Literature summary for 2.7.7.B22 extracted from

Xu, H.L.; Shen, X.D.; Hou, F.; Cheng, L.D.; Zou, S.M.; Jiang, X.Y.
Prokaryotic expression and purification of soluble goldfish Tgf2 transposase with transposition activity (2015), Mol. Biotechnol., 57, 94-100.

Search for more literature for 2.7.7.B22

Cloned(Commentary)

Cloned (Comment)	Organism
cloning of the junctions of the integrated Tgf2 cis-element and the surrounding genomic DNA using inverse PCR. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition, recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain Rossetta 1 (DE3)	Carassius auratus

Cloned (Comment)

Organism

cloning of the junctions of the integrated Tgf2 cis-element and the surrounding genomic DNA using inverse PCR. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition, recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain Rossetta 1 (DE3)

Carassius auratus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	-	Carassius auratus

Organism

Organism	UniProt	Comment	Textmining
Carassius auratus	H9AER4	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble His6-tagged enzyme from Escherichia coli strain Rossetta 1 (DE3) by nickel affinity chromatography, identification of the recombinant enzyme by MALDI-TOF-TOF tandem MS analysis	Carassius auratus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	for examination of the transposition activity of recombinant Tgf2 TPase, microinjection is performed in blunt snout bream embryos at 1-2 cell stage. When donor plasmid pTgf2-EF1alpha-EGFP is co-injected with recombinant Tgf2 TPase protein, 74% of the embryos show almost ubiquitous and uniform expression of EGFP, while 31% control embryos by injected donor plasmid show only mosaic expression of EGFP. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition	Carassius auratus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 80000, about, recombinant His6-tagged enzyme, SDS-PAGE	Carassius auratus

Synonyms

Synonyms	Comment	Organism
Tgf2 TPase	-	Carassius auratus
Tgf2 transposase	-	Carassius auratus

General Information

General Information	Comment	Organism
evolution	the goldfish Tgf2 transposon belongs to the Hobo/Activator/Tam3 (hAT) family. hAT elements are an ancient family of transposons and are abundant throughout a variety of species	Carassius auratus