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Literature summary for 2.4.1.4 extracted from
- Biosynthesis of glucosyl glycerol, a compatible solute, using intermolecular transglycosylation activity of amylosucrase from Methylobacillus flagellatus KT (2014), Appl. Biochem. Biotechnol., 173, 904-917.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mfas, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Methylobacillus flagellatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74560 | - |
sequence calculation | Methylobacillus flagellatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylobacillus flagellatus | - |
gene mfas | - |
| Methylobacillus flagellatus ATCC 51484 | - |
gene mfas | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Methylobacillus flagellatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the purified recombinant enzyme displays a typical amylosucrase activity by the demonstration of multiple activities of hydrolysis, isomerization, and polymerization. The enzyme also shows sucrose hydrolysis activity | Methylobacillus flagellatus | ? | - |
? |  |
| additional information | the purified recombinant enzyme displays a typical amylosucrase activity by the demonstration of multiple activities of hydrolysis, isomerization, and polymerization. The enzyme also shows sucrose hydrolysis activity | Methylobacillus flagellatus ATCC 51484 | ? | - |
? | |
| sucrose + glycerol | glycerol is transglycosylated by the intermolecular transglycosylation activity of MFAS. The two major products are determined to be (2S)-1-O-alpha-D-glucosyl-glycerol or (2R)-1-O-alpha-D-glucosyl-glycerol, and 2-O-alpha-D-glucosyl-glycerol, in which a glucose molecule is linked to glycerol via an alpha-glycosidic linkage, NMR identification | Methylobacillus flagellatus | (2S)-1-O-alpha-D-glucosyl-glycerol + (2R)-1-O-alpha-D-glucosyl-glycerol + 2-O-alpha-D-glucosyl-glycerol | - |
? | |
| sucrose + glycerol | glycerol is transglycosylated by the intermolecular transglycosylation activity of MFAS. The two major products are determined to be (2S)-1-O-alpha-D-glucosyl-glycerol or (2R)-1-O-alpha-D-glucosyl-glycerol, and 2-O-alpha-D-glucosyl-glycerol, in which a glucose molecule is linked to glycerol via an alpha-glycosidic linkage, NMR identification | Methylobacillus flagellatus ATCC 51484 | (2S)-1-O-alpha-D-glucosyl-glycerol + (2R)-1-O-alpha-D-glucosyl-glycerol + 2-O-alpha-D-glucosyl-glycerol | - |
? | |
| sucrose + [(1->4)-alpha-D-glucosyl]n | the enzyme shows polymerization activity using sucrose as a sole substrate | Methylobacillus flagellatus | D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
| sucrose + [(1->4)-alpha-D-glucosyl]n | the enzyme shows polymerization activity using sucrose as a sole substrate | Methylobacillus flagellatus ATCC 51484 | D-fructose + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASASE | - |
Methylobacillus flagellatus |
| MFAS | - |
Methylobacillus flagellatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
assay at | Methylobacillus flagellatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | activity range, profile overview | Methylobacillus flagellatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life of 19.3 min | Methylobacillus flagellatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Methylobacillus flagellatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | activity range, profile overview | Methylobacillus flagellatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the putative MFAS protein shares not only catalytic residues, but also five consensus regions (CR I-V), with other ASases, although the overall protein shows a low level of amino acid sequence homology when compared with known ASases (ACAS, AMAS, DGAS, DRAS, and NPAS), overview | Methylobacillus flagellatus |
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