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n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
sucrose
alpha-(1,4) glucan
sucrose
alpha-glucan + turanose + trehalulose
sucrose
D-fructose + alpha-1,4-glucan
sucrose
glucose + maltose + maltotriose + soluble maltooligosaccharides + trehalulose + turanose + insoluble glucan
-
-
9.6% glucose + 9.3% maltose + 11.0% maltotriose + 28.8% soluble maltooligosaccharides + 18.4% trehalulose + 15.1% turanose + 7.8% insoluble glucan
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?
sucrose
glucose + maltose + maltotriose + turanose + insoluble polymer
enzyme catalyzes both sucrose hydrolysis and oligosaccharide and polymer synthesis in the absence of an activator polymer
with 10 mM sucrose as the sole substrate, 30% glucose, 29% maltose, 18% maltotriose, 11% turanose and 12% insoluble polymer respectively
?
sucrose
maltose + maltotriose + turanose + erlose
compared to the wild-type enzyme, and in agreement with their loss of polymerase activity, all three mutant enzymes incorporate higher amounts of glucosyl units in maltose (27.3% (mutant enzyme R226L/I228V/F229A/A289I/F290Y/E300I/V331T); 24.8% (mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437R/N439D), 15.3% (mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437S/N439D/C445R), versus 5.8% for wild-type enzyme) and in maltotriose (20.5% (mutant R226L/I228V/F229A/A289I/F290Y/E300I/V331T); 18.6% (mutant R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437R/N439D), 23% (mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437S/N439D/C445R), versus 2.9% for wild-type enzyme). Compared to the others, the mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437S/N439D/C445R is more specialized in turanose production, incorporating nearly 46% of the glucosyl residues in turanose, versus only 19% for the wild-type enzyme. With mutants enzyme R226L/I228V/F229A/A289I/F290Y/E300I/V331T and mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437R/N439D, 20% and 23% glucosyl units are incorporated into erlose (alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructose), respectively. Much lower values are observed with mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437S/N439D/C445R (only 1.4%) and none for the wild-type enzyme. Panose (alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucose) is mainly produced by mutants R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437R/N439D and R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437S/N439D/C445R, 13.9% and 8.5% of the glucosyl units incorporated into this trisaccharide, respectively. In comparison, the value goes down to 1.6% with mutant enzyme R226L/I228V/F229A/A289I/F290Y/E300I/V331T and it is not produced by the wild-type enzyme
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-
?
sucrose + (+)-catechin
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
sucrose + (+)-catechin-3'-O-alpha-D-glucopyranoside
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
sucrose + (+)-taxifolin
D-fructose + (+)-taxifolin-4'-O-alpha-D-glucopyranoside
sucrose + (-)-epicatechin
D-fructose + (-)-epicatechin-3'-O-alpha-D-glucopyranoside
sucrose + (-)-epicatechin-3'-O-alpha-D-glucopyranoside
D-fructose + (-)-epicatechin-3'-O-alpha-D-maltoside
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
sucrose + 4'-hydroxyflavanone
D-fructose + ?
sucrose + 6,7-dihydroxyflavone
D-fructose + ?
sucrose + 6-hydroxyflavanone
D-fructose + ?
sucrose + aesculetin
D-fructose + aesculetin 7-alpha-D-glucopyranoside
-
-
-
?
sucrose + aesculetin 7-alpha-D-glucopyranoside
D-fructose + aesculetin 7-alpha-D-maltoside
-
-
-
?
sucrose + aesculetin 7-alpha-D-maltoside
D-fructose + aesculetin 7-alpha-D-maltotrioside
-
-
-
?
sucrose + aesculin
D-fructose + aesculin 4-alpha-glucoside
-
-
-
?
sucrose + aesculin 4-alpha-glucoside
D-fructose + aesculin 4-alpha-maltoside
sucrose + alpha-D-glucopyranosyl-(1->4)-salicin
D-fructose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-salicin
sucrose + amylopectin
?
waxy corn starch selcted as acceptor. The chain length distribution of the elongated waxy corn starchs indicates that all of the branch chains of waxy corn starch are greatly elongated by amylosucrase before occurrence of starch precipitation. Afterwards, however, amylosucrase merely elongates the branch chains whose non-reducing ends are exposed on the surface of the precipitate
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-
?
sucrose + amylose
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
-
?
sucrose + apigenin
D-fructose + ?
sucrose + arbutin
D-fructose + 4-hydroxyphenyl beta-maltoside
sucrose + arbutin
D-fructose + alpha-D-glucopyranosyl-(1,4)-arbutin
-
i.e. 4-hydroxyphenyl beta-glucopyranoside, a glycosylated hydroquinone, maximum yield of bioconversion of arbutin to arbutin-alpha-glucoside are 83.5% and 43.5% at 35°C in donor to acceptor ratios of 1:0.5 and 1:1, respectively
product identification by TLC and NMR analysis, product structure, overview
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?
sucrose + baicalein
D-fructose + ?
sucrose + baicalein
D-fructose + baicalein 6-O-alpha-D-glucopyranoside
sucrose + carboxy methyl cellulose
D-fructose + ?
-
-
-
-
?
sucrose + catechol
D-fructose + catechol glucoside
sucrose + daidzein diglucoside
D-fructose + daidzein triglucoside
-
-
-
?
sucrose + daidzin
D-fructose + daidzein diglucoside
sucrose + dextran T10
D-fructose + ?
-
-
-
-
?
sucrose + dextran T200
D-fructose + ?
-
-
-
-
?
sucrose + dextran T70
D-fructose + ?
-
-
-
-
?
sucrose + epicatechin-3'-O-alpha-D-maltoside
D-fructose + (-)-epicatechin-3'-O-alpha-D-maltotrioside
sucrose + galactomannan
D-fructose + ?
-
-
-
-
?
sucrose + glycerol
(2S)-1-O-alpha-D-glucosyl-glycerol + (2R)-1-O-alpha-D-glucosyl-glycerol + 2-O-alpha-D-glucosyl-glycerol
sucrose + glycerol
D-fructose + (2R/S)-1-O-alpha-D-glucosyl-glycerol
-
-
-
?
sucrose + glycerol
D-fructose + 2-O-alpha-D-glucosyl-glycerol
-
-
-
?
sucrose + glycogen
D-fructose + ?
-
-
-
-
?
sucrose + homoorientin
D-fructose + ?
sucrose + hydroquinone
D-fructose + alpha-arbutin
sucrose + hydroquinone
D-fructose + hydroquinone alpha-glucopyranoside
sucrose + hydroquinone
D-fructose + hydroquinone alpha-glucoside
sucrose + hydroquinone
D-fructose + hydroquinone-O-alpha-D-glucopyranoside
-
-
-
-
?
sucrose + isoquercetin
D-fructose + isoquercitrin-glucoside
sucrose + isoquercitrin
D-fructose + isoquercitrin glucoside
-
-
-
?
sucrose + isoquercitrin diglucoside
D-fructose + isoquercitrin triglucoside
-
-
-
?
sucrose + isoquercitrin glucoside
D-fructose + isoquercitrin diglucoside
-
-
-
?
sucrose + isoquercitrin-diglucoside
D-fructose + isoquercitrin-triglucoside
sucrose + isoquercitrin-glucoside
D-fructose + isoquercitrin-diglucoside
sucrose + isorhoifolin
D-fructose + isorhoifolin-4'-O-alpha-D-glucopyranoside
sucrose + laminarin
D-fructose + ?
-
-
-
-
?
sucrose + linterised potato starch
D-fructose + ?
-
-
-
-
?
sucrose + luteolin
D-fructose + luteolin-4'-O-alpha-D-glucopyranoside
sucrose + maltobiose
D-fructose + maltotriose
-
-
-
-
?
sucrose + maltoheptaose
?
-
-
-
-
?
sucrose + maltopentaose
D-fructose + maltohexaose
-
-
-
-
?
sucrose + maltopentaose
D-fructose + maltohexaose + maltoheptaose
-
-
-
?
sucrose + maltose
D-fructose + maltotriose
sucrose + maltotetraose
D-fructose + maltopentaose
-
-
-
-
?
sucrose + maltotriose
D-fructose + maltotetraose
-
-
-
-
?
sucrose + phloretin
D-fructose + phloretin glucoside 1 + phloretin glucoside 2 + phloretin glucoside 3
sucrose + phloretin
D-fructose + phloretin glucoside A1 + phloretin glucoside A2 + phloretin glucoside A3
sucrose + phloretin
D-fructose + phloretin-4'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + phloretin-4'-O-alpha-D-glucopyranoside
D-fructose + phloretin-4'-O-alpha-D-maltoside
-
-
-
?
sucrose + phloretin-4'-O-alpha-D-maltoside
D-fructose + phloretin-4'-O-alpha-D-maltotrioside
-
-
-
?
sucrose + phytoglycogen
D-fructose + ?
-
-
-
-
?
sucrose + piceid
D-fructose + glucosyl-alpha-(1->4)-piceid
sucrose + pullulan
D-fructose + ?
-
-
-
-
?
sucrose + quercetin
D-fructose + isoquercitrin
sucrose + resorcinol
D-fructose + resorcinol glucoside
sucrose + salicin
D-fructose + alpha-D-glucopyranosyl-(1,4)-salicin
-
synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule, DGAS specifically synthesizes only one salicin transglycosylation product
product determination by NMR and TLC
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?
sucrose + salicin
D-fructose + alpha-D-glucopyranosyl-(1,4)-salicin + alpha-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,4)-salicin
synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule
i.e. glucosyl salicin and maltosyl salicin, identification by NMR and TLC analysis
-
?
sucrose + salicin
D-fructose + alpha-D-glucopyranosyl-(1->4)-salicin
sucrose + starch
D-fructose + ?
-
-
-
-
?
sucrose + vanillin
D-fructose + vanillin 4-alpha-D-glucopyranoside
-
-
-
?
sucrose + waxy maize amylopectin
D-fructose + ?
-
-
-
-
?
sucrose + waxy maize starch
D-fructose + ?
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-
-
-
?
sucrose + zingerone
D-fructose + zingerone 4-alpha-D-glucopyranoside
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
additional information
?
-
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
84% polymerization products, additionally ASase catalyzes reactions with 10.2% isomerization products and 5.8% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
84% polymerization products, additionally ASase catalyzes reactions with 10.2% isomerization products and 5.8% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
45% polymerization products, additionally ASase catalyzes reactions with 8% isomerization products and 71% hydrolysis products
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?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
45% polymerization products, additionally ASase catalyzes reactions with 8% isomerization products and 71% hydrolysis products
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?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
56.9% polymerization products, additionally ASase catalyzes reactions with 33.5% isomerization products and 9.6% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
56.9% polymerization products, additionally ASase catalyzes reactions with 33.5% isomerization products and 9.6% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
-
82.7% polymerization products, additionally ASase catalyzes reactions with 11.5% isomerization products and 5.8% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
75.5% polymerization products, additionally ASase catalyzes reactions with 15.0% isomerization products and 9.5% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
80.1% polymerization products, additionally ASase catalyzes reactions with 14.5% isomerization products and 5.4% hydrolysis products
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?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
D3A730
additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions
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?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
D3A730
additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
78.4% polymerization products, additionally ASase catalyzes reactions with 19.7% isomerization products and 1.9% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
78.4% polymerization products, additionally ASase catalyzes reactions with 19.7% isomerization products and 1.9% hydrolysis products
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-
?
n sucrose
[(1->4)-alpha-D-glucosyl]n + n fructose
SMQ77851
additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions
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?
sucrose
alpha-(1,4) glucan
when the conversion of 100 mM sucrose is catalyzed at 35°C, 45°C, and 55°C for 24 h, the enzyme produces alpha-(1,4) glucans with average degrees of polymerisation of 59, 45, and 37, respectively
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?
sucrose
alpha-(1,4) glucan
when the conversion of 100 mM sucrose is catalyzed at 35°C, 45°C, and 55°C for 24 h, the enzyme produces alpha-(1,4) glucans with average degrees of polymerisation of 59, 45, and 37, respectively
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?
sucrose
alpha-glucan + turanose + trehalulose
transglucosylation activity is significantly higher than the hydrolytic activity. The main product generated from sucrose is structurally determined to be alpha-(1,4)-glucan. A small amount of glucose is produced by hydrolysis, and sucrose isomers including turanose and trehalulose are generated as minor products. The ratio of hydrolytic, polymerization, and isomerization reactions is calculated to be 5.8:84.0:10.2. The enzyme favors production of long-chain insoluble alpha-glucan at lower temperature
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?
sucrose
alpha-glucan + turanose + trehalulose
transglucosylation activity is significantly higher than the hydrolytic activity. The main product generated from sucrose is structurally determined to be alpha-(1,4)-glucan. A small amount of glucose is produced by hydrolysis, and sucrose isomers including turanose and trehalulose are generated as minor products. The ratio of hydrolytic, polymerization, and isomerization reactions is calculated to be 5.8:84.0:10.2. The enzyme favors production of long-chain insoluble alpha-glucan at lower temperature
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-
?
sucrose
D-fructose + alpha-1,4-glucan
WP_018466847
alpha-1,4-glucan is the predominant product at pH 6.0-8.0 and 30-60°C
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-
?
sucrose
D-fructose + alpha-1,4-glucan
WP_018466847
alpha-1,4-glucan is the predominant product at pH 6.0-8.0 and 30-60°C
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-
?
sucrose
turanose + ?
D3A730
the reaction pattern of the enzyme at a sucrose range of 0.1-1.0 M shows that at 0.7 M of sucrose, the production yield of insoluble linearx02alpha-(1,4)-glucans reaches 24% maximum, and any further increase in sucrose results in a slight decrease in yield. The production yield of turanose significantly increases from 16 to 29% by increasing sucrose from 0.1 to 1.0 M. The synthesized glucan has degrees of polymerization for 0.1, 0.4, 0.7,and 1.0 M sucrose, the degree of polymerization values are 77, 49, 39, and 31 respectively
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-
?
sucrose
turanose + ?
D3A730
the reaction pattern of the enzyme at a sucrose range of 0.1-1.0 M shows that at 0.7 M of sucrose, the production yield of insoluble linearx02alpha-(1,4)-glucans reaches 24% maximum, and any further increase in sucrose results in a slight decrease in yield. The production yield of turanose significantly increases from 16 to 29% by increasing sucrose from 0.1 to 1.0 M. The synthesized glucan has degrees of polymerization for 0.1, 0.4, 0.7,and 1.0 M sucrose, the degree of polymerization values are 77, 49, 39, and 31 respectively
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?
sucrose + (+)-catechin
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
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-
-
?
sucrose + (+)-catechin
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
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-
-
?
sucrose + (+)-catechin
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
-
?
sucrose + (+)-catechin-3'-O-alpha-D-glucopyranoside
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
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-
-
?
sucrose + (+)-catechin-3'-O-alpha-D-glucopyranoside
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
sucrose + (+)-taxifolin
D-fructose + (+)-taxifolin-4'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + (+)-taxifolin
D-fructose + (+)-taxifolin-4'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + (-)-epicatechin
D-fructose + (-)-epicatechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + (-)-epicatechin
D-fructose + (-)-epicatechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + (-)-epicatechin-3'-O-alpha-D-glucopyranoside
D-fructose + (-)-epicatechin-3'-O-alpha-D-maltoside
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-
-
?
sucrose + (-)-epicatechin-3'-O-alpha-D-glucopyranoside
D-fructose + (-)-epicatechin-3'-O-alpha-D-maltoside
-
-
-
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
absolute requirement for primer
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
needs glucan from Neisseria sp. as primer molecule
highly branched
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
needs glucan from Neisseria sp. as primer molecule
highly branched
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
needs glucan from Neisseria sp. as primer molecule
highly branched
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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alpha-D-galactopyranosyl-beta-D-fructofuranoside, i.e. galsucrose can replace sucrose, no activity with beta-D-fructofuranosyl-alpha-D-xyloside, i.e. xylsucrose, melibiose and raffinose
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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transfers glucose to growing alpha-1,4-glucan chains
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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alpha-D-glucopyranosyl fluoride can replace sucrose
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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no activity with 3-deoxysucrose and alpha-D-allopyranosyl beta-fructofuranoside
glycogen-like polysaccharide
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
needs mussel or sweet corn glycogen, or corn amylopectin as primer molecule, sucrose alone is no substrate, beta-D-galactosylsucrose can replace sucrose
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
needs glucan from Neisseria sp. as primer molecule
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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needs glucan from Neisseria sp. as primer molecule
highly branched
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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no activity with melezitose
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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no activity with melezitose
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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involved in biosynthesis of amylopectin-glycogen type polysaccharide
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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transfers glucose to growing alpha-1,4-glucan chains
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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constitutive enzyme
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?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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recombinant enzyme linearly elongates some branched chains of glycogen to an average degree of polymerization of 75
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
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-
recombinant enzyme produces glucopolysaccharide mainly composed of alpha-(1-4) glucosidic linkages and a very low degree, i.e. less than 5%, of alpha-(1-6) branched linkages
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
amylosucrase initializes polymer formation by releasing, through sucrose hydrolysis, a glucose molecule that is subsequently used as the first acceptor molecule. Maltooligosaccharides of increasing size are produced and successively elongated at their nonreducing ends until they reached a critical size and concentration, causing precipitation
-
-
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
glycogen is the best D-glucosyl unit acceptor. Semiprocessive glycogen elongation mechanism can be proposed on the basis of modeling data
-
-
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
needs glucan from Neisseria sp. as primer molecule
highly branched
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
constitutive enzyme
-
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
needs glucan from Neisseria sp. as primer molecule
highly branched
?
sucrose + (1,4-alpha-D-glucosyl)n
D-fructose + (1,4-alpha-D-glucosyl)n+1
-
constitutive enzyme
-
?
sucrose + 4'-hydroxyflavanone
D-fructose + ?
3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and mono-hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules
-
-
?
sucrose + 4'-hydroxyflavanone
D-fructose + ?
3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and mono-hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules
-
-
?
sucrose + 6,7-dihydroxyflavone
D-fructose + ?
56% conversion
-
-
?
sucrose + 6,7-dihydroxyflavone
D-fructose + ?
56% conversion
-
-
?
sucrose + 6-hydroxyflavanone
D-fructose + ?
3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and -hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules
-
-
?
sucrose + 6-hydroxyflavanone
D-fructose + ?
3-OH and 7-OH positions of the mono-hydroxyflavones and -hydroxyflavanones are resistant to transglycosylation by Deinococcus geothermalis amylosucrase, whereas the 6-OH and 4'-OH positions of the mono-hydroxyflavones and -hydroxyflavanones exhibit relatively strong transglycosylation reactivities with the glucose donors released from sucrose by amylosucrase from Deinococcus geothermalis. The 6-OH position is considerably more reactive (54fold higher kcat) than the 4'-OH position in both hydroxyflavones and hydroxyflavanones. Further, the transglycosylation reactions with di- and tri-hydroxyflavones and hydroxyflavanones are also investigated and observed to exhibit similar results to those observed for the mono-hydroxyflavones and mono-hydroxyflavanones molecules
-
-
?
sucrose + aesculin 4-alpha-glucoside
D-fructose + aesculin 4-alpha-maltoside
-
-
-
?
sucrose + aesculin 4-alpha-glucoside
D-fructose + aesculin 4-alpha-maltoside
-
-
-
?
sucrose + alpha-D-glucopyranosyl-(1->4)-salicin
D-fructose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-salicin
-
-
-
?
sucrose + alpha-D-glucopyranosyl-(1->4)-salicin
D-fructose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-salicin
-
-
-
?
sucrose + alpha-D-glucopyranosyl-(1->4)-salicin
D-fructose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-salicin
-
-
-
?
sucrose + apigenin
D-fructose + ?
19.6% conversion
-
-
?
sucrose + apigenin
D-fructose + ?
19.6% conversion
-
-
?
sucrose + arbutin
D-fructose + 4-hydroxyphenyl beta-maltoside
-
-
-
?
sucrose + arbutin
D-fructose + 4-hydroxyphenyl beta-maltoside
-
-
-
?
sucrose + baicalein
D-fructose + ?
59% conversion
-
-
?
sucrose + baicalein
D-fructose + ?
59% conversion
-
-
?
sucrose + baicalein
D-fructose + baicalein 6-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + baicalein
D-fructose + baicalein 6-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + catechol
D-fructose + catechol glucoside
-
-
-
?
sucrose + catechol
D-fructose + catechol glucoside
-
-
-
?
sucrose + daidzin
D-fructose + daidzein diglucoside
-
-
-
?
sucrose + daidzin
D-fructose + daidzein diglucoside
-
-
-
?
sucrose + epicatechin-3'-O-alpha-D-maltoside
D-fructose + (-)-epicatechin-3'-O-alpha-D-maltotrioside
-
-
-
?
sucrose + epicatechin-3'-O-alpha-D-maltoside
D-fructose + (-)-epicatechin-3'-O-alpha-D-maltotrioside
-
-
-
?
sucrose + glycerol
(2S)-1-O-alpha-D-glucosyl-glycerol + (2R)-1-O-alpha-D-glucosyl-glycerol + 2-O-alpha-D-glucosyl-glycerol
-
glycerol is transglycosylated by the intermolecular transglycosylation activity of MFAS. The two major products are determined to be (2S)-1-O-alpha-D-glucosyl-glycerol or (2R)-1-O-alpha-D-glucosyl-glycerol, and 2-O-alpha-D-glucosyl-glycerol, in which a glucose molecule is linked to glycerol via an alpha-glycosidic linkage, NMR identification
-
-
?
sucrose + glycerol
(2S)-1-O-alpha-D-glucosyl-glycerol + (2R)-1-O-alpha-D-glucosyl-glycerol + 2-O-alpha-D-glucosyl-glycerol
-
glycerol is transglycosylated by the intermolecular transglycosylation activity of MFAS. The two major products are determined to be (2S)-1-O-alpha-D-glucosyl-glycerol or (2R)-1-O-alpha-D-glucosyl-glycerol, and 2-O-alpha-D-glucosyl-glycerol, in which a glucose molecule is linked to glycerol via an alpha-glycosidic linkage, NMR identification
-
-
?
sucrose + glycogen
?
-
-
-
-
?
sucrose + glycogen
?
-
-
-
-
?
sucrose + homoorientin
D-fructose + ?
57.0% conversion
-
-
?
sucrose + homoorientin
D-fructose + ?
57.0% conversion
-
-
?
sucrose + hydroquinone
D-fructose + alpha-arbutin
-
-
-
?
sucrose + hydroquinone
D-fructose + alpha-arbutin
-
-
-
?
sucrose + hydroquinone
D-fructose + hydroquinone alpha-glucopyranoside
-
-
-
?
sucrose + hydroquinone
D-fructose + hydroquinone alpha-glucopyranoside
-
-
-
?
sucrose + hydroquinone
D-fructose + hydroquinone alpha-glucopyranoside
-
-
-
?
sucrose + hydroquinone
D-fructose + hydroquinone alpha-glucoside
-
-
-
?
sucrose + hydroquinone
D-fructose + hydroquinone alpha-glucoside
-
-
-
?
sucrose + isoquercetin
D-fructose + isoquercitrin-glucoside
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + isoquercetin
D-fructose + isoquercitrin-glucoside
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + isoquercitrin-diglucoside
D-fructose + isoquercitrin-triglucoside
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + isoquercitrin-diglucoside
D-fructose + isoquercitrin-triglucoside
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + isoquercitrin-glucoside
D-fructose + isoquercitrin-diglucoside
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + isoquercitrin-glucoside
D-fructose + isoquercitrin-diglucoside
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + isorhoifolin
D-fructose + isorhoifolin-4'-O-alpha-D-glucopyranoside
1.8% conversion
-
-
?
sucrose + isorhoifolin
D-fructose + isorhoifolin-4'-O-alpha-D-glucopyranoside
1.8% conversion
-
-
?
sucrose + luteolin
D-fructose + luteolin-4'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + luteolin
D-fructose + luteolin-4'-O-alpha-D-glucopyranoside
86.0% conversion
-
-
?
sucrose + luteolin
D-fructose + luteolin-4'-O-alpha-D-glucopyranoside
-
-
-
?
sucrose + maltose
D-fructose + maltotriose
the enzyme also produces (+)-catechin maltooligosaccharides
-
-
?
sucrose + maltose
D-fructose + maltotriose
the enzyme also produces (+)-catechin maltooligosaccharides
-
-
?
sucrose + maltose
D-fructose + maltotriose
-
-
-
-
?
sucrose + phloretin
D-fructose + phloretin glucoside 1 + phloretin glucoside 2 + phloretin glucoside 3
the enzyme catalyzes the stereospecific glucosylation of phloretin at the 4'-position
-
-
?
sucrose + phloretin
D-fructose + phloretin glucoside 1 + phloretin glucoside 2 + phloretin glucoside 3
the enzyme catalyzes the stereospecific glucosylation of phloretin at the 4'-position
-
-
?
sucrose + phloretin
D-fructose + phloretin glucoside A1 + phloretin glucoside A2 + phloretin glucoside A3
the enzyme is a non-Leloir glycosyltransferase that catalyzes the stereospecific glucosylation of phloretin at the 4'-position. Phloretin and its glucosylation derivatives are cytotoic, overview
three major phloretin-dependent sugar-positive products are observed containing one to three Glc residues (Phlo-A1, -A2, -A3), identification by TLC and NMR spectrometry. In all three metabolites the first Glc, GlcA, is linked to the aglycone at C4'
-
?
sucrose + phloretin
D-fructose + phloretin glucoside A1 + phloretin glucoside A2 + phloretin glucoside A3
the enzyme is a non-Leloir glycosyltransferase that catalyzes the stereospecific glucosylation of phloretin at the 4'-position. Phloretin and its glucosylation derivatives are cytotoic, overview
three major phloretin-dependent sugar-positive products are observed containing one to three Glc residues (Phlo-A1, -A2, -A3), identification by TLC and NMR spectrometry. In all three metabolites the first Glc, GlcA, is linked to the aglycone at C4'
-
?
sucrose + piceid
D-fructose + glucosyl-alpha-(1->4)-piceid
-
-
-
?
sucrose + piceid
D-fructose + glucosyl-alpha-(1->4)-piceid
-
-
-
?
sucrose + quercetin
D-fructose + isoquercitrin
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + quercetin
D-fructose + isoquercitrin
isoquercitrin i.e. quercetin-3-O-beta-D-glucoside
-
-
?
sucrose + resorcinol
D-fructose + resorcinol glucoside
-
-
-
?
sucrose + resorcinol
D-fructose + resorcinol glucoside
-
-
-
?
sucrose + salicin
D-fructose + alpha-D-glucopyranosyl-(1->4)-salicin
-
-
-
?
sucrose + salicin
D-fructose + alpha-D-glucopyranosyl-(1->4)-salicin
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
amylosucrases catalyze the formation of an alpha-1,4-glucosidic linkage by transferring a glucosyl unit from sucrose onto an acceptor alpha-1,4-glucan
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
the enzyme shows polymerization activity using sucrose as a sole substrate
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
the enzyme shows polymerization activity using sucrose as a sole substrate
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
linear alpha-(1,4)-glucans
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
the enzyme catalyzes the synthesis of alpha-1,4 glucans from sucrose. The product profile is quite polydisperse, ranging from soluble chains called maltooligosaccharides to high-molecular weight insoluble amylose
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/Q437S/N439D/C445A only produces soluble oligosaccharides as no insoluble high molecular weight amylose is observed
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
-
?
sucrose + [(1->4)-alpha-D-glucosyl]n
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
additional information
?
-
-
amylosucrase is a transglucosidase that catalyses the synthesis of an amylose-type polymer from sucrose, an abundant agro-resource
-
-
?
additional information
?
-
-
the purified recombinant enzyme produces an alpha-glucan at 50°C, with an average degree of polymerization of 45 and a polymerization yield of 76%
-
-
?
additional information
?
-
-
the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose
-
-
?
additional information
?
-
-
arbutin-alpha-glucoside exhibits inhibitory activities of on mushroom tyrosinase and the melanin production in human melanoma cells
-
-
?
additional information
?
-
-
the hydrolysis reaction is not a rate-limiting step to perform transglycosylation in rDGAS
-
-
?
additional information
?
-
-
enzymatic production of trehalose from sucrose using amylosucrase and maltooligosyltrehalose synthase-trehalohydrolase, overview
-
-
?
additional information
?
-
NMR product analysis, overview
-
-
?
additional information
?
-
-
NMR product analysis, overview
-
-
?
additional information
?
-
-
synthesis of sucrose isomers turanose and trehalulose from sucrose in the presence of fructose by DgAS, turanose binding site structure, overview
-
-
?
additional information
?
-
amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
-
-
?
additional information
?
-
-
amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
-
-
?
additional information
?
-
luteolin transglucosylation activity in Corynebacterium glutamicum amylosucrase (cDGAS) is 10% higher than that in the Corynebacterium glutamicum enzyme expressed in Escherichia coli (eDGAS)
-
-
-
additional information
?
-
-
luteolin transglucosylation activity in Corynebacterium glutamicum amylosucrase (cDGAS) is 10% higher than that in the Corynebacterium glutamicum enzyme expressed in Escherichia coli (eDGAS)
-
-
-
additional information
?
-
luteolin transglucosylation activity in Corynebacterium glutamicum amylosucrase (cDGAS) is 10% higher than that in the Corynebacterium glutamicum enzyme expressed in Escherichia coli (eDGAS)
-
-
-
additional information
?
-
NMR product analysis, overview
-
-
?
additional information
?
-
-
NMR product analysis, overview
-
-
?
additional information
?
-
-
amylosucrase is a transglucosidase that catalyzes amylose-like polymer synthesis from sucrose substrate
-
-
?
additional information
?
-
-
amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate
-
-
?
additional information
?
-
-
the enzyme does not require a nucleotide-activated sugar as a glucosyl-donor
-
-
?
additional information
?
-
-
amylosucrase is a versatile enzyme that carries out 3 different catalytic reactions: 1. hydrolysis of sucrose to release a glucose molecule and a fructose molecule, 2. synthesis of glucose polymers from liberated glucose molecules, and 3. production of the sucrose isomers turanose and isomaltulose through an isomerization reaction. In addition, the enzyme can attach glucose molecules to an atypical substrate, thereby generating unnatural glucan-conjugates. The enzyme produces glucose, fructose, soluble maltooligosaccharide, insoluble glucan, and sucrose isomers (turanose and trehalulose) using only sucrose as a substrate
-
-
?
additional information
?
-
-
the enzyme does not require a nucleotide-activated sugar as a glucosyl-donor
-
-
?
additional information
?
-
-
the purified recombinant enzyme displays a typical amylosucrase activity by the demonstration of multiple activities of hydrolysis, isomerization, and polymerization. The enzyme also shows sucrose hydrolysis activity
-
-
?
additional information
?
-
-
the purified recombinant enzyme displays a typical amylosucrase activity by the demonstration of multiple activities of hydrolysis, isomerization, and polymerization. The enzyme also shows sucrose hydrolysis activity
-
-
?
additional information
?
-
-
the recombinant amylosucrase is used to glucosylate glycogen particles in vitro in the presence of sucrose as the glucosyl donor. The morphology and structure of the resulting insoluble products are shown to strongly depend on the initial sucrose/glycogen weight ratio. For the lower ratio (1.14), all glucose molecules produced from sucrose are transferred onto glycogen, yielding a slight elongation of the external chains and their organization into small crystallites at the surface of the glycogen particles. With a high initial sucrose/glycogen ratio (342), the external glycogen chains are extended by amylosucrase, yielding dendritic nanoparticles with a diameter 4-5 times that of the initial particle
-
-
?
additional information
?
-
the enzyme catalyzes the synthesis of a water-insoluble amylose-like polymer from sucrose, a readily available and low-cost agroresource
-
-
?
additional information
?
-
the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose
-
-
?
additional information
?
-
-
the enzyme catalyze the synthesis of an alpha-(1,4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose
-
-
?
additional information
?
-
product patterns formed by wild-type enzyme and selected genetic variants in the presence of sucrose as the sole substrate, overview
-
-
?
additional information
?
-
-
synthesis of cycloamyloses from sucrose by dual enzyme treatment via combined reaction of amylosucrase and 4-alpha-glucanotransferase from Synechocystis sp., EC 2.4.1.25, overview
-
-
?
additional information
?
-
synthesis of sucrose isomers turanose and trehalulose from sucrose in the presence of fructose by NpAS, turanose binding site structure, overview
-
-
?
additional information
?
-
-
synthesis of sucrose isomers turanose and trehalulose from sucrose in the presence of fructose by NpAS, turanose binding site structure, overview
-
-
?
additional information
?
-
amylosucrase (AS), a glucosyltransferase from Neiserria polysaccharea, produces an insoluble alpha-1,4-linked glucan polymer by consuming sucrose and releasing fructose. This reaction does not require a-D-glucosyl-nucleotide-diphosphate like ADP- or UDP-glucose, but rather uses the energy generated by splitting sucrose in order to synthesise the glucan polymer
-
-
?
additional information
?
-
-
amylosucrase from Neisseria polysaccharea is a transglucosylase that synthesizes an insoluble amylose-like polymer from sole substrate sucrose, product isolation and analysis, overview
-
-
?
additional information
?
-
amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
-
-
?
additional information
?
-
-
amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate
-
-
?
additional information
?
-
the amylosucrase from Neisseria polysaccharea naturally catalyzes the synthesis of alpha-glucans from the widely available donor sucrose. NpAS is highly specific for its natural substrate and subsite -1 (according to GH nomenclature) plays a major role in the recognition of the sucrose glucosyl moiety through a highly efficient hydrogen bonding interaction network, subsite 21 is responsible for the high affinity for sucrose
-
-
?
additional information
?
-
-
the amylosucrase from Neisseria polysaccharea naturally catalyzes the synthesis of alpha-glucans from the widely available donor sucrose. NpAS is highly specific for its natural substrate and subsite -1 (according to GH nomenclature) plays a major role in the recognition of the sucrose glucosyl moiety through a highly efficient hydrogen bonding interaction network, subsite 21 is responsible for the high affinity for sucrose
-
-
?
additional information
?
-
-
treatment of pre-gelatinized rice and barley starches with amylosucrase from Neisseria polysaccharea for resistant starch production. Analysis of reaction efficiency, resistant starch content, amylopectin branch-chain length distribution, solubility, welling power, pasting viscosity, and thermal transition properties, detailed overview
-
-
?
additional information
?
-
analysis of enzyme substrate specificity, from 11 potential donors harboring selective derivatizations that are experimentally evaluated, only 4-nitrophenyl-alpha-D-glucopyranoside is used by the wild-type enzyme, and this underlines the high specificity of the -1 subsite of enzyme NpAS for glucosyl donor substrates. Acceptor substrate promiscuity is explored by screening 20 hydroxylated molecules, including D- and L-monosaccharides as well as polyols. With the exception of one compound, all are successfully glucosylated, and showig the tremendous plasticity of the +1 subsite of NpAS, which is responsible for acceptor recognition. Acceptor substrates are arabinose, galactose, altrose, fucose, xylose, allose, mannose, D-sorbitol, Darabitol, D-mannitol, xylitol, myo-inositol, and maltitol. Analysis of product structures and enzyme enantiopreference by in silico docking analyses. The enzyme is able to discriminate very similar molecules such as enantiomers. Arabinose and altrose are more efficiently glucosylated by NpAS in their L forms, whereas xylose is better recognized in its D form. Glucosylation of mannose, xylose, and galactose are less discriminant, while the enzyme isstrictly enantiospecific toward D-fucose
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additional information
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crystalline structures of waxy corn starch treated with the enzyme, detailed overview. The crystalline structures in the amylosucrase-modified starch are the result of the formation of intermolecular double helices among amylopectins with elongated external chains. The degree of mutual binding by hydrogen bonds between amylopectins is responsible for the amount of crystalline structure. When these bonds are strong and numerous, the chains associate as crystalline structures, resulting in high SDS and/or RS content. The internal structures of AS-modified starch are not significantly different from the control. This is a plausible explanation for the insignificant change in RS content of the AS-modified starches with the varying reaction times
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additional information
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hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside is used for activity measurements. Substrate specificities of recombinant wild-type and mutant enzymes, overview
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additional information
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hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside is used for activity measurements. Substrate specificities of recombinant wild-type and mutant enzymes, overview
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additional information
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in presence of an activator polymer , in vitro, the enzyme is capable to caalyze the synthesis of an amylose-like polysaccharide composed of only alpha-1,4-linkages using sucrose as the only energy source
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additional information
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the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose
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additional information
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the enzyme AMS exhibits multiple catalytic activities. Primarily, it can hydrolyze sucrose to glucose and fructose or transfer glucose from sucrose hydrolysis to another glucose or acceptormolecule. As a side reaction, it is also able to catalyze the isomerization of sucrose to turanose or trehalulose
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biotechnology
the enzyme fused to a starch-binding domain (SBD) is introduced in two potato genetic backgrounds to synthesize starch granules with altered composition, and thereby to broaden starch applications. The modified larger starches not only have great benefit to the potato starch industry by reducing losses during starch isolation, but also have an advantage in many food applications such as frozen food due to its extremely high freeze-thaw stability. Modified starches show a higher digestibility after alpha-amylase treatment
biotechnology
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treatment of pre-gelatinized rice and barley starches with amylosucrase from Neisseria polysaccharea is a potential way of replacing commercial resistant starch production
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
D3A730
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
biotechnology
arbutin as a safe hydroquinone derivative is one of most important skin-whitening ingredients including beta-arbutin and alpha-arbutin. The batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
arbutin as a safe hydroquinone derivative is one of most important skin-whitening ingredients including beta-arbutin and alpha-arbutin. The batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
biotechnology
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
D3A730
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
drug development
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
D3A730
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
food industry
cyclodextrins are frequently utilized chemical substances in the food, pharmaceutical, cosmetics, and chemical industries. An enzymatic process for cyclodextrin production is developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase from Paenibacillus macerans is applied to produce the cyclodextrins from linear alpha-(1,4)-glucans, which are obtained by Neisseria polysaccharea amylosucrase treatment on sucrose. The greatest cyclodextrin yield (21.1%, w/w) is achieved from a one-pot dual enzyme reaction at 40°C for 24 h. The maximum level of cyclodextrin production (15.1 mg/ml) is achieved with 0.5 M sucrose in a simultaneous mode of dual enzyme reaction, whereas the reaction with 0.1 M sucrose is the most efficient with regard to conversion yield. Dual enzyme synthesis of cyclodextrins is successfully carried out with no need of starch material. Efficient bioconversion process that does not require the high temperature necessary for starch liquefaction by thermostable alpha-amylase in conventional industrial processing
food industry
D3A730
the enzyme be a promising candidate for food industrial production of linear alpha-(1,4)-glucans and turanose as a next generation sweetener
food industry
the study investigates the differences in structural and physicochemical properties, especially contents of resistant starch, between native and acid-thinned waxy corn starches treated with amylosucrase from Neisseria polysaccharea. The enzyme exhibits similar catalytic efficiency for both forms of starch. The modified starches have higher proportions of long (DP > 33) and intermediate chains (DP 13-33), and X-ray diffraction showesa B-type crystalline structure for all modified starches. With increasing reaction time, the relative crystallinity and endothermic enthalpy of the modified starches gradually decreases, whereas the melting peak temperatures and resistant starch contents increases. Slight differences are observed in thermal parameters, relative crystallinity, and branch chain length distribution between the modified native and acid-thinned starches. The digestibility of the modified starches is not affected by acid hydrolysis pretreatment, but is affected by the percentage of intermediate and long chains
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
the enzyme be a promising candidate for food industrial production of linear alpha-(1,4)-glucans and turanose as a next generation sweetener
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
food industry
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
industry
cyclodextrins are frequently utilized chemical substances in the food, pharmaceutical, cosmetics, and chemical industries. An enzymatic process for cyclodextrin production is developed by utilizing sucrose as raw material instead of corn starch. Cyclodextrin glucanotransferase from Paenibacillus macerans is applied to produce the cyclodextrins from linear alpha-(1,4)-glucans, which are obtained by Neisseria polysaccharea amylosucrase treatment on sucrose. The greatest cyclodextrin yield (21.1%, w/w) is achieved from a one-pot dual enzyme reaction at 40°C for 24 h. The maximum level of cyclodextrin production (15.1 mg/ml) is achieved with 0.5 M sucrose in a simultaneous mode of dual enzyme reaction, whereas the reaction with 0.1 M sucrose is the most efficient with regard to conversion yield. Dual enzyme synthesis of cyclodextrins is successfully carried out with no need of starch material. Efficient bioconversion process that does not require the high temperature necessary for starch liquefaction by thermostable alpha-amylase in conventional industrial processing
industry
the beta-carotene embedded amylose microparticles are prepared in one-step by utilizing the unique catalytic activity of amylosucrase from Deinococcus geothermalis, which synthesizes linear amylose chains using sucrose as the sole substrate. Synthesized amylose chains self-assembled with b-carotene to form well-defined spherical microparticles with an encapsulation yield of 65%. The synthetic method enables microparticle formation and beta-carotene encapsulation in one-step using amylosucrase and sucrose as the sole substrates, which indicates that the devised process may be cost-effective and suitable for industrial applications
industry
the enzyme has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization
industry
-
the beta-carotene embedded amylose microparticles are prepared in one-step by utilizing the unique catalytic activity of amylosucrase from Deinococcus geothermalis, which synthesizes linear amylose chains using sucrose as the sole substrate. Synthesized amylose chains self-assembled with b-carotene to form well-defined spherical microparticles with an encapsulation yield of 65%. The synthetic method enables microparticle formation and beta-carotene encapsulation in one-step using amylosucrase and sucrose as the sole substrates, which indicates that the devised process may be cost-effective and suitable for industrial applications
-
industry
-
the enzyme has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization
-
synthesis
-
mutant enzyme R226A, that is activated by the products it forms and yields twice as much insoluble glucan and lower quantities of by-products as the wild-type enzyme is a very promising enzyme for industrial synthesis of amylose-like polymers
synthesis
-
potentiality of amylosucrase in the design of amylodextrins with controlled morphology, structure, and physicochemical properties
synthesis
-
potential of amylosucrase in the design of original carbohydrate-based dendritic nanoparticles
synthesis
-
the enzyme can be used for synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule
synthesis
the enzyme can efficiently be used for synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule
synthesis
the enzyme might be useful for important tailoring reactions for the generation of bioactive compounds by glycosylation
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
D3A730
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
synthesis
immobilization enhances the efficiency of the glycosylation reaction and is therefore considered effective for industrial application in sustainable production of dihydroxybenzene glucosides
synthesis
isoquercitrin (quercetin-3-O-beta-D-glucopyranoside) has diverse biological functions, such as anti-oxidant and anticancer activity, but its use is limited by poor solubility and bioavailability. Enzymatically modified isoquercitrin (EMIQ) is a mixture of transglycosylated isoquercitrins that have better solubility and bioavailability than do quercetin and isoquercitrin. Amylosucrase (ASase), has transglycosylation activity to produce EMIQ. Both enzymes produce a variety of EMIQs including isoquercitrin, isoquercitrin-glucoside, isoquercitrin-diglucoside, and isoquercitrin-triglucoside. The enzyme has a higher bioconversion yield from isoquercitrin to EMIQ (97%). The yield of soquercitrin-triglucoside, which is the most bioavailable form is 46%. The enzyme can be used to synthesize EMIQ in a simple and specific process
synthesis
mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/Q437S/N439D/C445A only produces soluble oligosaccharides as no insoluble high molecular weight amylose is observed. The mutant enzyme is an attractive enzymatic tool that could offer interesting opportunities for the design of amylodextrins with controlled size
synthesis
the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate
synthesis
the beta-carotene embedded amylose microparticles are prepared in one-step by utilizing the unique catalytic activity of amylosucrase from Deinococcus geothermalis, which synthesizes linear amylose chains using sucrose as the sole substrate. Synthesized amylose chains self-assembled with b-carotene to form well-defined spherical microparticles with an encapsulation yield of 65%. The synthetic method enables microparticle formation and beta-carotene encapsulation in one-step using amylosucrase and sucrose as the sole substrates, which indicates that the devised process may be cost-effective and suitable for industrial applications
synthesis
transglycosylation reactions with amylosucrase from Deinococcus geothermalis constitute an efficient and economical method to produce alpha-glucosyl flavonoids
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate
-
synthesis
-
the enzyme might be useful for important tailoring reactions for the generation of bioactive compounds by glycosylation
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
the beta-carotene embedded amylose microparticles are prepared in one-step by utilizing the unique catalytic activity of amylosucrase from Deinococcus geothermalis, which synthesizes linear amylose chains using sucrose as the sole substrate. Synthesized amylose chains self-assembled with b-carotene to form well-defined spherical microparticles with an encapsulation yield of 65%. The synthetic method enables microparticle formation and beta-carotene encapsulation in one-step using amylosucrase and sucrose as the sole substrates, which indicates that the devised process may be cost-effective and suitable for industrial applications
-
synthesis
-
isoquercitrin (quercetin-3-O-beta-D-glucopyranoside) has diverse biological functions, such as anti-oxidant and anticancer activity, but its use is limited by poor solubility and bioavailability. Enzymatically modified isoquercitrin (EMIQ) is a mixture of transglycosylated isoquercitrins that have better solubility and bioavailability than do quercetin and isoquercitrin. Amylosucrase (ASase), has transglycosylation activity to produce EMIQ. Both enzymes produce a variety of EMIQs including isoquercitrin, isoquercitrin-glucoside, isoquercitrin-diglucoside, and isoquercitrin-triglucoside. The enzyme has a higher bioconversion yield from isoquercitrin to EMIQ (97%). The yield of soquercitrin-triglucoside, which is the most bioavailable form is 46%. The enzyme can be used to synthesize EMIQ in a simple and specific process
-
synthesis
-
transglycosylation reactions with amylosucrase from Deinococcus geothermalis constitute an efficient and economical method to produce alpha-glucosyl flavonoids
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
immobilization enhances the efficiency of the glycosylation reaction and is therefore considered effective for industrial application in sustainable production of dihydroxybenzene glucosides
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
synthesis
-
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences
-
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Enzymatic synthesis and reactions of a sucrose isomer alpha-D-galactopyranosyl-beta-D-fructofuranoside
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1957
Neisseria perflava
brenda
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Neisseria perflava amylosucrase: characterization of its product polysaccharide and a study of its inhibition by sucrose derivatives
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Neisseria perflava
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Okada, G.; Hehre, E.J.
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1973
Neisseria perflava
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Studies on a recombinant amylosucrase
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Neisseria polysaccharea
-
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De Montalk, G.P.; Remaud-Simeon, M.; Willemot, R.M.; Planchot, V.; Monsan, P.
Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme
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Characterization of the activator effect of glycogen on amylosucrase from Neisseria polysaccharea
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2000
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Skov, L.K.; Mirza, O.; Henriksen, A.; de Montalk, G.P.; Remaud-Simeon, M.; Sarcabal, P.; Willemot, R.M.; Monsan, P.; Gajhede, M.
Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family
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2001
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Skov, L.K.; Mirza, O.; Sprogoe, D.; Dar, I.; Remaud-Simeon, M.; Albenne, C.; Monsan, P.; Gajhede, M.
Oligosaccharide and Sucrose Complexes of Amylosucrase
J. Biol. Chem.
277
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2002
Neisseria polysaccharea (Q9ZEU2)
brenda
Mirza, O.; Skov, L.K.; Remaud-Simeon, M.; Potocki de Montalk, G.; Albenne, C.; Monsan, P.; Gajhede, M.
Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose
Biochemistry
40
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2001
Neisseria polysaccharea
brenda
Albenne, C.; Potocki De Montalk, G.; Monsan, P.; Skov, L.; Mirza, O.; Gajhede, M.; Remaud-Simeon, M.
Site-directed mutagenesis of key amino acids in the active site of amylosucrase from Neisseria polysaccharea
Biologia (Bratisl. )
57
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2002
Neisseria polysaccharea
-
brenda
Jensen, M.H.; Mirza, O.; Albenne, C.; Remaud-Simeon, M.; Monsan, P.; Gajhede, M.; Skov, L.K.
Crystal structure of the covalent intermediate of amylosucrase from Neisseria polysaccharea
Biochemistry
43
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2004
Neisseria polysaccharea
brenda
Potocki-Veronese, G.; Putaux, J.L.; Dupeyre, D.; Albenne, C.; Remaud-Simeon, M.; Monsan, P.; Buleon, A.
Amylose synthesized in vitro by amylosucrase: morphology, structure, and properties
Biomacromolecules
6
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2005
Neisseria polysaccharea
brenda
van der Veen, B.A.; Potocki-Veronese, G.; Albenne, C.; Joucla, G.; Monsan, P.; Remaud-Simeon, M.
Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity
FEBS Lett.
560
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2004
Neisseria polysaccharea
brenda
Pizzut-Serin, S.; Potocki-Veronese, G.; van der Veen, B.A.; Albenne, C.; Monsan, P.; Remaud-Simeon, M.
Characterisation of a novel amylosucrase from Deinococcus radiodurans
FEBS Lett.
579
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2005
Deinococcus radiodurans
brenda
Albenne, C.; Skov, L.K.; Mirza, O.; Gajhede, M.; Feller, G.; D'Amico, S.; Andre, G.; Potocki-Veronese, G.; van der Veen, B.A.; Monsan, P.; Remaud-Simeon, M.
Molecular basis of the amylose-like polymer formation catalyzed by Neisseria polysaccharea amylosucrase
J. Biol. Chem.
279
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2004
Neisseria polysaccharea
brenda
Rolland-Sabate, A.; Colonna, P.; Potocki-Veronese, G.; Monsan, P.; Planchot, V.
Elongation and insolubilisation of ?-glucans by the action of Neisseria polysaccharea amylosucrase.
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40
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2004
Neisseria polysaccharea
brenda
Putaux, J.L.; Potocki-Veronese, G.; Remaud-Simeon, M.; Buleon, A.
alpha-D-Glucan-based dendritic nanoparticles prepared by in vitro enzymatic chain extension of glycogen
Biomacromolecules
7
1720-1728
2006
Neisseria polysaccharea
brenda
van der Veen, B.A.; Skov, L.K.; Potocki-Veronese, G.; Gajhede, M.; Monsan, P.; Remaud-Simeon, M.
Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution
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273
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2006
Neisseria polysaccharea (Q9ZEU2)
brenda
Albenne, C.; Skov, L.K.; Tran, V.; Gajhede, M.; Monsan, P.; Remaud-Simeon, M.; Andre-Leroux, G.
Towards the molecular understanding of glycogen elongation by amylosucrase
Proteins
66
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2007
Neisseria polysaccharea
brenda
Emond, S.; Mondeil, S.; Jaziri, K.; Andre, I.; Monsan, P.; Remaud-Simeon, M.; Potocki-Veronese, G.
Cloning, purification and characterization of a thermostable amylosucrase from Deinococcus geothermalis
FEMS Microbiol. Lett.
285
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2008
Deinococcus geothermalis
brenda
Emond, S.; Potocki-Veronese, G.; Mondon, P.; Bouayadi, K.; Kharrat, H.; Monsan, P.; Remaud-Simeon, M.
Optimized and automated protocols for high-throughput screening of amylosucrase libraries
J. Biomol. Screen.
12
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2007
Neisseria polysaccharea (Q9ZEU2)
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Emond, S.; Andre, I.; Jaziri, K.; Potocki-Veronese, G.; Mondon, P.; Bouayadi, K.; Kharrat, H.; Monsan, P.; Remaud-Simeon, M.
Combinatorial engineering to enhance thermostability of amylosucrase
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17
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2008
Deinococcus radiodurans
brenda
Schneider, J.; Fricke, C.; Overwin, H.; Hofmann, B.; Hofer, B.
Generation of amylosucrase variants that terminate catalysis of acceptor elongation at the di- or trisaccharide stage
Appl. Environ. Microbiol.
75
7453-7460
2009
Neisseria polysaccharea (Q9ZEU2)
brenda
Jung, J.H.; Seo, D.H.; Ha, S.J.; Song, M.C.; Cha, J.; Yoo, S.H.; Kim, T.J.; Baek, N.I.; Baik, M.Y.; Park, C.S.
Enzymatic synthesis of salicin glycosides through transglycosylation catalyzed by amylosucrases from Deinococcus geothermalis and Neisseria polysaccharea
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344
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2009
Deinococcus geothermalis, Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Seo, D.; Jung, J.; Ha, S.; Song, M.; Cha, J.; Yoo, S.; Kim, T.; Baek, N.; Park, C.
Highly selective biotransformation of arbutin to arbutin-alpha-glucoside using amylosucrase from Deinococcus geothermalis DSM 11300
J. Mol. Catal. B
60
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2009
Deinococcus geothermalis
-
brenda
Seo, D.H.; Jung, J.H.; Ha, S.J.; Cho, H.K.; Jung, D.H.; Kim, T.J.; Baek, N.I.; Yoo, S.H.; Park, C.S.
High-yield enzymatic bioconversion of hydroquinone to alpha-arbutin, a powerful skin lightening agent, by amylosucrase
Appl. Microbiol. Biotechnol.
94
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2012
Deinococcus geothermalis
brenda
Wang, R.; Kim, J.H.; Kim, B.S.; Park, C.S.; Yoo, S.H.
Preparation and characterization of non-covalently immobilized amylosucrase using a pH-dependent autoprecipitating carrier
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102
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2011
Neisseria polysaccharea, Neisseria polysaccharea ATCC 43768
brenda
Cho, H.K.; Kim, H.H.; Seo, D.H.; Jung, J.H.; Park, J.H.; Baek, N.I.; Kim, M.J.; Yoo, S.H.; Cha, J.; Kim, Y.R.; Park, C.S.
Biosynthesis of (+)-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300
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49
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2011
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Deinococcus geothermalis DSM 11300 (Q1J0W0), Deinococcus geothermalis DSM 11300
brenda
Kim, J.H.; Wang, R.; Lee, W.H.; Park, C.S.; Lee, S.; Yoo, S.H.
One-pot synthesis of cycloamyloses from sucrose by dual enzyme treatment: combined reaction of amylosucrase and 4-alpha-glucanotransferase
J. Agric. Food Chem.
59
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2011
Neisseria polysaccharea
brenda
Guerin, F.; Barbe, S.; Pizzut-Serin, S.; Potocki-Veronese, G.; Guieysse, D.; Guillet, V.; Monsan, P.; Mourey, L.; Remaud-Simeon, M.; Andre, I.; Tranier, S.
Structural investigation of the thermostability and product specificity of amylosucrase from the bacterium Deinococcus geothermalis
J. Biol. Chem.
287
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2012
Deinococcus geothermalis, Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Seo, D.H.; Jung, J.H.; Choi, H.C.; Cho, H.K.; Kim, H.H.; Ha, S.J.; Yoo, S.H.; Cha, J.; Park, C.S.
Functional expression of amylosucrase, a glucan-synthesizing enzyme, from Arthrobacter chlorophenolicus A6
J. Microbiol. Biotechnol.
22
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2012
Pseudarthrobacter chlorophenolicus, Pseudarthrobacter chlorophenolicus A6 / DSM 12829
brenda
Liu, M.; Wang, S.; Sun, T.; Su, J.; Zhang, Y.; Yue, J.; Sun, Z.
Insight into the structure, dynamics and the unfolding property of amylosucrases: implications of rational engineering on thermostability
PLoS ONE
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e40441
2012
Deinococcus geothermalis
brenda
Kim, H.; Jung, J.; Seo, D.; Ha, S.; Yoo, S.; Kim, C.; Park, C.
Novel enzymatic production of trehalose from sucrose using amylosucrase and maltooligosyltrehalose synthase-trehalohydrolase
World J. Microbiol. Biotechnol.
27
2851-2856
2011
Deinococcus geothermalis
brenda
Skov, L.K.; Pizzut-Serin, S.; Remaud-Simeon, M.; Ernst, H.A.; Gajhede, M.; Mirza, O.
The structure of amylosucrase from Deinococcus radiodurans has an unusual open active-site topology
Acta Crystallogr. Sect. F
69
973-978
2013
Deinococcus radiodurans
brenda
Jeong, J.W.; Seo, D.H.; Jung, J.H.; Park, J.H.; Baek, N.I.; Kim, M.J.; Park, C.S.
Biosynthesis of glucosyl glycerol, a compatible solute, using intermolecular transglycosylation activity of amylosucrase from Methylobacillus flagellatus KT
Appl. Biochem. Biotechnol.
173
904-917
2014
Methylobacillus flagellatus, Methylobacillus flagellatus ATCC 51484
brenda
But, S.Y.; Khmelenina, V.N.; Reshetnikov, A.S.; Mustakhimov, I.I.; Kalyuzhnaya, M.G.; Trotsenko, Y.A.
Sucrose metabolism in halotolerant methanotroph Methylomicrobium alcaliphilum 20Z
Arch. Microbiol.
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2015
Methylotuvimicrobium alcaliphilum (G4T024), Methylotuvimicrobium alcaliphilum 20Z (G4T024), Methylotuvimicrobium alcaliphilum 20Z
brenda
Cambon, E.; Barbe, S.; Pizzut-Serin, S.; Remaud-Simeon, M.; Andre, I.
Essential role of amino acid position 226 in oligosaccharide elongation by amylosucrase from Neisseria polysaccharea
Biotechnol. Bioeng.
111
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2014
Neisseria polysaccharea
brenda
Kim, B.S.; Kim, H.S.; Yoo, S.H.
Characterization of enzymatically modified rice and barley starches with amylosucrase at scale-up production
Carbohydr. Polym.
125
61-68
2015
Neisseria polysaccharea
brenda
Daude, D.; Champion, E.; Morel, S.; Guieysse, D.; Remaud-Simeon, M.; Andre, I.
Probing substrate promiscuity of amylosucrase from Neisseria polysaccharea
ChemCatChem
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2288-2295
2013
Neisseria polysaccharea (Q9ZEU2)
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brenda
Seo, D.H.; Jung, J.H.; Jung, D.H.; Park, S.; Yoo, S.H.; Kim, Y.R.; Park, C.S.
An unusual chimeric amylosucrase generated by domain-swapping mutagenesis
Enzyme Microb. Technol.
86
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2016
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Kim, B.K.; Kim, H.I.; Moon, T.W.; Choi, S.J.
Branch chain elongation by amylosucrase: production of waxy corn starch with a slow digestion property
Food Chem.
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2014
Neisseria polysaccharea (Q9ZEU2)
brenda
Kim, M.; Seo, D.; Jung, J.; Jung, D.; Joe, M.; Lim, S.; Lee, J.; Park, C.
Molecular cloning and expression of amylosucrase from highly radiation-resistant Deinococcus radiopugnans
Food Sci. Biotechnol.
23
2007-2012
2014
Deinococcus radiopugnans, Deinococcus radiopugnans ATCC 19172
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brenda
Overwin, H.; Wray, V.; Hofer, B.
Biotransformation of phloretin by amylosucrase yields three novel dihydrochalcone glucosides
J. Biotechnol.
211
103-106
2015
Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea, Neisseria polysaccharea ATCC 43768 (Q9ZEU2)
brenda
Perez-Cenci, M.; Salerno, G.L.
Functional characterization of Synechococcus amylosucrase and fructokinase encoding genes discovers two novel actors on the stage of cyanobacterial sucrose metabolism
Plant Sci.
224
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2014
Synechococcus sp. (B1XIU7), Synechococcus sp.
brenda
Huang, X.F.; Nazarian-Firouzabadi, F.; Vincken, J.P.; Ji, Q.; Visser, R.G.; Trindade, L.M.
Expression of an amylosucrase gene in potato results in larger starch granules with novel properties
Planta
240
409-421
2014
Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Liu, M.; Li, P.; Liu, B.; Su, J.; Wang, C.
Insights into the working mechanism and unfolding property of Arthrobacter chlorophenolicus amylosucrase
Prog. Biochem. Biophys.
40
565-577
2013
Pseudarthrobacter chlorophenolicus
-
brenda
Daude, D.; Topham, C.M.; Remaud-Simeon, M.; Andre, I.
Probing impact of active site residue mutations on stability and activity of Neisseria polysaccharea amylosucrase
Protein Sci.
22
1754-1765
2013
Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Tian, Y.; Xu, W.; Zhang, W.; Zhang, T.; Guang, C.; Mu, W.
Amylosucrase as a transglucosylation tool From molecular features to bioengineering applications
Biotechnol. Adv.
36
1540-1552
2018
Deinococcus radiopugnans, Cellulomonas carbonis (A0A0A0BUC7), Alteromonas macleodii (B6F2H1), Pseudarthrobacter chlorophenolicus (B8H6N5), Neisseria subflava (D3A730), Methylotuvimicrobium alcaliphilum (G4T024), Methylobacillus flagellatus (Q1GY12), Deinococcus geothermalis (Q1J0W0), Deinococcus radiodurans (Q9RVT9), Neisseria polysaccharea (Q9ZEU2), Synechococcus sp. (SMQ77851), Neisseria subflava NJ9703 (D3A730), Deinococcus geothermalis DSM 11300 (Q1J0W0), Cellulomonas carbonis T26 (A0A0A0BUC7), Methylotuvimicrobium alcaliphilum DSM 19304 (G4T024), Deinococcus radiodurans ATCC 13939 (Q9RVT9), Pseudarthrobacter chlorophenolicus DSM 12829 (B8H6N5)
brenda
Letona, C.A.M.; Park, C.S.; Kim, Y.R.
Amylosucrase-mediated ?-carotene encapsulation in amylose microparticles
Biotechnol. Prog.
33
1640-1646
2017
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Deinococcus geothermalis DSM 11300 (Q1J0W0)
brenda
Verges, A.; Barbe, S.; Cambon, E.; Moulis, C.; Tranier, S.; Remaud-Simeon, M.; Andre, I.
Engineering of anp efficient mutant of Neisseria polysaccharea amylosucrase for the synthesis of controlled size maltooligosaccharides
Carbohydr. Polym.
173
403-411
2017
Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Rha, C.S.; Choi, J.M.; Jung, Y.S.; Kim, E.R.; Ko, M.J.; Seo, D.H.; Kim, D.O.; Park, C.S.
High-efficiency enzymatic production of alpha-isoquercitrin glucosides by amylosucrase from Deinococcus geothermalis
Enzyme Microb. Technol.
120
84-90
2019
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Deinococcus geothermalis DSM 11300 (Q1J0W0)
brenda
Rha, C.S.; Jung, Y.S.; Seo, D.H.; Kim, D.O.; Park, C.S.
Site-specific alpha-glycosylation of hydroxyflavones and hydroxyflavanones by amylosucrase from Deinococcus geothermalis
Enzyme Microb. Technol.
129
109361
2019
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Deinococcus geothermalis DSM 11300 (Q1J0W0)
brenda
Chin, Y.; Jang, S.; Shin, H.; Kim, T.; Kim, S.; Park, C.; Seo, D.
Heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum for luteolin glucoside production
Enzyme Microb. Technol.
135
109505
2020
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Deinococcus geothermalis DSM 11300 (Q1J0W0)
brenda
Zhang, H.; Zhou, X.; He, J.; Wang, T.; Luo, X.; Wang, L.; Wang, R.; Chen, Z.
Impact of amylosucrase modification on the structural and physicochemical properties of native and acid-thinned waxy corn starch
Food Chem.
220
413-419
2017
Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Seo, D.H.; Yoo, S.H.; Choi, S.J.; Kim, Y.R.; Park, C.S.
Versatile biotechnological applications of amylosucrase, a novel glucosyltransferase
Food Sci. Biotechnol.
29
1-16
2020
Synechococcus sp. PCC 7002, Bifidobacterium thermophilum, Cellulomonas carbonis (A0A0A0BUC7), Deinococcus radiopugnans (A0A4P8XUU6), Alteromonas stellipolaris (B6F2G7), Alteromonas macleodii (B6F2H1), Pseudarthrobacter chlorophenolicus (B8H6N5), Neisseria subflava (D3A730), Methylotuvimicrobium alcaliphilum (G4T024), Methylobacillus flagellatus (Q1GY12), Deinococcus geothermalis (Q1J0W0), Deinococcus radiodurans (Q9RVT9), Neisseria polysaccharea (Q9ZEU2), Pseudarthrobacter chlorophenolicus ATCC 700700 (B8H6N5), Alteromonas stellipolaris KCTC 12195 (B6F2G7), Neisseria polysaccharea ATCC 43768 (Q9ZEU2), Deinococcus geothermalis DSM 11300 (Q1J0W0), Cellulomonas carbonis T26 (A0A0A0BUC7), Methylotuvimicrobium alcaliphilum 20Z (G4T024), Deinococcus radiodurans ATCC 13939 (Q9RVT9), Neisseria subflava ATCC 49275 (D3A730), Alteromonas macleodii KCTC 2957 (B6F2H1), Deinococcus radiopugnans ATCC 19172 (A0A4P8XUU6), Bifidobacterium thermophilum ATCC 25525
brenda
Park, M.O.; Chandrasekaran, M.; Yoo, S.H.
Expression, purification, and characterization of a novel amylosucrase from Neisseria subflava
Int. J. Biol. Macromol.
109
160-166
2018
Neisseria subflava (D3A730), Neisseria subflava, Neisseria subflava ATCC 49275 (D3A730)
brenda
Zhu, X.; Tian, Y.; Xu, W.; Bai, Y.; Zhang, T.; Mu, W.
Biochemical characterization of a highly thermostable amylosucrase from Truepera radiovictrix DSM 17093
Int. J. Biol. Macromol.
116
744-752
2018
Truepera radiovictrix (D7CVD0), Truepera radiovictrix DSM 17093 (D7CVD0), Truepera radiovictrix DSM 17093
brenda
Zhang, H.; Zhou, X.; Wang, T.; Luo, X.; Wang, L.; Li, Y.; Wang, R.; Chen, Z.
New insights into the action mode of amylosucrase on amylopectin
Int. J. Biol. Macromol.
88
380-384
2016
Neisseria polysaccharea (Q9ZEU2)
brenda
Koh, D.W.; Park, M.O.; Choi, S.W.; Lee, B.H.; Yoo, S.H.
Efficient biocatalytic production of cyclodextrins by combined action of amylosucrase and cyclodextrin glucanotransferase
J. Agric. Food Chem.
64
4371-4375
2016
Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda
Wang, Y.; Xu, W.; Bai, Y.; Zhang, T.; Jiang, B.; Mu, W.
Identification of an alpha-(1,4)-glucan-synthesizing amylosucrase from Cellulomonas carboniz T26
J. Agric. Food Chem.
65
2110-2119
2017
Cellulomonas carbonis (A0A0A0BUC7), Cellulomonas carbonis T26 (A0A0A0BUC7)
brenda
Tian, Y.; Xu, W.; Guang, C.; Zhang, W.; Mu, W.
Thermostable amylosucrase from Calidithermus timidus DSM 17022 insight into its characteristics and tetrameric conformation
J. Agric. Food Chem.
67
9868-9876
2019
Calidithermus timidus (WP_018466847), Calidithermus timidus DSM 17022 (WP_018466847), Calidithermus timidus DSM 17022
brenda
Zhu, L.; Jiang, D.; Zhou, Y.; Lu, Y.; Fan, Y.; Chen, X.
Batch-feeding whole-cell catalytic synthesis of alpha-arbutin by amylosucrase from Xanthomonas campestris
J. Ind. Microbiol. Biotechnol.
46
759-767
2019
Xanthomonas campestris pv. campestris (A0A0H2X5U3), Xanthomonas campestris pv. campestris 8004 (A0A0H2X5U3)
brenda
Lee, H.S.; Kim, T.S.; Parajuli, P.; Pandey, R.P.; Sohng, J.K.
Sustainable production of dihydroxybenzene glucosides using immobilized amylosucrase from Deinococcus geothermalis
J. Microbiol. Biotechnol.
28
1447-1456
2018
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Deinococcus geothermalis DSM 11300 (Q1J0W0), Deinococcus geothermalis DSM 11300
brenda
Jang, S.W.; Cho, C.H.; Jung, Y.S.; Rha, C.; Nam, T.G.; Kim, D.O.; Lee, Y.G.; Baek, N.I.; Park, C.S.; Lee, B.H.; Lee, S.Y.; Shin, H.S.; Seo, D.H.
Enzymatic synthesis of ?-flavone glucoside via regioselective transglucosylation by amylosucrase from Deinococcus geothermalis
PLoS ONE
13
e0207466
2018
Deinococcus geothermalis (Q1J0W0), Deinococcus geothermalis, Deinococcus geothermalis DSM 11300 (Q1J0W0)
brenda
Verges, A.; Cambon, E.; Barbe, S.; Moulis, C.; Remaud-Simeon, M.; Andre, I.
Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucrase
Protein Sci.
26
566-577
2017
Neisseria polysaccharea (Q9ZEU2), Neisseria polysaccharea
brenda