Information on EC 3.5.4.4 - adenosine deaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
3.5.4.4
-
RECOMMENDED NAME
GeneOntology No.
adenosine deaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
adenosine + H2O = inosine + NH3
show the reaction diagram
-
-
-
-
adenosine + H2O = inosine + NH3
show the reaction diagram
reaction mechanism, structure-function relationship of ADA and dipeptidyl peptidase IV
-
adenosine + H2O = inosine + NH3
show the reaction diagram
structure-activity relationships
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
amidine hydrolysis
-
-
Deamination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenine and adenosine salvage III
-
-
adenine and adenosine salvage V
-
-
adenosine nucleotides degradation II
-
-
Metabolic pathways
-
-
purine deoxyribonucleosides degradation
-
-
purine deoxyribonucleosides degradation I
-
-
Purine metabolism
-
-
purine metabolism
-
-
purine ribonucleosides degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
adenosine aminohydrolase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ADA
Q5ZKP6
-
ADA
P00813
-
ADA
P03958
-
ADA
Mus musculus C57BL/6
-
-
-
ADA
-
-
ADA
A5KE01
-
ADA
-
-
ADA1
-
ADA2 isoenzyme in cattle is absent
ADA1
P00813
isozyme
ADA2
-
-
ADA2
Q9NZK5
isozyme
Adenosine aminohydrolase
-
-
-
-
Adenosine aminohydrolase
-
-
adenosine deaminase
Q8LPL7
-
adenosine deaminase
-
-
adenosine deaminase
-
-
adenosine deaminase
P56658
-
adenosine deaminase
-
-
adenosine deaminase
Q5ZKP6
-
adenosine deaminase
P00813
-
adenosine deaminase
-
-
adenosine deaminase
P03958
-
adenosine deaminase
-
-
adenosine deaminase
A5KE01
-
adenosine deaminase
-
-
adenosine deaminase
-
-
adenosine deaminase
-
-
adenosine deaminase
-
-
adenosine deaminase 1
-
-
adenosine deaminase 1
P00813
isozyme
adenosine deaminase 2
-
-
adenosine deaminase 2
-
-
adenosine deaminase 2
Q9NZK5
isozyme
ciADA
-
-
LADA
-
-
MadA
-
-
MJ1541
Q58936
locus name
PvADA
A5KE01
-
SADA
-
-
TadA
-
-
CAS REGISTRY NUMBER
COMMENTARY
9026-93-1
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Azotobacter vinelandii 0
strain 0
-
-
Manually annotated by BRENDA team
NCIB 8122
-
-
Manually annotated by BRENDA team
cattle naturally infected with Theileria annulata
-
-
Manually annotated by BRENDA team
mucosa, commercial preparation
-
-
Manually annotated by BRENDA team
small and large isozymes SADA and LADA
-
-
Manually annotated by BRENDA team
type small and large
-
-
Manually annotated by BRENDA team
eu- and hyperthyroid guinea pigs
-
-
Manually annotated by BRENDA team
Babcock Venkateswara 380, BV 380 breed
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
CCRF-CEM; CCRF-HSB-2; normal B-cells and T-cell lines MOLT-4; RPMI-8402
-
-
Manually annotated by BRENDA team
CCRF-CEM; expression in Escherichia coli; normal B-cells and T-cell lines MOLT-4; RPMI-8402
-
-
Manually annotated by BRENDA team
CCRF-CEM; larger enzyme: El, smaller enzyme: Es; normal B-cells and T-cell lines MOLT-4; RPMI-8402
-
-
Manually annotated by BRENDA team
CCRF-CEM; normal B-cells and T-cell lines MOLT-4; RPMI-8402
-
-
Manually annotated by BRENDA team
CCRF-CEM; normal B-cells and T-cell lines MOLT-4; RPMI-8402; type 1, 2 and 2-1
-
-
Manually annotated by BRENDA team
CCRF-CEM; normal B-cells and T-cell lines MOLT-4; RPMI-8402; type a and b
-
-
Manually annotated by BRENDA team
CCRF-CEM; normal B-cells and T-cell lines MOLT-4; RPMI-8402; type small, intermediate and large
-
-
Manually annotated by BRENDA team
female, isozymes ADA1 and ADA2
-
-
Manually annotated by BRENDA team
healthy and multiple sclerosis patients
-
-
Manually annotated by BRENDA team
healthy persons and tuberculous or non-infectious meningitis patients
-
-
Manually annotated by BRENDA team
isoenzymes ADA1 and ADA2
-
-
Manually annotated by BRENDA team
isozyme ADA2
SwissProt
Manually annotated by BRENDA team
isozymes ADA1 and ADA2
-
-
Manually annotated by BRENDA team
patients with familial mediterranean fever
-
-
Manually annotated by BRENDA team
patients with various primary immunodeficiency (PID) syndromes
-
-
Manually annotated by BRENDA team
small and large isozymes SADA and LADA
-
-
Manually annotated by BRENDA team
three enzyme forms ADAI, ADAII and ADAIII
-
-
Manually annotated by BRENDA team
LF 1202
-
-
Manually annotated by BRENDA team
Klebsiella sp. LF 1202
LF 1202
-
-
Manually annotated by BRENDA team
NCIB 8175, soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
NCIB 3251, soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
Micrococcus candidus
NCIB 8610, soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
Micrococcus freudenreichii
ATCC 407, soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
ATCC 9341, soluble enzyme, membrane-bound enzyme; NCIB 9278, soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
Micrococcus sodonensis
ATCC 11880, soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
NC1B 8165, soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
expression in Escherichia coli
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
-
-
Manually annotated by BRENDA team
J-326TK
-
-
Manually annotated by BRENDA team
Nocardioides sp. J-326TK
J-326TK
-
-
Manually annotated by BRENDA team
femal Akkaraman sheep, healthy or with chronic fascioliasis
-
-
Manually annotated by BRENDA team
Pseudomonas iodinum
IFO 3558
-
-
Manually annotated by BRENDA team
albino male Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
male Wistar rats 7-(P7) and 60-day-old (P60)
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
Wistar albino rats
-
-
Manually annotated by BRENDA team
this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; strain 23344c
SwissProt
Manually annotated by BRENDA team
Saccharomyces cerevisiae 23344c
this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; strain 23344c
SwissProt
Manually annotated by BRENDA team
this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; wild-type 972 h
SwissProt
Manually annotated by BRENDA team
Staphylococcus aerogenes
soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
soluble enzyme, membrane-bound enzyme
-
-
Manually annotated by BRENDA team
type small, intermediate and large
-
-
Manually annotated by BRENDA team
Streptomyces sp. J-845S
J-845S
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
apoptosis is enhanced in ADAR1-deficient cells following infection with wild type and V knockout measles virus but not following infection with C knockout measles virus or treatment with tumor necrosis factor-alpha or staurosporine
malfunction
-
disruption of TADA has profound effects on chloroplast translation efficiency, leading to reduced yields of chloroplast-encoded proteins and impaired photosynthetic function
metabolism
-
ADA is a key enzyme in the pathway of hypoxanthine formation
metabolism
Q58936
the enzyme is involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens
physiological function
-
ADAR1 deaminase increases exogenous gene expression at the translational level by decreasing protein kinase regulated by RNA-dependent eIF-2alpha phosphorylation
physiological function
-
ADAR1 is a proviral, antiapoptotic host factor in the context of measles virus infection. ADAR1 enhances the growth of measles virus. ADAR1 suppresses wild type and V knockout measles virus-induced apoptosis. ADAR1 does not protect against tumor necrosis factor-alpha- or staurosporine-induced apoptosis. ADAR1 suppresses wild type and V knockout measles virus-induced protein kinase regulated by RNA activation. ADAR1 suppresses interferon regulatory transcription factor-3 activation by wild type and V knockout measles viruses
physiological function
Q9NZK5
isozyme ADA1 does not induce T cell-dependent differentiation of monocytes into macrophages, isozyme ADA2 but not ADA1 induces T cell-dependent differentiation of monocytes into macrophages and stimulates macrophage proliferation. ADA2 exhibits a growth factor activity and increases proliferation of CD4+ T cells. ADA2 binds to cells via proteoglycans and adenosine receptors
physiological function
-
tRNA adenosine deaminase arginine is a chloroplast tRNA editing protein specifically required for deamination of chloroplast (cp)-tRNAArg(ACG) to cp-tRNAArg(ICG)
physiological function
-
Trypanosoma evansi infection results in either the reduction or increase in the ADA activity in brain of rat. Alterations in ADA activity of the brain in Trypanosoma evansi-infected rats may have implications for pathogenesis of the disease
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-deoxyadenosine + H2O
2',3'-deoxyinosine + NH3
show the reaction diagram
-
1.7% of the activity with adenosine
-
-
?
2',3'-dideoxyadenosine + H2O
2',3'-dideoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2',3'-dideoxyadenosine + H2O
2',3'-dideoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2',3'-isopropylideneadenosine + H2O
2',3'-isopropylideneinosine + NH3
show the reaction diagram
-
-
-
?
2',3'-isopropylideneadenosine + H2O
2',3'-isopropylideneinosine + NH3
show the reaction diagram
Micrococcus sodonensis
-
-
-
?
2',3'-isopropylideneadenosine + H2O
2',3'-isopropylideneinosine + NH3
show the reaction diagram
Nocardioides sp., Nocardioides sp. J-326TK
-
-
-
ir
2',3'-isopropylideneadenosine + H2O
2',3'-isopropylideneinosine + NH3
show the reaction diagram
Streptomyces sp. J-845S
-
-
-
?
2',3'-O-isopropylidenadenosine + H2O
2',3'-O-isopropylideninosine + NH3
show the reaction diagram
-
modeling studies
-
-
?
2',3'-O-isopropylidene adenosine + H2O
2',3'-O-isopropylidene inosine + NH3
show the reaction diagram
-
-
-
-
?
2',3'-O-methoxymethylideneadenosine + H2O
2',3'-O-methoxymethylideneinosine + NH3
show the reaction diagram
-
modeling studies
-
-
?
2'-dAMP + H2O
2'-dIMP + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
-
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
-
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
-
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
Micrococcus sodonensis
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
Q5ZKP6
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
118.9% of the activity with adenosine
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
79.2% of the activity with adenosine
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
82.8% of the activity with adenosine
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
the enzyme plays an important role in the immune system
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
ADAII shows 88% of the activity with adenosine
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
Azotobacter vinelandii 0
-
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
Nocardioides sp. J-326TK
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
Streptomyces sp. J-845S
-
-
-
?
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
Klebsiella sp. LF 1202
-
-
-
?
2'-deoxyguanosine + H2O
2'-deoxyxanthosine + NH3
show the reaction diagram
-
ADAII shows 41% of the activity with adenosine
-
-
?
2,6-diaminopurine arabinoside + H2O
arabinoguanosine + NH3
show the reaction diagram
-
-
-
-
?
2,6-diaminopurine riboside + H2O
guanosine + NH3
show the reaction diagram
-
-
-
-
?
2,6-diaminopurine-2',3'-dideoxyriboside + H2O
2',3'-dideoxyguanosine + NH3
show the reaction diagram
-
-
-
-
?
2,6-diaminopurine-2'-deoxyriboside + H2O
2'-deoxyguanosine + NH3
show the reaction diagram
-
-
-
-
?
2-amino-6-chloropurine riboside + H2O
guanosine + Cl-
show the reaction diagram
Micrococcus sodonensis
-
-
-
?
2-amino-6-chloropurine riboside + H2O
guanosine + ?
show the reaction diagram
-
-
-
-
?
2-amino-6-methoxypurine riboside + H2O
guanosine + ?
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine + H2O
3'-deoxyinosine + NH3
show the reaction diagram
-
-
-
?
3'-deoxyadenosine + H2O
3'-deoxyinosine + NH3
show the reaction diagram
Micrococcus sodonensis
-
-
-
?
3'-deoxyadenosine + H2O
3'-deoxyinosine + NH3
show the reaction diagram
-
2.4% of the activity with adenosine
-
-
?
5'-AMP + H2O
5'-IMP + NH3
show the reaction diagram
-
-
-
-
?
5'-AMP + H2O
5'-IMP + NH3
show the reaction diagram
-
-
-
-
?
5'-AMP + H2O
5'-IMP + NH3
show the reaction diagram
-
-
-
-
ir
5'-AMP + H2O
5'-IMP + NH3
show the reaction diagram
-
1.4% of the activity with adenosine
-
-
?
5'-AMP + H2O
5'-IMP + NH3
show the reaction diagram
Nocardioides sp. J-326TK
-
-
-
-
ir
5'-AMP + H2O
5'-IMP + NH3
show the reaction diagram
Streptomyces sp. J-845S
-
-
-
-
?
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
-
-
-
-
?
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
-
-
-
-
?
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
-
-
-
-
?
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
A5KE01
-
-
-
ir
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
-
-
-
-
?
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
A5KE01
adenosine deaminase has a secondary function in recycling methylthiopurines from the polyamine biosynthetic pathway
-
-
ir
6-chloropurine riboside + H2O
inosine + Cl-
show the reaction diagram
-
-
-
-
?
6-chloropurine riboside + H2O
inosine + Cl-
show the reaction diagram
Micrococcus sodonensis
-
-
-
?
6-chloropurine riboside + H2O
inosine + Cl-
show the reaction diagram
-
-
-
-
-
6-methoxypurinriboside
?
show the reaction diagram
-
-
-
-
?
6-methylaminopurine ribonucleoside + H2O
inosine + methylamine
show the reaction diagram
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
show the reaction diagram
-
ADAII shows 70% of the activity with adenosine
-
-
?
adenine + H2O
purine + NH3
show the reaction diagram
-
-
-
-
?
adenine + H2O
? + NH3
show the reaction diagram
Q8LPL7
-
-
-
?
adenine arabinoside + H2O
inosine arabinoside + NH3
show the reaction diagram
-
-
-
-
?
adenine arabinoside + H2O
inosine arabinoside + NH3
show the reaction diagram
Klebsiella sp., Klebsiella sp. LF 1202
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
P03958
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
-
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Q9NZK5
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
P56658
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
-
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
-
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Micrococcus sodonensis
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
A5KE01
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
Q5ZKP6
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
Q9NZK5
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
preferred substrate
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Q9NZK5
isozyme ADA2 is important in lymphocyte activation, and may be active in sites of inflammation and during hypoxia and in areas of tumor growth with elevated adenosine levels and acidic pH, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
key enzyme in controlling intra- and extracellular adenosine levels
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme activity is correlated with the content in malonaldehyde in nasal polyps and nasal mucosa, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme expression and activity is suppressed in normal pregnancy, but not in woman with recurrent spontaneous abortion
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
P03958
the enzyme is crucial for purine metabolism and normal immune competence
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme is involved in differentiation and maturation of the lymphoid system, the enzyme activity is decreased in multiple sclerosis patients irrespective of whether patients have relapsing-remitting or chronic progressive multiple sclerosis, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme is involved in the bile duct-inflammation and fibrosis caused chronic fascioliasis including an atrophy of the liver parenchyma and liver cirrhosis
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme plays an important role in the immune system
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
adenosine deaminase is involved in purine metabolism and is a key enzyme for the control of the cellular levels of adenosine
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
adenosine deamination is involved in the essential pathway of purine salvage in Plasmodium falciparum, and inhibition of purine salvage results in parasite death
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
metabolization of extracellular adenosine leads to exacerbation of inflammation
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme is important in thymocyte development, especially in late stages, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
detailed quantitative structure-activity relationship, QSAR, nonlinear modeling and analysis, descriptor generation and selection, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
metabolization of extracellular adenosine
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
ADA, nucleotide metabolism
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
P53909
1.4% of the activity of adenine
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Q9P6I7
2.0% of the activity of adenine
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Q8LPL7
30% of the activity of adenine
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
adenosine deaminase markedly enhances the agonist and antagonist affinity and abolishes the negative cooperativity on agonist binding to human striatial adenosine A1 receptors
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Q58936
catalytic efficiency of the enzyme for 5'-deoxyadenosine is 1000fold higher than for S-methyl-5'-thioadenosine, and 10000fold hgher than with S-adenosyl-L-homocysteine or adenosine
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Azotobacter vinelandii 0
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Nocardioides sp. J-326TK
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
Mus musculus C57BL/6
-
the enzyme is important in thymocyte development, especially in late stages, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Streptomyces sp. J-845S
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Klebsiella sp. LF 1202
-
-
-
ir
adenosine 3'-monophosphate + H2O
inosine 3'-monophosphate + NH3
show the reaction diagram
Q8LPL7
56% of the activity of adenine
-
-
?
adenosine 3'-phosphate + H2O
inosine 3'-phosphate + NH3
show the reaction diagram
P53909
3.3% of the activity of adenine
-
-
?
adenosine 3'-phosphate + H2O
inosine 3'-phosphate + NH3
show the reaction diagram
Q9P6I7
4.5% of the activity of adenine
-
-
?
adenosine-3'-monophosphate + H2O
IMP-3'-monophosphate + NH3
show the reaction diagram
-
-
-
-
?
adenosine-3'-monophosphate + H2O
IMP-3'-monophosphate + NH3
show the reaction diagram
-
-
-
-
ir
adenosine-3'-monophosphate + H2O
IMP-3'-monophosphate + NH3
show the reaction diagram
Streptomyces sp. J-845S
-
-
-
-
?
ADP + H2O
5'-IDP + NH3
show the reaction diagram
-
-
-
-
?
ADP + H2O
5'-IDP + NH3
show the reaction diagram
Nocardioides sp., Nocardioides sp. J-326TK
-
-
-
-
ir
arabinoadenosine + H2O
arabinoinosine + NH3
show the reaction diagram
-
-
-
-
?
ATP + H2O
ITP + NH3
show the reaction diagram
-
-
-
-
?
ATP + H2O
ITP + NH3
show the reaction diagram
-
-
-
-
ir
ATP + H2O
ITP + NH3
show the reaction diagram
Q9P6I7
3.3% of the activity of adenine
-
-
?
ATP + H2O
ITP + NH3
show the reaction diagram
P53909
3.3% of the activity of adenine
-
-
?
ATP + H2O
ITP + NH3
show the reaction diagram
Q8LPL7
68% of the activity of adenine
-
-
?
cAMP + H2O
?
show the reaction diagram
-
-
-
-
?
double-stranded RNA + H2O
?
show the reaction diagram
-
-
-
-
?
formycin A + H2O
2-(hydroxymethyl)-5-(1H-pyrazolo[4,3-d]pyrimidin-3-yl)tetrahydrofuran-3,4-diol + NH3
show the reaction diagram
Azotobacter vinelandii, Azotobacter vinelandii 0
-
-
-
-
?
guanine + H2O
xanthine + NH3
show the reaction diagram
-
ADAII shows 47% of the activity with adenosine
-
-
?
N6-isopentenyladenosine + H2O
?
show the reaction diagram
Q9P6I7
1.9% of the activity of adenine
-
-
?
N6-isopentenyladenosine + H2O
inosine + 3-methylbut-3-en-1-amine
show the reaction diagram
P53909
1.2% of the activity of adenine
-
-
?
viramidine + H2O
ribavirin + NH3
show the reaction diagram
-
the enzyme catalyzes viramidine deamination to ribavirin in vivo as a prodrug conversion to active drug, pharmacokinetic analysis, overview, substrate and product determination by LC-MS
-
-
?
guanosine + H2O
xanthosine + NH3
show the reaction diagram
-
ADAII shows 75% of the activity with adenosine
-
-
?
additional information
?
-
-
regulatory role of dipeptidyl peptidase IV on adenosine deaminase activity
-
-
-
additional information
?
-
-
the enzyme is involved in purine metabolism and plays an important role in the mechanism of the immune system
-
-
-
additional information
?
-
-
activity with 3'-deoxyadenosine, 2',3'-deoxyadenosine and 2'-O-methyladenosine is less than 0.5% of the activity with adenine
-
-
-
additional information
?
-
-
no activity with 2'-O-methyladenosine
-
-
-
additional information
?
-
-
no activity with 3'-deoxyadenosine, 2',3'-deoxyadenosine and 2'-O-methyladenosine
-
-
-
additional information
?
-
-
the enzyme may facilitate catalysis via the formation of a stabilizing conformation at the reaction transition state
-
-
-
additional information
?
-
-
the enzyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview
-
-
-
additional information
?
-
-
the large isozyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview, substrate specificities of the large and the small isozyme, overview
-
-
-
additional information
?
-
-
substrate specificities of the large and the small isozyme, overview
-
-
-
additional information
?
-
-
adenosine deaminase inhibition enhances the efficiency of signal transduction and inotropic response mediated by A1 adenosine receptor in hyperthyroid guinea pig atrium via elevated intracellular adenosine levels, overview
-
-
-
additional information
?
-
-
adenosine modulates the immune system and inhibits inflammation via reduction of cytokine biosynthesis and neutrophil functions, enzyme inhibition reduces inflammation in experimental colitis, overview
-
-
-
additional information
?
-
-
no activity with 5'-methylthioadenosine
-
-
-
additional information
?
-
Q8LPL7
does not cleave cytokinins
-
-
-
additional information
?
-
-
ADA is associated with the Galphai-3 protein-coupled A1 receptor
-
-
-
additional information
?
-
-
Plasmodium gallinaceum ADA does not have significant activity for 5'-methylthioadenosine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
Q5ZKP6
-
-
-
ir
2'-deoxyadenosine + H2O
2'-deoxyinosine + NH3
show the reaction diagram
-
the enzyme plays an important role in the immune system
-
-
?
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
-
-
-
-
?
5'-methylthioadenosine + H2O
5'-methylthioinosine + NH3
show the reaction diagram
A5KE01
adenosine deaminase has a secondary function in recycling methylthiopurines from the polyamine biosynthetic pathway
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
P56658
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
A5KE01
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
Q5ZKP6
-
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
Q9NZK5
isozyme ADA2 is important in lymphocyte activation, and may be active in sites of inflammation and during hypoxia and in areas of tumor growth with elevated adenosine levels and acidic pH, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
key enzyme in controlling intra- and extracellular adenosine levels
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme activity is correlated with the content in malonaldehyde in nasal polyps and nasal mucosa, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme expression and activity is suppressed in normal pregnancy, but not in woman with recurrent spontaneous abortion
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
P03958
the enzyme is crucial for purine metabolism and normal immune competence
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme is involved in differentiation and maturation of the lymphoid system, the enzyme activity is decreased in multiple sclerosis patients irrespective of whether patients have relapsing-remitting or chronic progressive multiple sclerosis, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme is involved in the bile duct-inflammation and fibrosis caused chronic fascioliasis including an atrophy of the liver parenchyma and liver cirrhosis
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme plays an important role in the immune system
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
adenosine deaminase is involved in purine metabolism and is a key enzyme for the control of the cellular levels of adenosine
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
adenosine deamination is involved in the essential pathway of purine salvage in Plasmodium falciparum, and inhibition of purine salvage results in parasite death
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
metabolization of extracellular adenosine leads to exacerbation of inflammation
-
-
ir
adenosine + H2O
inosine + NH3
show the reaction diagram
-
the enzyme is important in thymocyte development, especially in late stages, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
ADA, nucleotide metabolism
-
-
ir
viramidine + H2O
ribavirin + NH3
show the reaction diagram
-
the enzyme catalyzes viramidine deamination to ribavirin in vivo as a prodrug conversion to active drug, pharmacokinetic analysis, overview
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
Mus musculus C57BL/6
-
the enzyme is important in thymocyte development, especially in late stages, overview
-
-
?
additional information
?
-
-
regulatory role of dipeptidyl peptidase IV on adenosine deaminase activity
-
-
-
additional information
?
-
-
the enzyme is involved in purine metabolism and plays an important role in the mechanism of the immune system
-
-
-
additional information
?
-
-
the enzyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview
-
-
-
additional information
?
-
-
the large isozyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview
-
-
-
additional information
?
-
-
adenosine deaminase inhibition enhances the efficiency of signal transduction and inotropic response mediated by A1 adenosine receptor in hyperthyroid guinea pig atrium via elevated intracellular adenosine levels, overview
-
-
-
additional information
?
-
-
adenosine modulates the immune system and inhibits inflammation via reduction of cytokine biosynthesis and neutrophil functions, enzyme inhibition reduces inflammation in experimental colitis, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Q58936
the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal
lithium ion
-
-
Mg2+
-
91% inhibition
Mg2+
-
activates
Ni2+
Q58936
the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal
Zn2+
-
coordinated by His15, His17, His214, and Asp295
Zn2+
-
zinc-metalloenzyme, binding structure
Zn2+
A5KE01
-
Zn2+
-
ADA contains a tightly bound Zn2+ which is required for activity, the stability of the protein is decreased significantly in the absence of Zn2+
Zn2+
Q58936
the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal
additional information
-
no effect on activity of Ca2+, Mg2+
additional information
-
there are no significant changes in ADA activity in the presence of cadmium acetate, potassium dichromate, lead acetate, zinc chloride, copper sulfate, cobalt chloride, and manganese chloride
additional information
Q58936
metal-dependent enzyme. The metal can not be removed from the enzyme nor can the nature of the catalytic metal be established. Dialysis of the wild-type enzyme for 3 days against a dipicolinic acid containing buffer fails to show any lowering enzyme activity, again indicating that chelation is not able to remove the bound metal in the active site. The catalytic metal is not removed by EDTA
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+)-erythro-9-(2-hydroxy-3-nonyl)adenine
Q9NZK5
specific inhibitor of ADA1
(S)-(-)-epoxydecane
-
-
1,6-Dihydro-6-(hydroxymethyl)purine riboside
-
-
1-(4-benzylphenyl)-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
-
1-(4-fluorophenyl)-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
-
1-(4-methoxyphenyl)-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
-
1-(hydroxymethyl)nonyl methanesulfonate
-
-
1-(tert-butyldimethylsilanyloxy)-decan-2-ol
-
-
1-(tert-butyldimethylsilanyloxymethyl)nonyl methanesulfonate
-
-
1-alkyl-3-amino-4-pyrazolecarbonitriles
-
-
1-alkyl-4-aminopyrazolo[3,4d]pyrimidines
-
-
1-benzyl-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
-
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
i.e. 1-dEHNA
1-deazaadenosine
-
-
1-deazaadenosine
-
-
1-methyl adenosine
-
-
1-[(1R)-1-(hydroxymethyl)-3-(6-[[(1-methyl-1H-benzimidazol-2-yl)acetyl]amino]-1H-indol-1-yl)propyl]-1H-imidazole-4-carboxamide
-
-
1-[(1R)-1-(hydroxymethyl)-3-naphthalen-1-ylpropyl]-1H-imidazole-4-carboxamide
-
-
1-[(1R)-1-(hydroxymethyl)-3-phenylpropyl]-1H-imidazole-4-carboxamide
-
-
1-[(1R)-2-hydroxy-1-phenylethyl]-1H-imidazole-4-carboxamide
-
-
1-[(1R)-3-[6-(acetylamino)-1H-indol-1-yl]-1-(hydroxymethyl)propyl]-1H-imidazole-4-carboxamide
-
-
1-[(1R)-3-[6-(hexanoylamino)-1H-indol-1-yl]-1-(hydroxymethyl)propyl]-1H-imidazole-4-carboxamide
-
-
1-[(1R,2S)-2-hydroxy-1-(2-naphthalen-1-ylethyl)propyl]-1H-imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(1-methyl-5-(3-phenylpropoxy)indol-3-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(5-(3-phenylpropoxy)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(3-(1-methylbenzimidazol-2-yl)propionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(3-(3-pyridyl)propionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(3-(4-methoxyphenyl)propionylamino)indol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(3-(4-methylphenyl)propionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(3-phenylpropionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(3-phenylpropoxy)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(4-(4-methylphenyl)butyrylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(4-phenylbutoxy)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(4-phenylbutyrylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(5-phenylvalerylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-1-hydroxy-4-(6-(6-phenylhexanoylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(5-(3-(4-chlorophenyl)propoxyl)-1-methylindol-3-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(5-hexyloxy-1-methylindol-3-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(5-hexyloxyindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(6-(3-(4-chlorophenyl)propoxy)indol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(6-(3-benzylureido)indol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(6-acetylaminoindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(6-butoxyindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(6-hexanoylaminoindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[(R)-4-(6-hexyloxyindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
1-[4-(dimethylamino)phenyl]-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
-
1-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-3-(4-phenoxyphenyl)urea
-
-
1-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-3-phenylurea
-
-
1-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-3-[4-(trifluoromethyl)phenyl]urea
-
-
2',3'-o-isopropyliden adenosine
-
-
2'-deoxy-4-amino-1-(beta-D-ribofuranosyl)-3H-imidazo[4,5-d]pyridazin-7-(6H)-one
-
-
2'-deoxy-7-amino-1-(beta-D-ribofuranosyl)-1H-imidazo[4,5-d]pyridazin-4-(5H)-one
-
-
2'-Deoxycoformycin
-
0.0015 nM, 50% inhibition
2'-Deoxycoformycin
-
0.0004 nM, 50% inhibition
2'-Deoxycoformycin
-
0.15 nM, 50% inhibition
2'-Deoxycoformycin
-
a natural product transition state analogue inhibitor
2'-Deoxycoformycin
-
-
2'-Deoxycoformycin
A5KE01
-
2'-Deoxycoformycin
-
-
2'-deoxyformycin
-
50% inhibition of isozyme ADA2 at 0.09 mM
2,6-Diaminopurine
-
-
2,6-diaminopurine sulfate
-
-
2-alkyl-4-aminopyrazolo[3,4d]pyrimidines
-
-
2-Amino-4-hydroxypteridine
-
-
2-Aminopurine
-
-
2-Aminopurine
-
competitive inhibition
2-Deoxycoformycin
-
-
2-mercaptoethanol
-
-
3-amino-1-(2-hydroxy-3-nonyl)4-pyrazolecarbonitrile
-
-
3-amino-1-(2-keto-3-nonyl)4-pyrazolecarbonitrile
-
-
3-amino-1-beta-hydroxyalkyl-4-pyrazolecarbonitriles
-
-
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
i.e. 3-dEHNA
3-deazaadenosine
-
-
4-acetylamino-2-decylpyrazolo[3,4d]pyrimidine
-
-
4-amino-1-(2-hydroxy-3-nonyl)pyrazolo[3,4d]pyrimidine
-
-
4-amino-1-(beta-D-ribofuranosyl)-3H-imidazo[4,5-d]pyridazin-7-(6H)-one
-
-
4-amino-1-(beta-hydroxyethyl)pyrazolo[3,4d]pyrimidine
-
-
4-amino-1-(beta-hydroxyoctyl)pyrazolo[3,4d]pyrimidine
-
-
4-amino-2-(2-hydroxy-1-decyl)pyrazole[3,4-d]pyrimidine
-
i.e. APP
4-amino-2-decyl-6-phenylpyrazolo[3,4d]pyrimidine
-
-
4-amino-5-imidazole carboxamide-HCl
-
-
4-amino-5-imidazole carboxyamide ribonucleoside
-
-
-
4-aminopyridine
-
competitive inhibition
4-aminopyrimidine
-
competitive inhibition
4-fluoro-N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]benzamide
-
-
4-hydroxypyridine
-
competitive inhibition
4-methoxy-N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]benzamide
-
-
5'-methylthio-2'-deoxycoformycin
-
-
5'-methylthiocoformycin
-
-
5'-methylthiocoformycin
-
specific transition state analogue inhibitor of Plasmodium ADA
5'-methylthiocoformycin
-
specific transition state analogue inhibitor of Plasmodium ADA, 5'-methylthiocoformycin is a subnanomolar inhibitor of Plasmodium falciparum ADA and demonstrates more than 20000fold selectivity relative to human ADA
5'-methylthiocoformycin
-
poor inhibitor of Plasmodium gallinaceum ADA
5'-methylthiocoformycin
-
specific transition state analogue inhibitor of Plasmodium ADA
5-carbamimidoyl-1-(beta-D-ribofuranosyl)-1H-imidazole-4-carbohydrazide
-
-
6-Chloropurine
-
-
6-fluoro-D,L-tryptophan
-
adenosine analogue, induces conformational changes inhibiting the enzyme, structural mechanism, overview
6-methylamino riboside
-
-
6-Methylmercaptopurine riboside
-
-
7,8-dihydro-1-p-methoxybenzyl-6H-6,7-dimethyl-8-propoxyimidazo[4,5-e][1,2,4]-triazepine
-
-
7,8-dihydro-1-p-methoxybenzyl-8-(2-methoxyethoxy)-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
-
7,8-dihydro-1-p-methoxybenzyl-8-(3-methylbenzyloxy)-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
-
7,8-dihydro-1-p-methoxybenzyl-8-(4-methoxybenzyloxy)-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
-
7,8-dihydro-8-ethoxy-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
-
7,8-dihydro-8-isopropoxy-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
-
7,8-dihydro-8-methoxy-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
-
7-amino-1-(beta-D-ribofuranosyl)-1H-imidazo[4,5-d]pyridazin-4-(5H)-one
-
-
8-Bromoadenosine
-
-
8-butoxy-7,8-dihydro-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
-
9-(p-aminobenzyl)adenine
-
-
acetaminophen
-
competitive at 27C, uncompetitive at 37C
Acetylsalicylic acid
-
-
adenine
-
competitive inhibition
adenosine analogs
-
-
-
adenosine analogues
-
induce conformational changes inhibiting the enzyme, structural mechanism, overview
-
adenosine monosulfate
-
-
Ag+
Micrococcus sodonensis
-
-
aminoxyacetic acid
Micrococcus sodonensis
-
-
aspirin
-
significantly decreases adenosine deaminase activity, the half-life of adenosine will be lengthened as a consequence of decreased adenosine deaminase activity
aza adenosine analogues
-
-
-
Ba2+
-
44% inhibition at 5 mM
basic histone proteins
-
-
-
Ca2+
-
69% inhibition at 5 mM
Ca2+
-
strong inhibition
caffeic acid phenethyl ester
-
reduces the enzyme activity in vivo, suppresses the induction of the enzyme by cisplatin in vivo
Caffeine
-
-
coformycin
-
-
coformycin
-
-
coformycin
-
-
coformycin
-
a natural product transition state analogue inhibitor
coformycin
Q9NZK5
complete inhibition of ADA2 at 1 mM
corticosterone
-
-
corticosterone
Q5ZKP6
inhibits the adenosine deaminase activity in an age- and region-specific manner
Cu2+
-
-
Cu2+
-
0.029 mM, 50% inhibition
Cu2+
-
81% inhibition at 5 mM
deaza adenosine analogues
-
-
-
Deoxycoformycin
-
-
diclofenac
-
competitive at 27C, uncompetitive at 37C
diethyl dicarbonate
-
-
dodecyltrimethylammonium bromide
-
at low concentrations (approximately 0.1 mM), enzyme activity decreases, it increases at a slightly higher concentration (about 0.2 mM), and finally decreases again (about 0.3 mM), CD spectra of adenosine deaminase and DTAB, determination of helix content, MD calculations
EDTA
Micrococcus sodonensis
-
-
erythro-9-(2-hydroxy-3-nonly)adenine
-
-
-
erythro-9-(2-hydroxy-3-nonyl)-3-deazaadenine
-
-
erythro-9-(2-hydroxy-3-nonyl)-adenine
-
-
erythro-9-(2-hydroxy-3-nonyl)-adenine
-
0.1 mM concentration, ADA2 is practically unaffected; 0.1 mM concentration, complete and reversible inhibition
erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride
-
0.4 nM, 50% inhibition
erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride
-
0.45 nM, 50% inhibition
erythro-9-(2-hydroxy-3-nonyl)adenine
-
-
erythro-9-(2-hydroxy-3-nonyl)adenine
-
i.e. EHNA, specific for isozyme ADA1, no inhibition of isozyme ADA2
erythro-9-(2-hydroxy-3-nonyl)adenine
-
i.e. EHNA, specific for isozyme ADA1
erythro-9-(2-hydroxy-3-nonyl)adenine
-
i.e. EHNA
erythro-9-(2-hydroxy-3-nonyl)adenine
-
i.e. EHNA
erythro-9-(2-hydroxy-3-nonyl)adenine
-
i.e. (+)-EHNA
erythro-9-(2-hydroxy-3-nonyl)adenine
-
-
erythro-9-(2-hydroxy-3-nonyl)adenine
-
EHNA 0.1mM
erythro-9-(2-hydroxy-3-nonyl)adenine
-
-
erythro-9-(2-hydroxy-3-nonyl)adenine
-
ADA-specific inhibitor, almost complete inhibition at 0.00005 mM
erythro-9-(2-hydroxy-3-nonyl)adenine
-
-
erythro-9-(2-hydroxy-3-nonyl)adenine
-
-
erythro-9-(2-hydroxy-3-nonyl)adenine hydrochloride
-
i.e. EHNA
erythro-9-(2-hydroxy-3-nonyl)adenine hydrochloride
-
-
ethanol
-
CD spectra of adenosine deaminase and EtOH, determination of helix content, MD calculations
FR234938
-
i.e. 1-((1R,2S)-2-hydroxy-1-(2-(1-naphthyl)ethyl)propyl)1H-imidazole-4-carboxamine, a non-nucleoside inhibitor, the inhibition is blocked by an A2a adenosine receptor antagonist
guanosine
-
-
heparin
-
-
Hg2+
Micrococcus sodonensis
-
-
Hg2+
-
-
Hg2+
-
88% inhibition at 5 mM
HgCl2
-
inhibition of ADA activity is observed in the soluble fraction at 0.005-0.25 mM HgCl2 (84.6-92.6% respectively), whereas inhibition occurs at 0.005-0.25 mM in membrane fractions (20.9-26% respectively). The inhibition is partially or fully abolished by 0.5 mM dithiothreitol or EDTA
IgG
-
anti adenosine deaminase
-
indole-3-acetic acid
-
the plant hormone decreases enzyme activity in hearts, but not in kidney and muscle
Inosine
-
competitive inhibition
iodoacetamide
Micrococcus sodonensis
-
-
iodoacetic acid
Micrococcus sodonensis
-
-
iodoacetic acid
-
-
Iodopurine
-
-
isosorbide mononitrate
-
significantly decreases adenosine deaminase activity, the half-life of adenosine will be lengthened as a consequence of decreased adenosine deaminase activity
kinetin
-
the plant hormone decreases enzyme activity in hearts, but not in kidney and muscle
L-cysteine
-
-
Lysozyme
-
-
-
methanol
-
MD calculations, discussion of effects
methylmercury
-
concentration 0.1, 1.0, 20 M, inhibits adenosine deaminase activity in 7- and 60-day-old rats in a concentration-dependent manner, glutathione (50 microM) and dithiothreitol (50 microM) prevent the reduction of adenosine deaminase activity both in cerebral cortex and hippocampus, garlic alcoholic extract (100 microg/ml) abolishes the reduction of adenosine deaminase
metoprolol
-
significantly decreases adenosine deaminase activity, the half-life of adenosine will be lengthened as a consequence of decreased adenosine deaminase activity
Mg2+
-
0.055 mM, 50% inhibition
Mg2+
-
strong inhibition
Mn2+
-
0.046 mM, 50% inhibition
N-ethylmaleimide
Micrococcus sodonensis
-
-
N-ethylmaleimide
-
-
N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-2-phenylacetamide
-
-
N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-4-(trifluoromethyl)benzamide
-
-
N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]benzamide
-
-
N6-methyladenosine
-
-
Na2SO4
-
CD spectra of adenosine deaminase and Na2SO4, determination of helix content, MD calculations, discussion of effects
NaCl
-
MD calculations, discussion of effects
Ni2+
-
61% inhibition at 5 mM
o-phenanthroline
-
-
o-phenanthroline
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
Micrococcus sodonensis
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuryphenylsulfonate
-
-
p-hydroxymercuribenzoic acid
-
-
Pb2+
Micrococcus sodonensis
-
-
Pentachlorophenol
-
-
pentostatin
-
-
pentostatin
-
i.e. (8R)-3-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-3,6,7,8-tetrahydroimidazo[4,5-d][1,3]diazepin-8-ol
pentostatin
-
i.e. 2'-deoxycoformycin, a selective ADA inhibitor
phenylhydrazine
-
competitive inhibition
progesterone
-
progesterone decreases adenosine deaminase levels, adenosine deaminase levels show regional specificity with differences among the cerebral hemispheres, cerebellum, and brainstems structures
purine
-
competitive inhibition
purine riboside
-
-
purine riboside
-
-
purine riboside
-
-
purine riboside
-
adenosine analogue, induces conformational changes inhibiting the enzyme, structural mechanism, overview
purine riboside
-
-
reduced glutathione
-
-
simvastatin
-
inhibits ADA1 activity by 46.7%; significantly decreases adenosine deaminase activity, the half-life of adenosine will be lengthened as a consequence of decreased ADA activity
Sodium dodecyl sulfate
-
at low concentrations (approximately 0.1 mM) enzyme activity decreases, it increases at a slightly higher concentration (about 0.4 mM), and finally decreases again (about 0.55 mM), CD spectra of adenosine deaminase and SDS, determination of helix content, MD calculations
Sodium selenate
-
does not alter enzyme activity
spermidine
-
-
Theobromine
-
-
theophylline
-
-
theophylline
-
-
theophylline
-
-
thiol reagents
-
-
Zn2+
Micrococcus sodonensis
-
-
Zn2+
-
65% inhibition at 5 mM
Zn2+
-
decrease of 31.3% on adenosine deamination in membranes is observed in the presence of 5 mM Zn2+
Mn2+
-
85% inhibition at 5 mM
additional information
-
no inhibition by erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride
-
additional information
-
structure-based design, synthesis, and structure-activity relationship studies of non-nucleoside adenosine deaminase inhibitors, overview
-
additional information
-
molecular dynamic simulations of inhibitor-enzyme interaction, overview
-
additional information
-
loss of activity precedes the global secondary and tertiary structure transition when the enzyme is exposed to denaturant, structural mechanism, overview
-
additional information
-
no inhibition of the bovine enzyme by 5'-methylthiocoformycin and 5'-methylthio-2'-deoxycoformycin
-
additional information
-
no inhibition of the human enzyme by 5'-methylthiocoformycin and 5'-methylthio-2'-deoxycoformycin
-
additional information
-
cytotoxic effects of inhibitors against human breast cancer cell lines MCF-7 and MCF-10A
-
additional information
-
ADA1 activity is not changed significantly by aspirin, metoprolol, isosorbide mononitrate, and molsidominemolsidomine; molsidomine does not influence plasma adenosine deaminase activity significantly
-
additional information
-
the trend of relative activity is directly proportional to helicity and inversely proportional to accessible surface area
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8-Azaadenine
-
-
adenine
-
-
cisplatin
-
cisplatin intoxification increases the enzyme activity in vivo, supressable by caffeic acid phenethyl ester
dibutyryl-cAMP
-
-
dibutyryl-cAMP
Q5ZKP6
causes a significant increase in the activity level of adenosine deaminase depending on the regions of the gastrointestinal tract
EDTA
Q58936
stimulates
Inosine
-
-
N-acetyl-D-glucosamine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.29
2',3'-isopropylidene adenosine
-
-
0.004
2'-deoxyadenosine
-
-
0.016
2'-deoxyadenosine
-
-
0.034
2'-deoxyadenosine
-
-
0.034
2'-deoxyadenosine
-
pH 7.4, 37C
0.039
2'-deoxyadenosine
-
kidney cortex large isozyme
0.04
2'-deoxyadenosine
-
-
0.04
2'-deoxyadenosine
-
pleural fluid large isozyme
0.042
2'-deoxyadenosine
-
blood serum large isozyme
0.05 - 0.07
2'-deoxyadenosine
-
-
0.052
2'-deoxyadenosine
-
-
0.053
2'-deoxyadenosine
-
-
0.057
2'-deoxyadenosine
-
-
0.135
2'-deoxyadenosine
-
spleen small isozyme
0.189
2'-deoxyadenosine
-
lung small isozyme
0.2
2'-deoxyadenosine
-
-
0.22
2'-deoxyadenosine
-
-
0.333
2'-deoxyadenosine
-
-
0.4
2'-deoxyadenosine
-
-
0.56
2'-deoxyadenosine
-
37C, pH not specified in the publication
0.0044
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0087
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0095
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.022
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.115
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.31
6-Chloropurine riboside
-
-
0.277
6-chloropurinriboside
-
-
0.081
6-methoxypurinriboside
-
-
0.032
adenine
Q9P6I7
-
0.000023
adenosine
-
-
0.000046
adenosine
-
-
0.0147
adenosine
-
pH 7.5, 25C
0.022
adenosine
-
-
0.023
adenosine
-
-
0.026
adenosine
-
-
0.026
adenosine
-
25C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM
0.028
adenosine
-
type 2
0.029
adenosine
-
type 2-1
0.029
adenosine
-
37C, pH not specified in the publication
0.031
adenosine
-
type 1
0.032
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.033
adenosine
-
-
0.037
adenosine
-
-
0.0385
adenosine
-
-
0.041
adenosine
-
pH 7.4, 37C
0.048
adenosine
-
-
0.049
adenosine
-
-
0.05
adenosine
-
-
0.05
adenosine
-
-
0.051
adenosine
-
-
0.053
adenosine
-
blood serum large isozyme
0.055 - 0.07
adenosine
-
-
0.056
adenosine
-
kidney cortex large isozyme
0.057
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.06
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.066
adenosine
-
El
0.07
adenosine
-
pleural fluid large isozyme
0.071
adenosine
-
wild-type, pH 7.5, 37C
0.072
adenosine
Q58936
pH and temperature not specified in the publication, mutant enzyme Y136R
0.074
adenosine
-
Es
0.075
adenosine
-
-
0.083
adenosine
-
mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.087
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.088
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.093
adenosine
-
spleen small isozyme
0.104
adenosine
-
mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.12
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.129
adenosine
-
lung small isozyme
0.144
adenosine
-
-
0.15
adenosine
Q58936
pH and temperature not specified in the publication, mutant enzyme E150R; pH and temperature not specified in the publication, wild-type enzyme
0.19
adenosine
-
membrane-bound ADA
0.22
adenosine
-
-
0.22
adenosine
-
soluble fraction
0.5
adenosine
-
pH 7.5, 37C, ADAII
0.67
adenosine
-
-
0.715
adenosine
-
-
0.734
adenosine
-
pH 6.8, 37C
1
adenosine
-
pH 7.2, 37C, ADAII
1.13
adenosine
-
pH 7.5, 37C, Vmax is 2.61 nmol NH3/min/mg protein
2.15
adenosine
-
-
6.6
adenosine
-
-
2
Adenosine-3'-monophosphate
-
-
0.17
AMP
-
-
0.5
cAMP
-
-
additional information
additional information
-
kinetics of the large and the small isozyme
-
additional information
additional information
-
isozymes ADA1 and ADA2 are kinetically distinct
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
optimization of a spectrometric kinetic measurement method pure enzyme, kinetics in presence of substrate, product and inhibtior, overview
-
additional information
additional information
-
optimization of a spectrometric kinetic measurement method using Hut78 cell extract, kinetics in presence of substrate, product and inhibtior, overview
-
additional information
additional information
-
thermodynamics and kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.13
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.31
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.35
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.51
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
5.8
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.005
adenosine
Q8LPL7
-
1.8
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
1.9
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
4.7
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
5.3
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
5.6
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
6.8
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
11.5
adenosine
-
37C, pH not specified in the publication
12.9
adenosine
-
mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
14
adenosine
-
mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
18.5
adenosine
-
pH 7.5, 25C
110
adenosine
Q58936
pH and temperature not specified in the publication, mutant enzyme E150R; pH and temperature not specified in the publication, wild-type enzyme
140
adenosine
Q58936
pH and temperature not specified in the publication, mutant enzyme Y136R
180
adenosine
-
25C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
14
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
400
23
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
400
36
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
400
50
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
400
79
5'-methylthioadenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
400
30
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
57
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
59
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
61
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
64
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
82
adenosine
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
130
adenosine
-
mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
150
adenosine
-
mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
122
750
adenosine
Q58936
pH and temperature not specified in the publication, mutant enzyme E150R; pH and temperature not specified in the publication, wild-type enzyme
122
2900
adenosine
Q58936
pH and temperature not specified in the publication, mutant enzyme Y136R
122
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00186
1-(4-benzylphenyl)-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
30C, pH 7.12
0.0000115
1-(4-fluorophenyl)-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
30C, pH 7.9
0.0002827
1-(4-methoxyphenyl)-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
30C, pH 7.8
0.000857
1-benzyl-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
30C, pH 7.14
0.0004
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
kidney cortex large isozyme
0.00045
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
pleural fluid large isozyme
0.0006
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
blood serum large isozyme
0.0011
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
spleen small isozyme
0.0012
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
lung small isozyme
0.0016
1-deazaadenosine
-
kidney cortex large isozyme
0.0025
1-deazaadenosine
-
pleural fluid large isozyme
0.0029
1-deazaadenosine
-
lung small isozyme
0.0029
1-deazaadenosine
-
blood serum large isozyme
0.0057
1-deazaadenosine
-
spleen small isozyme
0.007
1-deazaadenosine
-
pH 7.1, 23C
0.0074
1-deazaadenosine
-
pH 7.1, 23C, after preincubation with diethyl dicarbonate
0.000077
1-[(1R)-1-(hydroxymethyl)-3-(6-[[(1-methyl-1H-benzimidazol-2-yl)acetyl]amino]-1H-indol-1-yl)propyl]-1H-imidazole-4-carboxamide
-
-
0.0068
1-[(1R)-1-(hydroxymethyl)-3-naphthalen-1-ylpropyl]-1H-imidazole-4-carboxamide
-
-
0.059
1-[(1R)-1-(hydroxymethyl)-3-phenylpropyl]-1H-imidazole-4-carboxamide
-
-
0.54
1-[(1R)-2-hydroxy-1-phenylethyl]-1H-imidazole-4-carboxamide
-
-
0.013
1-[(1R)-3-[6-(acetylamino)-1H-indol-1-yl]-1-(hydroxymethyl)propyl]-1H-imidazole-4-carboxamide
-
-
0.00024
1-[(1R)-3-[6-(hexanoylamino)-1H-indol-1-yl]-1-(hydroxymethyl)propyl]-1H-imidazole-4-carboxamide
-
-
0.00011
1-[(1R,2S)-2-hydroxy-1-(2-naphthalen-1-ylethyl)propyl]-1H-imidazole-4-carboxamide
-
-
0.000026
1-[(R)-1-hydroxy-4-(1-methyl-5-(3-phenylpropoxy)indol-3-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.0019
1-[(R)-1-hydroxy-4-(5-(3-phenylpropoxy)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000011
1-[(R)-1-hydroxy-4-(6-(3-(1-methylbenzimidazol-2-yl)propionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000017
1-[(R)-1-hydroxy-4-(6-(3-(3-pyridyl)propionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000057
1-[(R)-1-hydroxy-4-(6-(3-(4-methoxyphenyl)propionylamino)indol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.000038
1-[(R)-1-hydroxy-4-(6-(3-(4-methylphenyl)propionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.00003
1-[(R)-1-hydroxy-4-(6-(3-phenylpropionylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000017
1-[(R)-1-hydroxy-4-(6-(3-phenylpropoxy)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000034
1-[(R)-1-hydroxy-4-(6-(4-(4-methylphenyl)butyrylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000012
1-[(R)-1-hydroxy-4-(6-(4-phenylbutoxy)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000016
1-[(R)-1-hydroxy-4-(6-(4-phenylbutyrylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.0000077
1-[(R)-1-hydroxy-4-(6-(5-phenylvalerylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.000091
1-[(R)-1-hydroxy-4-(6-(6-phenylhexanoylamino)indol-1-yl)-2-butyl]imidazole-4-carboxamide
-
-
0.0000049
1-[(R)-4-(5-(3-(4-chlorophenyl)propoxyl)-1-methylindol-3-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.000013
1-[(R)-4-(5-hexyloxy-1-methylindol-3-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.0062
1-[(R)-4-(5-hexyloxyindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.000013
1-[(R)-4-(6-(3-(4-chlorophenyl)propoxy)indol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.0000075
1-[(R)-4-(6-(3-benzylureido)indol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.0013
1-[(R)-4-(6-acetylaminoindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.00024
1-[(R)-4-(6-butoxyindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.000024
1-[(R)-4-(6-hexanoylaminoindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.000055
1-[(R)-4-(6-hexyloxyindol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide
-
-
0.0000201
1-[4-(dimethylamino)phenyl]-3-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]urea
-
30C, pH 7.11
0.00108
1-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-3-(4-phenoxyphenyl)urea
-
30C, pH 7.13
0.0000242
1-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-3-phenylurea
-
30C, pH 7.7
0.0000016
1-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-3-[4-(trifluoromethyl)phenyl]urea
-
30C, pH 7.10
0.0121
2'-deoxy-4-amino-1-(beta-D-ribofuranosyl)-3H-imidazo[4,5-d]pyridazin-7-(6H)-one
-
pH 7.4, 25C
0.052
2'-deoxy-7-amino-1-(beta-D-ribofuranosyl)-1H-imidazo[4,5-d]pyridazin-4-(5H)-one
-
pH 7.4, 25C
0.0000034
2'-Deoxycoformycin
-
37C, pH not specified in the publication
0.33
2-Aminopurine
-
pH 7.5, 25C
0.000025
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
blood serum large isozyme
0.000036
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
pleural fluid large isozyme
0.00005
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
kidney cortex large isozyme
0.00011
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
spleen small isozyme
0.00012
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine
-
lung small isozyme
0.52
3-deazaadenosine
-
blood serum large isozyme
0.69
3-deazaadenosine
-
kidney cortex large isozyme
0.81
3-deazaadenosine
-
lung small isozyme
1
3-deazaadenosine
-
above, spleen small isozyme
1
3-deazaadenosine
-
above, pleural fluid large isozyme
0.000066
4-amino-1-(2-hydroxy-3-nonyl)pyrazolo[3,4d]pyrimidine
-
pH 7.2, 30C
0.0106
4-amino-1-(beta-D-ribofuranosyl)-3H-imidazo[4,5-d]pyridazin-7-(6H)-one
-
pH 7.4, 25C
0.00462
4-amino-1-(beta-hydroxyethyl)pyrazolo[3,4d]pyrimidine
-
pH 7.2, 30C
1.8
4-aminopyridine
-
pH 7.5, 25C
1.3
4-aminopyrimidine
-
pH 7.5, 25C
0.0000096
4-fluoro-N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]benzamide
-
30C, pH 7.4
1.4
4-hydroxypyridine
-
pH 7.5, 25C
0.0001556
4-methoxy-N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]benzamide
-
30C, pH 7.3
0.00000079
5'-methylthio-2'-deoxycoformycin
-
-
0.00000043
5'-methylthiocoformycin
-
-
0.0000032
5'-methylthiocoformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.000014
5'-methylthiocoformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.00002
5'-methylthiocoformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.00003
5'-methylthiocoformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.000048
5'-methylthiocoformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.029
5'-methylthiocoformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0265
5-carbamimidoyl-1-(beta-D-ribofuranosyl)-1H-imidazole-4-carbohydrazide
-
pH 7.4, 25C
0.012
7,8-dihydro-1-p-methoxybenzyl-6H-6,7-dimethyl-8-propoxyimidazo[4,5-e][1,2,4]-triazepine
-
pH 7.0, 25C
0.023
7,8-dihydro-1-p-methoxybenzyl-8-(2-methoxyethoxy)-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
pH 7.0, 25C
0.093
7,8-dihydro-1-p-methoxybenzyl-8-(3-methylbenzyloxy)-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
pH 7.0, 25C
0.077
7,8-dihydro-1-p-methoxybenzyl-8-(4-methoxybenzyloxy)-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
pH 7.0, 25C
0.02
7,8-dihydro-8-ethoxy-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
pH 7.0, 25C
0.03
7,8-dihydro-8-isopropoxy-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
pH 7.0, 25C
0.024
7,8-dihydro-8-methoxy-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
pH 7.0, 25C
0.0514
7-amino-1-(beta-D-ribofuranosyl)-1H-imidazo[4,5-d]pyridazin-4-(5H)-one
-
pH 7.4, 25C
0.015
8-butoxy-7,8-dihydro-1-p-methoxybenzyl-6H-6,7-dimethylimidazo[4,5-e][1,2,4]triazepine
-
pH 7.0, 25C
0.126
acetaminophen
-
pH 7.5, 27C
0.214
acetaminophen
-
pH 7.5, 37C
0.0428
Acetylsalicylic acid
-
pH 7.5, 27C
0.0968
Acetylsalicylic acid
-
pH 7.5, 37C
0.17
adenine
-
pH 7.5, 25C
0.00000025
coformycin
-
37C, pH not specified in the publication
0.0000015
D-coformycin
-
mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0000018
D-coformycin
-
mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0000023
D-coformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0000034
D-coformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0000047
D-coformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.000007
D-coformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.0000074
D-coformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.000014
D-coformycin
-
wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication
0.03
diclofenac
-
pH 7.5, 37C
0.0564
diclofenac
-
pH 7.5, 27C
0.00009
diethyl dicarbonate
-
pH 7.1, 23C, in presence of 0.022 mM erythro-9-(2-hydroxy-3-nonyl)-adenine
0.00013
diethyl dicarbonate
-
pH 7.1, 23C, in presence of 0.03 mM erythro-9-(2-hydroxy-3-nonyl)-3-deazaadenine
0.01
diethyl dicarbonate
-
pH 7.1, 23C, in presence of 0.008 mM 1-deazaadenosine
0.0009
erythro-9-(2-hydroxy-3-nonly)adenine
-
mutant D172A, pH 7.5, 30C
-
0.0019
erythro-9-(2-hydroxy-3-nonly)adenine
-
wild-type, pH 7.5, 30C
-
0.00011
erythro-9-(2-hydroxy-3-nonyl)-3-deazaadenine
-
pH 7.1, 23C, after preincubation with diethyl dicarbonate
0.00014
erythro-9-(2-hydroxy-3-nonyl)-3-deazaadenine
-
pH 7.1, 23C
0.00007
erythro-9-(2-hydroxy-3-nonyl)-adenine
-
pH 7.1, 23C, after preincubation with diethyl dicarbonate
0.00008
erythro-9-(2-hydroxy-3-nonyl)-adenine
-
pH 7.1, 23C
0.00000114
erythro-9-(2-hydroxy-3-nonyl)adenine
-
pH 7.2, 30C
0.0000114
erythro-9-(2-hydroxy-3-nonyl)adenine
-
30C, pH 7.15
0.000021
erythro-9-(2-hydroxy-3-nonyl)adenine
-
kidney cortex large isozyme
0.00003
erythro-9-(2-hydroxy-3-nonyl)adenine
-
pleural fluid large isozyme
0.00004
erythro-9-(2-hydroxy-3-nonyl)adenine
-
lung small isozyme
0.000057
erythro-9-(2-hydroxy-3-nonyl)adenine
-
spleen small isozyme
0.00006
erythro-9-(2-hydroxy-3-nonyl)adenine
-
blood serum large isozyme
0.0005
erythro-9-(2-hydroxy-3-nonyl)adenine
-
pH 7.4, 37C
0.000478
N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-2-phenylacetamide
-
30C, pH 7.6
0.000051
N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]-4-(trifluoromethyl)benzamide
-
30C, pH 7.5
0.0001265
N-[4-[(4-oxo-4,5-dihydro-2H-pyrazolo[3,4-d]pyrimidin-2-yl)methyl]phenyl]benzamide
-
30C, pH 7.2
0.018
N6-methyladenosine
-
25C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM
0.25
phenylhydrazine
-
pH 7.5, 25C
1.1
purine
-
pH 7.5, 25C
0.008
purine riboside
-
25C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM
0.016
purine riboside
-
pH 7.5, 25C
0.35
Inosine
-
pH 7.5, 25C
additional information
additional information
-
the adenine moiety of inhibitors does not bind to the histidine residues of the active site His238
-
additional information
additional information
-
inhibition kinetics of the large and the small isozyme
-
additional information
additional information
-
inhibition kinetics of the large isozyme
-
additional information
additional information
-
kinetics and thermodynamic effects of inhibitors, QSAR analysis, overview
-
additional information
additional information
-
inhibition kinetics
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.000027
FR234938
-
pH 7.4, 22C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00000373
-
-
0.004
-
thyroid
0.006
-
kidney
0.007
-
lung
0.008
-
skeletal muscle
0.009
-
liver, cerebellum, spinal cord
0.011
-
cardiac muscle
0.013
-
testis
0.02
-
appendix
0.021
-
adrenal gland
0.023
-
lymph node
0.027
-
pancreas, cerebrum
0.05
-
soluble enzyme
0.06
-
substrate 2,3-O-isopropylidene adenosine, pH 4.0
0.063
-
jejunum
0.072
-
normal B cells
0.076
-
membrane-bound enzyme
0.089
-
stomach
0.12
Q8LPL7
0.033 mM adenosine in 0.2 M phosphate buffer (pH 6.7), 30C
0.127
-
duodenum
0.128
-
spleen
0.136
-
membrane-bound enzyme
0.16
-
soluble enzyme
0.164
-
membrane-bound enzyme
0.191
Staphylococcus aerogenes
-
membrane-bound enzyme
0.2
-
substrate adenosine, pH 4.0
0.281
Micrococcus candidus
-
membrane-bound enzyme
0.366
-
membrane-bound enzyme
0.401
-
membrane-bound enzyme
0.45
-
purified large isozyme from blood serum
0.475
-
T cell line CCRF-HSB-2
0.5
-
substrate 2,3-O-isopropylidene adenosine, pH 5.0; substrate adenosine, pH 5.0
0.501
-
T cell line CCRF-CEM
0.6
-
substrate 2,3-O-isopropylidene adenosine, pH 6.0
0.622
-
membrane-bound enzyme
0.65
-
purified large isozyme from pleural fluid
0.78
-
-
0.8
-
substrate 2,3-O-isopropylidene adenosine, pH 8.0
0.9
-
enzymatic data, conformational properties and docking scores for adenosine analogue 2,3-O-isopropylidenadenosine, modeling studies
0.9
-
substrate 2,3-O-isopropylidene adenosine, pH 7.0
0.965
-
T cell line RPMI-8402
1
-
substrate adenosine
1
-
substrate adenosine, pH 6.0; substrate adenosine, pH 7.0
1.064
-
T cell line MOLT-4
1.1
-
substrate adenosine, pH 8.0
1.2
-
purified isozyme ADA2
1.28
Micrococcus freudenreichii
-
membrane-bound enzyme
1.32
-
enzyme at day 9 of embryogenesis
1.99
-
enzyme at the first day of embryogenesis
2
-
enzymatic data, conformational properties and docking scores for adenosine analogue 2,3-O-methoxymethylideneadenosine, modeling studies
2.16
-
enzyme at day 24 of embryogenesis
2.2
-
protamine sulfate supernatant
2.7
-
El
3.04
-
soluble enzyme
4.4
-
partially purified ADAI
5.3
-
type 1
5.4
-
purified small isozyme from kidney cortex
6.07
-
soluble enzyme
9.07
-
soluble enzyme
10.5
Micrococcus candidus
-
soluble enzyme
17.4
-
Es
19.1
Micrococcus freudenreichii
-
soluble enzyme
28.49
-
-
30.18
-
-
34.2
-
-
52
-
Q Sepharose chromatography
60 - 130
-
purified commercial preparation from spleen
62.7
-
purified ADAII
150.8
-
purified ADAII
166
Q9NZK5
purified isozyme ADA2
252
-
30C
260
-
purine riboside Sepharose chromatography
271
Pseudomonas iodinum
-
-
328
-
-
350
-
purified small isozyme from lung and spleen
372
Micrococcus sodonensis
-
membrane-bound enzyme
400
-
-
834
-
pH 6.8, 25C, enzyme from hemolymph
1200
-
erythrocytes
2029
-
pH 6.8, 37C, enzyme from hemolymph
2180
Micrococcus sodonensis
-
soluble enzyme
2216
-
liver
4400
-
brain
additional information
-
activity in cerebrospinal fluid of healthy persons and tuberculous or non-infectious meningitis patients
additional information
-
enzyme activity in T-lymphocytes of healthy persons and multiple sclerosis patients
additional information
-
enzyme activity in nasal polyps and nasal mucosa
additional information
-
75.7 U/g tissue, ADAII
additional information
-
adenosine deaminase activity is higher in young rat brains when compared to the 60-day-old rats and is higher in hippocampus when compared to the cerebral cortex
additional information
-
no activity with substrate 9H-(1-deoxy-3,4-O-isopropylidene-beta-D-psicofuranosyl)adenine, adenosine deaminase aptly transforms substrates largely modified at the 2- and 3-O-positions, whereas C-modification at the 1-position prevents the adenosine deaminase-catalyzed deamination
additional information
Q9P6I7
326 nkat/mg with substrate adenine, reaction of EC 3.5.4.2, adenine deaminase
additional information
-
tADA and ADA2 levels are higher in patients with Chronic Granulomatous Disease (CGD), Leukocyte Adhesion Deficiency (LAD), hyper IgM (HIM) and Wiskott-Aldrich Syndrome (WAS) than those of corresponding controls. There is a significant elevation of tADA and ADA1 activities in IgA deficiency patients as compared to healthy individuals
additional information
-
relative substrate specificity in nmol NH3/min/mg of protein, adenosine 11.2, 2-deoxyadenosine 23.6, guanosine 1.8, 2-deoxyguanosine 2.0 for the soluble fraction, adenosine 20.9, 2-deoxyadenosine 5.4, guanosine 1.7, 2-deoxyguanosine 1.8 for the membrane-bound adenosine deaminase
additional information
-
patients with fever attack have significantly higher adenosine deaminase levels in plasma compared to attack-free periods and healthy control group
additional information
Q5ZKP6
the adenosine deaminase activity level is highest at day 1 in all the regions of chicken gastrointestinal tract and could be reduced or enhanced by corticosterone and dibutyryl cAMP, respectively, in an age-specific manner
additional information
-
the serum adenosine deaminase activity increases significantly in all infected groups compared to control group (adenosine deaminase activity: 5.99 IU/l control group, 27.62 IU/l infected group)
additional information
-
ADA activity is observed in the parasites at all concentrations tested and its activity is proportional to the concentration of protein, ranging between 0.64 and 2.24 U/l in the lowest and highest concentration of protein, respectively
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
membrane-bound ADA
5.5
-
type intermediate
6 - 6.5
-
-
6 - 7
-
in soluble fraction
6 - 8
-
-
6 - 8
-
-
6 - 8.5
-
-
6.5 - 7.6
-
large isozyme from kidney cortex
6.5 - 7.8
-
large isozyme from blood serum
6.5 - 8.1
-
small isozyme from lung
6.7
Q8LPL7
0.2 M phosphate buffer, 30C
6.7
Q9P6I7
0.2 M phosphate buffer, 33C
6.9 - 8.5
-
-
7 - 10
-
-
7 - 7.4
-
type small and large
7
P53909
0.2 M phosphate buffer, 30-37C
7.2
-
assay at
7.2
-
assay at
7.4
-
assay at
7.4
-
assay at
7.4
-
assay at
7.5
-
assay at
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.5 - 5.5
-
-
3.5 - 9
-
-
4 - 8
-
ciADA activity on adenosine at pH values between 6.0 nd 8.0 remains constant and is lower at pH smaller than 6. The activity is not influenced by the presence of isoproylidene moiety in 2,3-O-isopropylidene adenosine within pH 5-8 range.
5 - 9
-
-
5 - 9
-
20% of maximal activity at pH 5.5, 23% at pH 9.0, pH profile
5 - 9.5
-
ADAII shows 7% of maximal activity at pH 5.5 and 86% at pH 9.0
5.6 - 8.2
Q9NZK5
isozyme ADA2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20
-
assay at
22
-
assay at room temperature
25
-
assay at
27 - 37
-
assay at
30 - 37
P53909
0.2 M phosphate buffer, pH 7.0
30
-
assay at
30
Q8LPL7
0.2 M phosphate buffer, pH 6.7
30
-
assay at
33
Q9P6I7
0.2 M phosphate buffer, pH 6.7
37
Q9NZK5
assay at
37
-
assay at
45
-
the temperature of 37C is chosen for assays in order to lessen the risk of protein denaturation in both cellular fractions
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10 - 80
-
temperature profile
10 - 80
-
activity range of ADAII
20 - 65
-
-
25 - 60
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.9
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
isozyme ADA1
Manually annotated by BRENDA team
-
the activity of isozyme ADA2 is increased in individuals with cancer
Manually annotated by BRENDA team
-
the activity of isozyme ADA2 is increased
Manually annotated by BRENDA team
-
the enzyme activity is highest in tuberculous meningitis patients, followed by non-infectious meningitis patients and healthy persons, the latter showing the lowest activity
Manually annotated by BRENDA team
-
pure enzyme from calf intestine
Manually annotated by BRENDA team
-
enzyme activity during embryogenesis, overview
Manually annotated by BRENDA team
-
type 1, 2 and 2-1
Manually annotated by BRENDA team
-
malaria-infected
Manually annotated by BRENDA team
-
reduced enzyme activity is associated with increased membrane fluidity in transformed versus normal fibroblasts
Manually annotated by BRENDA team
-
T-cell lymphoma cell line
Manually annotated by BRENDA team
-
mucosa, commercial preparation
Manually annotated by BRENDA team
-
peripheral blood
Manually annotated by BRENDA team
-
of peripheral blood
Manually annotated by BRENDA team
-
trophoblast, enzyme activity and its isoenzyme pattern in women with recurrent spontaneous abortions
Manually annotated by BRENDA team
-
from peripheral blood
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
-
differentiating
Manually annotated by BRENDA team
Q9NZK5
ADA2 is secreted by promonocytic cell lines and blood monocytes differentiated into macrophages and dendritic cells
Manually annotated by BRENDA team
additional information
-
distribution of isozymes
Manually annotated by BRENDA team
additional information
-
enzyme activity is increased in chronic fascioliasis
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Micrococcus sodonensis
-
-
Manually annotated by BRENDA team
-
isoyzme ADA2 is secreted by activated monocytes to blood plasma
-
Manually annotated by BRENDA team
Nocardioides sp. J-326TK, Streptomyces sp. J-845S
-
-
-
-
Manually annotated by BRENDA team
Micrococcus sodonensis
-
-
-
Manually annotated by BRENDA team
-
small subunit SADA
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Arthrobacter aurescens (strain TC1)
Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)
Plasmodium vivax (strain Salvador I)
Plasmodium vivax (strain Salvador I)
Plasmodium vivax (strain Salvador I)
Plasmodium vivax (strain Salvador I)
Plasmodium vivax (strain Salvador I)
Salmonella choleraesuis (strain SC-B67)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4100
-
SDS-PAGE
209637
14500
-
gel filtration, type small
209613
26000
-
gel filtration
209628
29000
-
gel filtration
209624
30000 - 35000
-
gel filtration
209607
35480
-
gel filtration
209604
36000 - 44000
-
gel filtration, type a
209615
36000
-
gel filtration, type small
209610
36000
-
gel filtration, type small
209620
36500
-
gel filtration, type large
209620
39000 - 41000
-
gel filtration
209608
39630
P53909
calculated
696861
39950
Q8LPL7
calculated
696861
40000
-
SDS-PAGE
696167
41000
-
SDS-PAGE, calculated value 40754
699651
41000
-
SDS-PAGE
719423
41200
Q9P6I7
calculated
696861
42000
-
a second band of 300000 Da is detected for the complex of adenosine deaminase 1 with dipeptidyl peptidase IV, non-denaturing PAGE
655265
42000
-
ADAII, gel filtration
686577
43000
-
gel filtration
655734
44000
-
SDS-PAGE and nondenaturing PAGE
209622
44700
-
gel filtration
209618
45000
-
SDS-PAGE
209619
45000
-
SDS-PAGE
209627
45000
-
SDS-PAGE
711002
47000
-
Es, gel filtration
209605
48400
-
SDS-PAGE
720969
53700
-
SDS-PAGE
209616
57000
-
gel filtration, type intermediate
209613
66000
-
gel filtration
209623
68000
-
gel filtration
209616
72000
-
SDS-PAGE
209630
80000
-
gel filtration
209630
90000
-
gel filtration
209627
90000
-
HPLC
209629
110000
-
gel filtration
655272
110000
-
isozyme ADA2, gel filtration
667870
114000
-
gel filtration, type intermediate
209610
120000
-
SDS-PAGE
209629
130000
-
gel filtration and sucrose density gradient centrifugation
209606
130000
Micrococcus sodonensis
-
gel filtration
209609
160000
Pseudomonas iodinum
-
gel filtration, absence of ethanol
209611
163000
-
gel filtration, type large
209613
182000
-
gel filtration
720969
230000
-
El, gel filtration
209605
230000
Q58936
gel filtration
727779
240000
Pseudomonas iodinum
-
gel filtration, presence of ethanol
209611
298000
-
gel filtration, type large
209610
298000
-
gel filtration, type b
209615
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 45000, SDS-PAGE
?
-
x * 29000, ADAII, SDS-PAGE
dimer
-
-
dimer
-
2 * 61000, SDS-PAGE
dimer
-
2 * 67000, SDS-PAGE
monomer
-
1 * 43000, SDS-PAGE
monomer
-
44000, SDS-PAGE
monomer
-
45000, SDS-PAGE
monomer
-
72000, SDS-PAGE
monomer
-
26000, gel filtration
monomer
-
1 * 40000 SDS-PAGE
monomer
-
1 * 40000
monomer
-
1 * 42000, enzyme exists as monomer, not in complex with dipeptidyl peptidase IV, SDS-PAGE
monomer
-
1 * 42000, in human liver the adenosine deaminase 1 mainly exists as a complex with dipeptidyl peptidase IV. The molar ratio of adenosine deaminase 1 to dipeptidyl peptidase IV appears to be 2:1, SDS-PAGE
monomer
-
1 x 42000, ADAII, SDS-PAGE
monomer
Nocardioides sp. J-326TK
-
72000, SDS-PAGE
-
polymer
-
-
tetramer
-
SDS-PAGE, 4 * 48400
tetramer
Q58936
4 * 57500
monomer
Klebsiella sp. LF 1202
-
26000, gel filtration
-
additional information
-
structure-activity relationships
additional information
-
the enzyme exists as large and small isozymes, SADA and LADA, the latter is formed by the catalytic subunit monomer, the first by SADA complexed with the ADA complexing protein, i.e. the dipeptidyl peptidase IV
additional information
-
the enzyme has (beta/alpha)8-barrel structure with bound zinc
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
following treatment with N-glycosidase, the molecular weight changes from 61000 Da to 55000 Da
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
commercial enzyme preparation at 20 mg/ml, native enzyme or complexed with inhibitor purine riboside, sitting drop vapour diffusion method, micro-stirring technique over 2 weeks, from 2.2 M ammonium sulfate, 2% 2-methyl-2,4-pentanediol, 0.1 M MES, pH 6.0, 20C, with reservoir solution containing 20% 2-propanol, 20% PEG 4000, 0.1 M citrate, pH 5.6, X-ray diffraction structure determination and analysis at 1.8 A resolution
-
crystal structure of adenosine deaminase ligated with (+)-erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA), vapor diffusion, sitting drop, 2 M ammonium sulfate, 2% 2-methyl-2, 4-pentanediol, 0.1 M MES buffer, pH 6.0, temperature 293K, , X-ray diffraction structure determination and analysis at 2.52 A resolution, space group P 43 21 2, EHNA induces conformational change of adenosine deaminase to the open form in the crystalline state, structural comparison between the EHNA-ADA complex and the 1-eaza-adenosine-ADA complex
-
crystals of the complex of adenosine deaminase with 6-hydroxy-1,6-dihydropurine riboside are grown against reservoirs containing 2.0-2.2 M ammonium sulfate, 2% v/v polyethylene glycol 400 in 0.1 M HEPES buffer, pH 7.5, solved at 2.5 A resolution
-
molecular modeling enzyme-inhibitor, docking simulations enzyme-inhibitor, drug design
-
purified intestinal enzyme complexed with 1-[(R)-1-hydroxy-4-(6-(5-phenylvalerylamino)indol-1-yl)2-butyl]imidazole-4-carboxamide, 1-[(R)-1-hydroxy-4-(6-(3-phenylpropionylamino)indol-1-yl)2-butyl]imidazole-4-carboxamide, or 1-[(R)-4-(6-(3-benzylureido)indol-1-yl)-1-hydroxy-2-butyl]imidazole-4-carboxamide, protein solution is mixed with precipitant solution containing 100 mM MES, pH 6.0, 2.1 ammonium sulfate, and 2.5% v/v PEG 400, X-ray diffraction structure determination and analysis at 2.2 A resolution
-
holo- and apo-ADA, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 200 mM ammonium sulfate, pH 4.7 for apo-ADA or 25% (w/v) PEG 6000, 20 mM Tris-HCl and pH 8.5 for holo-ADA
-
the binding free energies of a series of adenosine deaminase inhibitors are calculated, and the structure-activity relationship is investigated, the inhibitor recognition mechanism of adenosine deaminase and the effect of methyl substitution in inhibitors are discussed on the basis of the analysis of MD trajectories
-
crystal structure of adenosine deaminase from Plasmodium vivax in complex with inhibitor, 2-deoxycoformycin (pentostatin), 33% PEG 20000, 0.1 M TAPS (pH 9.0), 0.1 M Sodium phosphate (monobasic), 16% acetonitrile, 5 mM adenosine, pH 7.0, vapor diffusion, sitting drop, temperature 298K, space group C 2 2 21, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular modeling, crystal structure of adenosine deaminase from Plasmodium vivax in complex with adenosine, space group C 2 2 21, X-ray diffraction structure determination and analysis at 1.89 A resolution, crystal structure of adenosine deaminase from Plasmodium vivax in complex with guanosine, 27.3% PEG 20000, 0.1 M CHES (pH 9.5), 0.1 M Sodium phosphate monobasic, 5 mM guanosine, vapor diffusion, sitting drop, temperature 293K, space group C 2 2 21, X-ray diffraction structure determination and analysis at 2.19 A resolution, these structures highlight a drastic conformational change in plasmodial adenosine deaminase upon substrate binding, these complexes illuminate the structural basis for the differential substrate specificity and potential drug selectivity between mammalian and parasite enzymes
A5KE01
Plasmodium vivax ADA in a complex with 5'-methylthiocoformycin is crystallized by the sitting drop vapor diffusion method, using 25% (w/v) PEG3350, 100 mM HEPES (pH 7.5), and 0.2 M MgCl2
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.5 - 5.5
-
in sodium acetate buffer
209627
6.5 - 7.5
-
-
209630
7 - 11
-
-
209616
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10 - 80
-
stable up to 40C, loss of 85% of activity at 70C, thermal stability profile, overview
686577
25
-
half-life: 636 min
655734
30
-
30C and below
209627
30
-
30C and below
209630
40
-
ADAII, stable up to
686612
50
-
half-life: 61 min
655734
50
-
26% loss of activity after 2 min, 70% loss after 15 min
668123
50
Q58936
10 min, mutant enzyme Y136R/E150R loses 65% of its activity, mutant enzyme Y136R loses 20% of its activity, mutant enzyme E150R loses% of its activity
727779
60
-
ADA is still active when the reactions are carried out at 60C
697984
60
Q58936
10 min, wild-type enzyme loses 10% of its activity, mutant enzyme Y136R loses 60% of its activity, mutant enzyme E150R loses 75% of its activity
727779
68
-
68C and below
209606
70
-
20 min, complete inactivation
655734
70
-
complete loss of activity
719423
70
Q58936
10 min, mutant enzyme loses 66% of its activity
727779
90
-
loss of 95% activity, ADAII
686612
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
50% activity loss after frequent freezing and thawing
-
conversion of smaller enzyme to intermediate and larger enzyme possible
-
Mg2+ or Mn2+ required during purification procedure, addition of serum albumin protects against loss of activity by freezing
Micrococcus sodonensis
-
30% activity loss after heating for 1 h at 60C and neutral pH
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dithiothreitol
-
-
Ethanol
Pseudomonas iodinum
-
-
Ethylene glycol
-
-
Ethylene glycol
Azotobacter vinelandii 0
-
-
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
activity of purified proteins is stable for 1-2 weeks when stored at 4C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20C with 50% (v/v) glycerol and at -80C with 10-20% (v/v) glycerol or as freeze-dried powder
Q8LPL7
-20C, 20% ethylene glycol, several months
-
-20C, KCl-buffer A, glycerol, 8 months
-
-20C, purified large subunit, 2 months without loss of activity
-
2-4C, citrate buffer, pH 6, 2-mercaptoethanol, 12 months
-
room temperature, phosphate buffer, pH 7.0, N-ethylmaleimide
-
4C, 50 mM Tris-HCl, pH 7.5, 0.1 mM adenosine, 0.01 mg enzyme per ml, stable for 6 months
-
4C, Tris-HCl, pH 8.2, 20% ethylene glycol, 2 mM CaCl2, 50M EDTA, several months
-
-20C, PBS, 50% glycerol
-
-20C, purified large subunit, 2 months without loss of activity
-
0C, phosphate buffer, pH 7.0, 2-mercaptoethanol, 1 month, enzyme EI
-
4C, potassium phosphate buffer, pH 7.4, several weeks
-
4C, Tris-HCl, pH 7-9, 3 weeks
-
activity of purified protein is stable for 1-2 weeks when stored at 4C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20C with 50% (v/v) glycerol and at -80C with 10-20% (v/v) glycerol or as freeze-dried powder
P53909
activity of purified protein is stable for 1-2 weeks when stored at 4C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20C with 50% (v/v) glycerol and at -80C with 10-20% (v/v) glycerol or as freeze-dried powder
Q9P6I7
-18C, 50% glycerol, 6 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration, ion exchange chromatography, adenosine deaminase forms a complex with dipeptidyl peptidase II, during purification, it is noted that dipeptidyl peptidase II (DPPII; EC 3.4.14.2) fractions possesses adenosine deaminase activity at all steps
-
small isozyme from lung and spleen to homogeneity, purification of the soluble large isozyme complexed with dipeptidyl peptidase IV from kidney cortex by anion exchange chromatography, gel filtration, and affinity chromatography steps to about 90% purity
-
native ADAI 12.3fold by acetone precipitation and ion exchange chromatography, native ADAII 176.6fold by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity
-
homogeneity, protamine sulfate treatment, Q Sepharose chromatography, purine riboside-Sepharose chromatography
-
isozyme ADA2 400fold from plasma by ammonium sulfate fractionation, anion exchange chromatography, adsorption chromatography, and gel filtration
-
isozyme ADA2, 138.3fold by immunoglobulin and heparin affinity chromatography in two alternated sequences, and gel filtration
Q9NZK5
purification of the soluble large isozyme complexed with dipeptidyl peptidase IV from blood serum and pleural fluid by anion exchange chromatography, gel filtration, and affinity chromatography steps to about 90% purity
-
recombinant isozyme ADA2 is purified by heparin affinity column chromatography, Ni-chelate column chromatography, and gel filtration
Q9NZK5
native enzymes ADAI, ADAII and ADAIII, separation by acetone precipitation and anion exchange chromatography, 2fold purification of ADA1 and 5fold of ADAIII, further purification 62.9fold to homogeneity of ADAII by gel filtration
-
-
Micrococcus sodonensis
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
-
Ni-NTA column chromatography
-
using immobilized metal-affinity chromatography on a Ni-NTA column followed by size-exclusion chromatography on a HiLoad Superdex 200 column
A5KE01
using ion exchange chromatography and gel filtration
-
-
Pseudomonas iodinum
-
using Ni2+-nitrilotriacetate (Ni2+-NTA) column chromatography
P53909
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli BL21 (DE3)
Q8LPL7
enzyme expression in an enzyme-deficient Escherichia coli strain Sphi3834
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expression in Escherichia coli S Phi 3834
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isozyme ADA2 is expressed in S2 Drosophila cells
Q9NZK5
expression in Escherichia coli
Q58936
expression of His-tagged enzyme in Escherichia coli strain BL21
-
expression in Escherichia coli BL21(DE3)
A5KE01
wild type enzyme is expressed in Escherichia coli strain TOP10, mutant enzymes are expressed in Escherichia coli strain BL21-codon plus (DE3)-RIPL
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overexpression in Escherichia coli BL21 (DE3)
P53909
overexpression in Escherichia coli BL21 (DE3)
Q9P6I7
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
HgCl2 does not alter ADA gene expression
-
interferon gamma inhibits secretion of ADA2 by macrophages
Q9NZK5
ADA activity is reduced in serum and erythrocytes in rats infected with Trypanosoma evansi compared to non-infected rats. ADA activity in lymphocytes is decreased after 4 days, when the parasitaemia is high and increases after 20 days, when the number of circulating parasites is low
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the administration of 50 mg/kg methotrexate induces a significant decrease in the ADA activity in the cerebral cortex, kidney and liver, but not in the hippocampus
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activity of ADA in striatum and in hippocampus do not change in acute hyperammonemia as compared to control levels
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subacute hepatitis leads to 135% increase in hepatic ADA activity, in hyperammonemic rats hepatic ADA activity is 3.2fold higher than in control animals. In the cerebellum of hyperammonemic rats ADA activity is increased by 26%. Activity of ADA in the neocortex from hyperammonemic rats is 46% higher than in control rats
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C294S
Q58936
the wild-type and the C294S mutant strains are stable after heating for 10 min at 60C
E150R
Q58936
less thermostable than wild-type enzyme, stable for only 10 min at 50C
Y136R
Q58936
less thermostable than wild-type enzyme, stable for only 10 min at 50C
D172
-
mutant is 40% less active than the wild-type. Mutant is more thightly bound to erythro-9-(2-hydroxy-3-nonly)adenine (Ki: 0.0009 mM) than wild-type
D172A
-
the mutant shows no activity with 5-methylthioadenosine and is not inhibited by 5'-methylthiocoformycin
D172E
-
the mutant shows no activity with 5-methylthioadenosine and is not inhibited by 5'-methylthiocoformycin
additional information
-
effects of sodium dodecyl sulfate, dodecyltrimethylammonium bromide, sodium chloride, sodium sulfate, methanol and ethanol, on the structure and activity of adenosine deaminase are investigated by UV-Vis, circular dichroism spectrophotometry and molecular dynamics studies. Relative activity, experimental and computational helix content, total accessible surface area and exposed charged surface area are obtained. The relative activity of adenosine deaminase in the absence and the presence of denaturants is compared with structural results. It is shown that an increase in the surface area and a decrease in the amount of helicity are associated with a decrease in the activity of ADA
Y136R/E150R
Q58936
no activity with adenosine
additional information
-
ADA-deficiency in mice inhibits the further differentiation of double positive CD4+CD8+ thymocytes in fetal thymic organ culture, the later stages of thymocyte development are sensitive to a lack of ADA, the block is mediated by a mitochondria-dependent mechanism. ADA-deficient cultures are partially rescued by the pan-caspase inhibitor carbobenzoxy-Val-Ala-Asp-fluoromethyl ketone or by the use of Apaf-1-deficient mice lacking apoptotic protease-activating factor-1, overview
additional information
Mus musculus C57BL/6
-
ADA-deficiency in mice inhibits the further differentiation of double positive CD4+CD8+ thymocytes in fetal thymic organ culture, the later stages of thymocyte development are sensitive to a lack of ADA, the block is mediated by a mitochondria-dependent mechanism. ADA-deficient cultures are partially rescued by the pan-caspase inhibitor carbobenzoxy-Val-Ala-Asp-fluoromethyl ketone or by the use of Apaf-1-deficient mice lacking apoptotic protease-activating factor-1, overview
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug development
-
because these enzyme plays an important role in nucleotide metabolism, they are relevant targets in anticancer and antibacterial therapies
diagnostics
-
increased serum adenosine deaminase activity in tropical theileriosis may reflect the involvement of the cellular immune responses
medicine
-
ADA is an adjuvant for a dendritic cell-based vaccine against HIV. ADA potentiates human T-lymphocyte proliferation 4.5fold in co-cultures of HIV-inactivated-pulsed dendritic cells and autologous T cells of HIV-1-infected individuals. ADA enhances cytokine secretion in co-cultures of HIV-pulsed dendritic cells with T cells
diagnostics
-
adenosine deaminase activity in pleural effusions due to Legionnaires disease may be as high as that in pleural effusions due to tuberculosis. In countries where the prevalence of tuberculosis is high and pleural fluid adenosine deaminase activities are frequently measured, Legionnaires disease should be included in the differential diagnosis of an exudative pleural effusion with a high ADA activity
diagnostics
-
adenosine deaminase analysis is a sensitive marker of tuberculous pleuritis even in HIV patients with very low CD4 counts in a high tuberculosis endemic region. The adenosine deaminase assay is inexpensive, rapid, and simple to perform and is of great value for the immediate diagnosis of tuberculous pleuritis while waiting for culture result and this has a positive impact on patient outcome
diagnostics
-
adenosine deaminase is a very useful biochemical parameter in diagnostic analysis tuberculous pleural effusion. When combined with cytologic examination the results are even better
diagnostics
-
adenosine deaminase may be used as a supportive marker to differentiate familial mediterranean fever attacks from attack-free periods
diagnostics
-
serum tADA and ADA2 may serve as useful indicators for diagnosis of CGD, LADs, IgA deficiency, SCID, HIM and WAS patients as a supplementary laboratory test in combination with clinical, immunological, and other laboratory findings
drug development
-
enzyme inhibitor FR234938 might be effective as an anti-rheumatic and anti-inflammatory drug by modulating the host-defense concentrations of adenosine
medicine
-
therapy for adenosine-deficient patients with combined immunodeficiency
medicine
-
genetic polymorphisms of adenosine receptors A1 and A2A are associated with aspirin-intolerant asthma, suggesting that adenosine might play a crucial role in the development of aspirin-intolerant asthma through interactions with the A1 and A2A receptors. In this study no association between ADA and aspirin-intolerant asthma development is found
medicine
-
the adenosine deaminase-adenosine pathway plays a significant role in hemolytic-anemia-associated pulmonary hypertension, and modulation of this pathway may offer protective/therapeutic effects in hemolysis-associated pulmonary hypertension, in particular, under conditions of severe NO deficiency/NO resistance
medicine
Q9NZK5
ADA2 is a drug candidate to modulate the immune responses during inflammation and cancer. ADA2 and its inhibitors are drug candidates to activate the immune systems of immunocompromised patients and to treat various types of blood cancers
medicine
-
the mean values for total ADA and ADA2 activities in the serum and tumor of benign breast disease and breast cancer are significantly higher than those of healthy controls
drug development
-
adenosine deaminase inhibitors have potential as anti-inflammatory drugs or immunosuppressants
drug development
-
progesterone treatment may prevent epileptic activity by decreasing adenosine deaminase levels
medicine
-
therapy for adenosine-deficient patients with combined immunodeficiency
medicine
Nocardioides sp. J-326TK
-
therapy for adenosine-deficient patients with combined immunodeficiency
-
diagnostics
-
the enzym elevle is a useful biochemical parameter for assessing the functional status of liver of sheep with chronicle fascioliasis
pharmacology
-
Plasmodium falciparum-specific inhibitors of adenosine deaminase have potential for development as antimalarials without inhibition of host enzyme
drug development
A5KE01
Plasmodium parasites lack enzymes critical for de novo purine synthesis and thus rely on purine salvage to supply precursors for nucleic acid synthesis and energy metabolism. Thus, the purine salvage pathway is an attractive drug target