Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.20 extracted from

  • Matoba, Y.; Tanaka, N.; Noda, M.; Higashikawa, F.; Kumagai, T.; Sugiyama, M.
    Crystallographic and mutational analyses of tannase from Lactobacillus plantarum (2013), Proteins, 81, 2052-2058.
    View publication on PubMed
Search for more literature for 3.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) and B834(DE3) cells Lactiplantibacillus plantarum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M sodium acetate buffer (pH 5.0) containing 15-20% (w/v) polyethylene glycol 8000 and 0.2 M ammonium sulfate Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
D421A the mutant has 44fold lower activity than the wild type enzyme Lactiplantibacillus plantarum
E357A the mutant has 230fold lower activity than the wild type enzyme Lactiplantibacillus plantarum
K343A the mutant has very little activity compared to the wild type enzyme Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
 methyl gallate wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.95
-
 methyl gallate mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
1
-
 methyl gallate mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Lactiplantibacillus plantarum
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Lactiplantibacillus plantarum SN35N
-
 gallate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum B3Y018
-
-
Lactiplantibacillus plantarum SN35N B3Y018
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + methanol
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus plantarum SN35N gallate + methanol
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum SN35N gallate + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
tannin acylhydrolase
-
 Lactiplantibacillus plantarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
 methyl gallate mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
2.5
-
 methyl gallate mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
74
-
 methyl gallate wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.021
-
 methyl gallate mutant enzyme K343A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.52
-
 methyl gallate mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
2.7
-
 methyl gallate mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
120
-
 methyl gallate wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum