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Literature summary for 2.4.1.4 extracted from

  • Cho, H.K.; Kim, H.H.; Seo, D.H.; Jung, J.H.; Park, J.H.; Baek, N.I.; Kim, M.J.; Yoo, S.H.; Cha, J.; Kim, Y.R.; Park, C.S.
    Biosynthesis of (+)-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300 (2011), Enzyme Microb. Technol., 49, 246-253.
    View publication on PubMed
Search for more literature for 2.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene dgas, expression of GST-tagged DGAS in Escherichia coli Deinococcus geothermalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->4)-alpha-D-glucosyl]n Deinococcus geothermalis
-
 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->4)-alpha-D-glucosyl]n Deinococcus geothermalis DSM 11300
-
 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Deinococcus geothermalis Q1J0W0 gene dgas
-
Deinococcus geothermalis DSM 11300 Q1J0W0 gene dgas
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography Deinococcus geothermalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NMR product analysis, overview Deinococcus geothermalis ?
-
 ?
additional information NMR product analysis, overview Deinococcus geothermalis DSM 11300 ?
-
 ?
sucrose + (+)-catechin
-
 Deinococcus geothermalis D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
 ?
sucrose + (+)-catechin
-
 Deinococcus geothermalis DSM 11300 D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
 ?
sucrose + maltose the enzyme also produces (+)-catechin maltooligosaccharides Deinococcus geothermalis D-fructose + maltotriose
-
 ?
sucrose + maltose the enzyme also produces (+)-catechin maltooligosaccharides Deinococcus geothermalis DSM 11300 D-fructose + maltotriose
-
 ?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
 Deinococcus geothermalis D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->4)-alpha-D-glucosyl]n a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity Deinococcus geothermalis D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->4)-alpha-D-glucosyl]n
-
 Deinococcus geothermalis DSM 11300 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->4)-alpha-D-glucosyl]n a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity Deinococcus geothermalis DSM 11300 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Synonyms

Synonyms Comment Organism
ASASE
-
 Deinococcus geothermalis
DGAS
-
 Deinococcus geothermalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Deinococcus geothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Deinococcus geothermalis