Literature summary for 2.4.1.4 extracted from

Potocki de Montalk, G.; Remaud-Simeon, M.; Willemot, R.M.; Sarcabal, P.; Planchot, V.; Monsan, P.
Amylosucrase from Neisseria polysaccharea: novel catalytic properties (2000), FEBS Lett., 471, 219-223.

Search for more literature for 2.4.1.4

Inhibitors

Inhibitors	Comment	Organism	Structure
D-fructose	competitive inhibition	Neisseria polysaccharea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.9	-	sucrose	initial sucrose concentration lower than 20 mM	Neisseria polysaccharea
38.7	-	sucrose	initial sucrose concentration higehr than 20 mM, sucrose hydrolysis	Neisseria polysaccharea
50.2	-	sucrose	initial sucrose concentration higher than 20 mM, sucrose consumption	Neisseria polysaccharea
387	-	sucrose	initial sucrose concentration higher than 20 mM, polymerization	Neisseria polysaccharea

Organism

Organism	UniProt	Comment	Textmining
Neisseria polysaccharea	-	-	-

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	native enzyme, half-life: 15-20 h	Neisseria polysaccharea
30	-	recombinant enzyme, half-life: 21 h	Neisseria polysaccharea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.167	-	sucrose	initial sucrose concentration lower than 20 mM, polymerization	Neisseria polysaccharea
0.333	-	sucrose	initial sucrose concentration lower than 20 mM, sucrose hydrolysis	Neisseria polysaccharea
0.55	-	sucrose	initial sucrose concentration lower than 20 mM, sucrose consumption	Neisseria polysaccharea
0.55	-	sucrose	initial sucrose concentration higher than 20 mM, sucrose hydrolysis	Neisseria polysaccharea
1.28	-	sucrose	initial sucrose concentration higher than 20 mM, sucrose consumption	Neisseria polysaccharea
1.88	-	sucrose	initial sucrose concentration higher than 20 mM, polymerization	Neisseria polysaccharea

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
14	-	D-fructose	-	Neisseria polysaccharea

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Release 2023.1 (January 2023)