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Literature summary for 3.5.4.26 extracted from

  • Dawson, A.; Trumper, P.; Chrysostomou, G.; Hunter, W.N.
    Structure of diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase from Acinetobacter baumannii (2013), Acta Crystallogr. Sect. F, 69, 611-617.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acinetobacter baumannii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.125 M sodium cacodylate pH 5, 30% (w/v) polyethylene glycol 8000 Acinetobacter baumannii

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Acinetobacter baumannii
42038
-
 2 * 42038, calculated from amino acid sequence Acinetobacter baumannii
42441
-
 2 * 42441, MALDI-TOF mass spectrometry Acinetobacter baumannii
58000
-
 gel filtration Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O Acinetobacter baumannii
-
 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
-
 ?
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O Acinetobacter baumannii ATCC 19606
-
 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii D0CB74
-
-
Acinetobacter baumannii ATCC 19606 D0CB74
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-charged HisTrap column chromatography and Superdex 200 gel filtration Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
-
 Acinetobacter baumannii 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
-
 ?
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
-
 Acinetobacter baumannii ATCC 19606 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Acinetobacter baumannii
homodimer 2 * 42038, calculated from amino acid sequence Acinetobacter baumannii
homodimer 2 * 42441, MALDI-TOF mass spectrometry Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
RibD bifunctional enzyme with diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino)uracil reductase activities Acinetobacter baumannii