EC Number   |
Protein Variants |
Reference |
|---|
   4.2.99.21 | A375T |
loss of the physiological isochorismate synthase catalytic efficiency by three orders of magnitude, and a 2fold gain in isochorismate-pyruvate lyase catalytic efficiency |
729107 |
| 4.2.99.21 | A37I |
mutation increases the rate constant for the chorismate mutase activity by a factor of 1000, and also increases the isochorismate pyruvate lyase catalytic efficiency, by a factor of 6 |
708873 |
| 4.2.99.21 | A43P |
about 25% decrease in both chorismate mutase and isochorismate pyruvate lyase activity |
707496 |
| 4.2.99.21 | C7A |
mutant has a reduced catalytic free energy of activation of up to 0.17 kcal/mol |
729222 |
| 4.2.99.21 | C7A/K42C |
mutant has a reduced catalytic free energy of activation of up to 4.2 kcal/mol. Treatment with bromoethylamine leads to 64% recovery of activity |
729222 |
| 4.2.99.21 | D310E |
physiological isochorismate synthase catalytic efficiency similar to wild-type, 3fold gain in isochorismate-pyruvate lyase catalytic efficiency |
729107 |
| 4.2.99.21 | D310E/A375T |
more than additive effect of the two individual variants with isochorismate-pyruvate lyase catalytic efficiency three orders of magnitude less than wild-type and undetectable salicylate synthase activity |
-, 729107 |
| 4.2.99.21 | D310E/A375T |
similar activity in the isochorismate synthase and isochorismate-pyruvate lyase assays as the A375T variant |
729107 |
| 4.2.99.21 | E240A |
complete loss of activity |
709514 |
| 4.2.99.21 | E281D |
salicylate synthase catalytic efficiency is 32fold less than that of the wildtype enzyme. Isochorismate-pyruvate lyase catalytic efficiency loss of 5fold relative to the wildtype |
-, 729107 |
