EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.1.1.20 | 0.021 |
- |
methyl gallate |
mutant enzyme K343A, in 25 mM Tris-HCl (pH 8.0), at 37°C |
730911 |
3.1.1.20 | 0.064 |
- |
Tannic acid |
recombinant tannase, at 30°C, pH 6.0 |
710692 |
3.1.1.20 | 0.078 |
- |
Tannic acid |
endogenous tannase, at 30°C, pH 6.0 |
710692 |
3.1.1.20 | 0.112 |
- |
methyl gallate |
recombinant tannase, at 30°C, pH 6.0 |
710692 |
3.1.1.20 | 0.127 |
- |
methyl gallate |
endogenous tannase, at 30°C, pH 6.0 |
710692 |
3.1.1.20 | 0.42 |
- |
ethyl protocatechuate |
recombinant enzyme, pH 6.0, 30°C |
751144 |
3.1.1.20 | 0.52 |
- |
methyl gallate |
mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C |
730911 |
3.1.1.20 | 2.7 |
- |
methyl gallate |
mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C |
730911 |
3.1.1.20 | 3.45 |
- |
methyl gallate |
at 20°C, in 50 mM citrate buffer at pH 5.0 |
713803 |
3.1.1.20 | 6.04 |
- |
epigallocatechin-3-O-(3-O-methyl) gallate |
in 50 mM Tris-HCl (pH 8.0), at 37°C |
729503 |