EC Number   |
Reference  |
|---|
   2.7.7.B22 | overexpressed in intact form in Escherichia coli |
723834 |
| 2.7.7.B22 | IS608 encoded transposase gene tnpA, DNA sequence determination and analysis |
737917 |
| 2.7.7.B22 | full-length Hermes expressed in eukaryotic cells, such as budding yeast and Sf9 cells, similarly forms a large multimeric species consistent with an octamer as judged by gel filtration |
738023 |
| 2.7.7.B22 | Galileo gene structure comparisons in Drosophila species, overview |
738320 |
| 2.7.7.B22 | recombinant expression of wild-type and mutant enzymes from plasmid pEGFP-C1, which contains EGFP driven froma CMV promoter, in HEK-293 cells |
738323 |
| 2.7.7.B22 | recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) in soluble form, end-point RT-PCR confirms the integrity of AcTPase mRNA during cell culture, RT-PCR expression analysis |
739041 |
| 2.7.7.B22 | cloning of the junctions of the integrated Tgf2 cis-element and the surrounding genomic DNA using inverse PCR. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition, recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain Rossetta 1 (DE3) |
739044 |
| 2.7.7.B22 | gene EC990741_2310, TN10 includes genes jemA, jemB, jemC, tetR, tetA, tetC, and tetD and the left and right genes IS10L and IS10R, IS10R encodes a functional transposase protein that catalyzes the chemical steps in Tn10/IS10 transposition |
739105 |
| 2.7.7.B22 | recombinant expression of N-terminally thioredoxinA-6xHis-tagged wild-type and mutant enzymes in Escherichia coli strain RosettaII(DE3) |
739149 |
| 2.7.7.B22 | His-tagged PAI subdomain of SB transposase (N terminal residues G1-Q53), cloned into pET 21a(+) vector, is expressed in soluble form in Escherichia coli strain BL21-A1 |
739500 |
