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Literature summary for 4.2.99.21 extracted from

  • Olucha, J.; Meneely, K.M.; Lamb, A.L.
    Modification of residue 42 of the active site loop with a lysine-mimetic side chain rescues isochorismate-pyruvate lyase activity in Pseudomonas aeruginosa PchB (2012), Biochemistry, 51, 7525-7532.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.21

Protein Variants

Protein Variants Comment Organism
C7A mutant has a reduced catalytic free energy of activation of up to 0.17 kcal/mol Pseudomonas aeruginosa
C7A/K42C mutant has a reduced catalytic free energy of activation of up to 4.2 kcal/mol. Treatment with bromoethylamine leads to 64% recovery of activity Pseudomonas aeruginosa
K42A mutation leads to the recovery in catalytic free energy of activation of 2.5–2.7 kcal/mol compared to mutant K42C. Exogenous addition of ethylamine to the K42A variant leads to a neglible recovery of activity, whereas addition of propylamine causes an additional modest loss in catalytic power Pseudomonas aeruginosa
K42C mutant has a reduced catalytic free energy of activation of up to 4.4 kcal/mol. Treatment with bromoethylamine leads to 55% recovery of activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 isochorismate wild-type, pH 8.0, 20°C Pseudomonas aeruginosa
0.0021
-
 isochorismate mutant C7A, pH 8.0, 20°C Pseudomonas aeruginosa
0.0156
-
 isochorismate mutant C7A/K42C, treatment with bromoethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.023
-
 isochorismate mutant K42C, treatment with bromoethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.03
-
 isochorismate mutant K42A, pH 8.0, 20°C Pseudomonas aeruginosa
0.048
-
 isochorismate mutant K42A, presence of propylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.06
-
 isochorismate mutant K42A, presence of ethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.114
-
 isochorismate mutant C7A/K42C, treatment with bromoethanol, pH 8.0, 20°C Pseudomonas aeruginosa
0.123
-
 isochorismate mutant C7A/K42C, pH 8.0, 20°C Pseudomonas aeruginosa
0.125
-
 isochorismate mutant K42C, treatment with bromoethanol, pH 8.0, 20°C Pseudomonas aeruginosa
0.134
-
 isochorismate mutant K42C, pH 8.0, 20°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
isochorismate = salicylate + pyruvate the lysine42 residue is required in a specific conformation to stabilize the transition state Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isochorismate
-
 Pseudomonas aeruginosa salicylate + pyruvate
-
 ?

Synonyms

Synonyms Comment Organism
PchB
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0083
-
 isochorismate mutant K42C, pH 8.0, 20°C Pseudomonas aeruginosa
0.0089
-
 isochorismate mutant C7A/K42C, pH 8.0, 20°C Pseudomonas aeruginosa
0.0128
-
 isochorismate mutant C7A/K42C, treatment with bromoethanol, pH 8.0, 20°C Pseudomonas aeruginosa
0.0184
-
 isochorismate mutant K42C, treatment with bromoethanol, pH 8.0, 20°C Pseudomonas aeruginosa
0.045
-
 isochorismate mutant K42A, pH 8.0, 20°C Pseudomonas aeruginosa
0.083
-
 isochorismate mutant K42A, presence of ethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.0945
-
 isochorismate mutant K42C, treatment with bromoethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.098
-
 isochorismate mutant K42A, presence of propylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.105
-
 isochorismate mutant C7A/K42C, treatment with bromoethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
0.13
-
 isochorismate wild-type, pH 8.0, 20°C Pseudomonas aeruginosa
0.2
-
 isochorismate mutant C7A, pH 8.0, 20°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.062
-
 isochorismate mutant K42C, pH 8.0, 20°C Pseudomonas aeruginosa
0.065
-
 isochorismate mutant C7A/K42C, pH 8.0, 20°C Pseudomonas aeruginosa
0.103
-
 isochorismate mutant C7A/K42C, treatment with bromoethanol, pH 8.0, 20°C Pseudomonas aeruginosa
0.147
-
 isochorismate mutant K42C, treatment with bromoethanol, pH 8.0, 20°C Pseudomonas aeruginosa
1.37
-
 isochorismate mutant K42A, presence of propylamine, pH 8.0, 20°C Pseudomonas aeruginosa
1.5
-
 isochorismate mutant K42A, pH 8.0, 20°C Pseudomonas aeruginosa
2.06
-
 isochorismate mutant K42A, presence of ethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
4.2
-
 isochorismate mutant K42C, treatment with bromoethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
6.7
-
 isochorismate mutant C7A/K42C, treatment with bromoethylamine, pH 8.0, 20°C Pseudomonas aeruginosa
93
-
 isochorismate mutant C7A, pH 8.0, 20°C Pseudomonas aeruginosa
124
-
 isochorismate wild-type, pH 8.0, 20°C Pseudomonas aeruginosa