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Literature summary for 4.2.99.21 extracted from
- Redesign of MST enzymes to target lyase activity instead promotes mutase and dehydratase activities (2013), Arch. Biochem. Biophys., 539, 70-80.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
- |
Yersinia enterocolitica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A375T | loss of the physiological isochorismate synthase catalytic efficiency by three orders of magnitude, and a 2fold gain in isochorismate-pyruvate lyase catalytic efficiency | Pseudomonas aeruginosa |
| D310E | physiological isochorismate synthase catalytic efficiency similar to wild-type, 3fold gain in isochorismate-pyruvate lyase catalytic efficiency | Pseudomonas aeruginosa |
| D310E/A375T | more than additive effect of the two individual variants with isochorismate-pyruvate lyase catalytic efficiency three orders of magnitude less than wild-type and undetectable salicylate synthase activity | Yersinia enterocolitica |
| D310E/A375T | similar activity in the isochorismate synthase and isochorismate-pyruvate lyase assays as the A375T variant | Pseudomonas aeruginosa |
| E281D | salicylate synthase catalytic efficiency is 32fold less than that of the wildtype enzyme. Isochorismate-pyruvate lyase catalytic efficiency loss of 5fold relative to the wildtype | Yersinia enterocolitica |
| T348A | reduction in salicylate synthase catalytic efficiency by five orders of magnitude. Isochorismate-pyruvate lyase catalytic efficiency is reduced by 17fold | Yersinia enterocolitica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00058 | - |
isochorismate | wild-type, 25°C, pH not specified in the publication | Yersinia enterocolitica |  |
| 0.00059 | - |
isochorismate | mutant T348A, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 0.0017 | - |
isochorismate | mutant E281D, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 0.011 | - |
isochorismate | mutant D310E/A375T, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.012 | - |
isochorismate | mutant A375T, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0137 | - |
isochorismate | mutant D310E, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.042 | - |
isochorismate | wild-type, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.047 | - |
isochorismate | mutant E281D/T348A, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Yersinia enterocolitica | Q9X9I8 | - |
- |
| Yersinia enterocolitica ATCC 33114 | Q9X9I8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? | |
| isochorismate | - |
Yersinia enterocolitica | salicylate + pyruvate | - |
? | |
| isochorismate | - |
Yersinia enterocolitica ATCC 33114 | salicylate + pyruvate | - |
? | |
| additional information | isochorismate synthase PhA additionally shows isochorismate lyase activity. Site-specific mutation of active site residues promotes lyase activity | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | salicylate synthase Irp9 additionally shows isochorismate lyase activity | Yersinia enterocolitica | ? | - |
? | |
| additional information | salicylate synthase Irp9 additionally shows isochorismate lyase activity | Yersinia enterocolitica ATCC 33114 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Irp9 | - |
Yersinia enterocolitica |
| PchA | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00038 | - |
isochorismate | mutant D310E/A375T, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0004 | - |
isochorismate | mutant A375T, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.00069 | - |
isochorismate | mutant D310E, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0007 | - |
isochorismate | wild-type, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0055 | - |
isochorismate | mutant T348A, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 0.0343 | - |
isochorismate | mutant E281D/T348A, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 0.05 | - |
isochorismate | mutant E281D, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 0.092 | - |
isochorismate | wild-type, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
isochorismate | wild-type, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0366 | - |
isochorismate | mutant A375T, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.037 | - |
isochorismate | mutant D310E/A375T, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.0504 | - |
isochorismate | mutant D310E, 25°C, pH not specified in the publication | Pseudomonas aeruginosa | |
| 0.74 | - |
isochorismate | mutant E281D/T348A, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 9.3 | - |
isochorismate | mutant T348A, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 31 | - |
isochorismate | mutant E281D, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
| 159 | - |
isochorismate | wild-type, 25°C, pH not specified in the publication | Yersinia enterocolitica | |
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