Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 22% (w/v) PEG 8000, 0.2 M NaCl | Methylacidiphilum fumariolicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by cyanide | Methylacidiphilum fumariolicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
methanol | at pH 7.0 and 40-45°C | Methylacidiphilum fumariolicum | |
0.002 | - |
1-Hexanol | at pH 7.0 and 40-45°C | Methylacidiphilum fumariolicum | |
0.003 | - |
ethanol | at pH 7.0 and 40-45°C | Methylacidiphilum fumariolicum | |
0.006 | - |
1-butanol | at pH 7.0 and 40-45°C | Methylacidiphilum fumariolicum | |
0.007 | - |
formaldehyde | at pH 7.0 and 40-45°C | Methylacidiphilum fumariolicum | |
0.007 | - |
1-propanol | at pH 7.0 and 40-45°C | Methylacidiphilum fumariolicum | |
0.2 | - |
acetaldehyde | Km above 0.2 mM, at pH 7.0 and 40-45°C | Methylacidiphilum fumariolicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ce3+ | dependent on | Methylacidiphilum fumariolicum | |
La3+ | dependent on | Methylacidiphilum fumariolicum | |
additional information | the enzyme is completely independent of ammonium | Methylacidiphilum fumariolicum | |
Nd3+ | dependent on | Methylacidiphilum fumariolicum | |
Pr3+ | dependent on | Methylacidiphilum fumariolicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
127000 | - |
gel filtration | Methylacidiphilum fumariolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylacidiphilum fumariolicum | - |
- |
- |
Methylacidiphilum fumariolicum SolV | - |
- |
- |
Purification (Comment) | Organism |
---|---|
SP-Sepharose column chromatography | Methylacidiphilum fumariolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + phenazine ethosulfate | 100% activity compared to methanol | Methylacidiphilum fumariolicum | butyraldehyde + reduced phenazine ethosulfate | - |
? | |
1-butanol + phenazine ethosulfate | 100% activity compared to methanol | Methylacidiphilum fumariolicum SolV | butyraldehyde + reduced phenazine ethosulfate | - |
? | |
1-hexanol + phenazine ethosulfate | 100% activity compared to methanol | Methylacidiphilum fumariolicum | hexaldehyde + reduced phenazine ethosulfate | - |
? | |
1-hexanol + phenazine ethosulfate | 100% activity compared to methanol | Methylacidiphilum fumariolicum SolV | hexaldehyde + reduced phenazine ethosulfate | - |
? | |
1-propanol + phenazine ethosulfate | 100% activity compared to methanol | Methylacidiphilum fumariolicum | propionaldehyde + reduced phenazine ethosulfate | - |
? | |
acetaldehyde + reduced phenazine ethosulfate | less than 50% activity compared to methanol | Methylacidiphilum fumariolicum | ethanol + phenazine ethosulfate | - |
? | |
ethanol + phenazine ethosulfate | 100% activity compared to methanol | Methylacidiphilum fumariolicum | acetaldehyde + reduced phenazine ethosulfate | - |
? | |
formaldehyde + reduced phenazine ethosulfate | 100% activity compared to methanol | Methylacidiphilum fumariolicum | methanol + phenazine ethosulfate | - |
r | |
methanol + phenazine ethosulfate | 100% activity | Methylacidiphilum fumariolicum | formaldehyde + reduced phenazine ethosulfate | - |
r | |
methanol + phenazine ethosulfate | 100% activity | Methylacidiphilum fumariolicum SolV | formaldehyde + reduced phenazine ethosulfate | - |
r | |
methanol + phenazine methosulfate | 100% activity | Methylacidiphilum fumariolicum | formaldehyde + reduced phenazine methosulfate | - |
r | |
methanol + phenazine methosulfate | 100% activity | Methylacidiphilum fumariolicum SolV | formaldehyde + reduced phenazine methosulfate | - |
r | |
additional information | no activity with 2-propanol and formate | Methylacidiphilum fumariolicum | ? | - |
- |
|
additional information | no activity with 2-propanol and formate | Methylacidiphilum fumariolicum SolV | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Methylacidiphilum fumariolicum |
homodimer | 2 * 63502, MALDI-TOF mass spectrometry | Methylacidiphilum fumariolicum |
homodimer | 2 * 63586, calculated from amino acid sequence | Methylacidiphilum fumariolicum |
Synonyms | Comment | Organism |
---|---|---|
lanthanide-dependent MDH | - |
Methylacidiphilum fumariolicum |
Ln-MDH | - |
Methylacidiphilum fumariolicum |
XoxF-type MDH | - |
Methylacidiphilum fumariolicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methylacidiphilum fumariolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methylacidiphilum fumariolicum |