Crystallization (Comment) | Organism |
---|---|
X-ray crystallographic structures for mutant K42A with salicylate and pyruvate bound, to 2.5 A resolution, and for apo-I87T, to 2.15 A resolution. Circular dichroism studies of mutants K42A, K42Q, K42E, and K42H, A43P and I87T | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
A43P | about 25% decrease in both chorismate mutase and isochorismate pyruvate lyase activity | Pseudomonas aeruginosa |
I87T | structure demonstrates considerable mobility, decrease in both chorismate mutase and isochorismate pyruvate lyase activity | Pseudomonas aeruginosa |
K42A | residue presumably involved in electrostatic transition state stabilization. Active site architecture is maintained in mutant K42A | Pseudomonas aeruginosa |
K42E | almost complete loss of activity | Pseudomonas aeruginosa |
K42H | strong decrease in activity | Pseudomonas aeruginosa |
K42Q | almost complete loss of activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
isochorismate | mutant I87T, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.0043 | - |
isochorismate | wild-type, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.0053 | - |
isochorismate | mutant A43P, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.051 | - |
isochorismate | mutant K42A, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.057 | - |
isochorismate | mutant K42H, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.066 | - |
isochorismate | mutant K42E, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.066 | - |
isochorismate | mutant K42H, pH 5.0, 25°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0142 | - |
isochorismate | mutant I87T, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.0245 | - |
isochorismate | mutant K42A, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.037 | - |
isochorismate | mutant K42H, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.0468 | - |
isochorismate | mutant K42E, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.118 | - |
isochorismate | mutant K42H, pH 5.0, 25°C | Pseudomonas aeruginosa | |
0.177 | - |
isochorismate | wild-type, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.188 | - |
isochorismate | mutant A43P, pH 7.5, 25°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
isochorismate | mutant K42A, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.65 | - |
isochorismate | mutant K42H, pH 7.5, 25°C | Pseudomonas aeruginosa | |
0.71 | - |
isochorismate | mutant K42E, pH 7.5, 25°C | Pseudomonas aeruginosa | |
13 | - |
isochorismate | mutant I87T, pH 7.5, 25°C | Pseudomonas aeruginosa | |
17.9 | - |
isochorismate | mutant K42H, pH 5.0, 25°C | Pseudomonas aeruginosa | |
35.5 | - |
isochorismate | mutant A43P, pH 7.5, 25°C | Pseudomonas aeruginosa | |
41 | - |
isochorismate | wild-type, pH 7.5, 25°C | Pseudomonas aeruginosa |