dependent MDH	-	Bradyrhizobium diazoefficiens
XoxF	-	Bradyrhizobium diazoefficiens
XoxF5-type MDH	-	Bradyrhizobium diazoefficiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Bradyrhizobium diazoefficiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Bradyrhizobium diazoefficiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)