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Literature summary for 4.2.99.21 extracted from

  • Luo, Q.; Olucha, J.; Lamb, A.L.
    Structure-function analyses of isochorismate-pyruvate lyase from Pseudomonas aeruginosa suggest differing catalytic mechanisms for the two pericyclic reactions of this bifunctional enzyme (2009), Biochemistry, 48, 5239-5245.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystallographic structures for mutant K42A with salicylate and pyruvate bound, to 2.5 A resolution, and for apo-I87T, to 2.15 A resolution. Circular dichroism studies of mutants K42A, K42Q, K42E, and K42H, A43P and I87T Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
A43P about 25% decrease in both chorismate mutase and isochorismate pyruvate lyase activity Pseudomonas aeruginosa
I87T structure demonstrates considerable mobility, decrease in both chorismate mutase and isochorismate pyruvate lyase activity Pseudomonas aeruginosa
K42A residue presumably involved in electrostatic transition state stabilization. Active site architecture is maintained in mutant K42A Pseudomonas aeruginosa
K42E almost complete loss of activity Pseudomonas aeruginosa
K42H strong decrease in activity Pseudomonas aeruginosa
K42Q almost complete loss of activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
isochorismate mutant I87T, pH 7.5, 25°C Pseudomonas aeruginosa
0.0043
-
isochorismate wild-type, pH 7.5, 25°C Pseudomonas aeruginosa
0.0053
-
isochorismate mutant A43P, pH 7.5, 25°C Pseudomonas aeruginosa
0.051
-
isochorismate mutant K42A, pH 7.5, 25°C Pseudomonas aeruginosa
0.057
-
isochorismate mutant K42H, pH 7.5, 25°C Pseudomonas aeruginosa
0.066
-
isochorismate mutant K42E, pH 7.5, 25°C Pseudomonas aeruginosa
0.066
-
isochorismate mutant K42H, pH 5.0, 25°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isochorismate
-
Pseudomonas aeruginosa salicylate + pyruvate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0142
-
isochorismate mutant I87T, pH 7.5, 25°C Pseudomonas aeruginosa
0.0245
-
isochorismate mutant K42A, pH 7.5, 25°C Pseudomonas aeruginosa
0.037
-
isochorismate mutant K42H, pH 7.5, 25°C Pseudomonas aeruginosa
0.0468
-
isochorismate mutant K42E, pH 7.5, 25°C Pseudomonas aeruginosa
0.118
-
isochorismate mutant K42H, pH 5.0, 25°C Pseudomonas aeruginosa
0.177
-
isochorismate wild-type, pH 7.5, 25°C Pseudomonas aeruginosa
0.188
-
isochorismate mutant A43P, pH 7.5, 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.48
-
isochorismate mutant K42A, pH 7.5, 25°C Pseudomonas aeruginosa
0.65
-
isochorismate mutant K42H, pH 7.5, 25°C Pseudomonas aeruginosa
0.71
-
isochorismate mutant K42E, pH 7.5, 25°C Pseudomonas aeruginosa
13
-
isochorismate mutant I87T, pH 7.5, 25°C Pseudomonas aeruginosa
17.9
-
isochorismate mutant K42H, pH 5.0, 25°C Pseudomonas aeruginosa
35.5
-
isochorismate mutant A43P, pH 7.5, 25°C Pseudomonas aeruginosa
41
-
isochorismate wild-type, pH 7.5, 25°C Pseudomonas aeruginosa