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IUBMB CommentsThe substrate is the product of EC 3.5.4.25 GTP cyclohydrolase II.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
riboflavin-specific deaminase,
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Riboflavin-specific deaminase
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RibD
bifunctional enzyme with diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino)uracil reductase activities
RibD
bifunctional enzyme with diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino)uracil reductase activities
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2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
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hydrolysis of amidines
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2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine 2-aminohydrolase
The substrate is the product of EC 3.5.4.25 GTP cyclohydrolase II.
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2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
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8
assay at pH 8
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37
assay at 37°C
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UniProt
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UniProt
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UniProt
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UniProt
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42038
2 * 42038, calculated from amino acid sequence
42441
2 * 42441, MALDI-TOF mass spectrometry
40000
calculation from sequence of cDNA
40000
calculation from sequence of cDNA
40000
2 * 40000, SDS-PAGE
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homodimer
2 * 40000, SDS-PAGE
homodimer
2 * 42038, calculated from amino acid sequence
homodimer
2 * 42441, MALDI-TOF mass spectrometry
homodimer
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2 * 40000, SDS-PAGE
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homodimer
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2 * 42038, calculated from amino acid sequence
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homodimer
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2 * 42441, MALDI-TOF mass spectrometry
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additional information
deaminase and reductase activity
additional information
deaminase and reductase activity
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sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.125 M sodium cacodylate pH 5, 30% (w/v) polyethylene glycol 8000
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-6°C, 50 mM Tris-HCl buffer, pH 8, at least one month
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nickel-charged HisTrap column chromatography and Superdex 200 gel filtration
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expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
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Richter, G.; Fischer, M.; Krieger, C.; Eberhardt, S.; Luttgen, H.; Gerstenschlger, I.; Bacher, A.
Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis
J. Bacteriol.
179
2022-2028
1997
Bacillus subtilis (P17618), Escherichia coli (P25539)
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Burrows, R.B.; Brown, G.M.
Presence in Escherichia coli of a deaminase and a reductase involved in biosynthesis of riboflavin
J. Bacteriol.
136
657-667
1978
Escherichia coli
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Dawson, A.; Trumper, P.; Chrysostomou, G.; Hunter, W.N.
Structure of diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase from Acinetobacter baumannii
Acta Crystallogr. Sect. F
69
611-617
2013
Acinetobacter baumannii (D0CB74), Acinetobacter baumannii ATCC 19606 (D0CB74)
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3.5.4.26 diaminohydroxyphosphoribosylaminopyrimidine deaminase
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