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Literature summary for 2.4.1.4 extracted from

  • Okada, G.; Hehre, E.J.
    New studies on amylosucrase, a bacterial alpha-D-glucosylase that directly converts sucrose to a glycogen-like alpha-glucan (1974), J. Biol. Chem., 249, 126-135.
    View publication on PubMed
Search for more literature for 2.4.1.4

Activating Compound

Activating Compound Comment Organism Structure
additional information no ADP, UDP, ADPglucose or UDPglucose involved Neisseria perflava

General Stability

General Stability Organism
rapid freezing or deep freezing inactivates Neisseria perflava

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
 sucrose
-
 Neisseria perflava

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Neisseria perflava
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (1,4-alpha-D-glucosyl)n Neisseria perflava involved in biosynthesis of amylopectin-glycogen type polysaccharide D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Neisseria perflava
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neisseria perflava

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.71
-
-
 Neisseria perflava

Storage Stability

Storage Stability Organism
2-4°C, t1/2: 15-20 h Neisseria perflava

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (1,4-alpha-D-glucosyl)n needs mussel or sweet corn glycogen, or corn amylopectin as primer molecule, sucrose alone is no substrate, beta-D-galactosylsucrose can replace sucrose Neisseria perflava D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?
sucrose + (1,4-alpha-D-glucosyl)n needs glucan from Neisseria sp. as primer molecule Neisseria perflava D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?
sucrose + (1,4-alpha-D-glucosyl)n no activity with melezitose Neisseria perflava D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?
sucrose + (1,4-alpha-D-glucosyl)n involved in biosynthesis of amylopectin-glycogen type polysaccharide Neisseria perflava D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Neisseria perflava

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 inactivation within 10 min Neisseria perflava