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Literature summary for 3.1.1.20 extracted from

  • Koseki, T.; Ichikawa, K.; Sasaki, K.; Shiono, Y.
    Characterization of a novel Aspergillus oryzae tannase expressed in Pichia pastoris (2018), J. Biosci. Bioeng., 126, 553-558 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene AotanB, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, without the signal sequence, but with secretion signal sequence of Saccharomyces cerevisiae instead of the original signal sequence, recombinant expression in Pichia pastoris strain GS115 from vector pPICZalphaA, the enzyme is secreted into the medium. Recombinant AoTanB is highly expressed by incubation at 18°C compared to 30°C Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
(-)-epigallocatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
0.36
-
(-)-epicatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
0.41
-
(-)-gallocatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
0.86
-
(-)-catechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
0.92
-
ethyl protocatechuate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
1.11
-
methyl gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-catechin gallate + H2O Aspergillus oryzae
-
(-)-catechin + gallic acid
-
?
(-)-catechin gallate + H2O Aspergillus oryzae RIB 40
-
(-)-catechin + gallic acid
-
?
(-)-catechin gallate + H2O Aspergillus oryzae ATCC 42149
-
(-)-catechin + gallic acid
-
?
(-)-epicatechin gallate + H2O Aspergillus oryzae
-
(-)-epicatechin + gallic acid
-
?
(-)-epicatechin gallate + H2O Aspergillus oryzae RIB 40
-
(-)-epicatechin + gallic acid
-
?
(-)-epicatechin gallate + H2O Aspergillus oryzae ATCC 42149
-
(-)-epicatechin + gallic acid
-
?
(-)-epigallocatechin gallate + H2O Aspergillus oryzae
-
(-)-epigallocatechin + gallic acid
-
?
(-)-epigallocatechin gallate + H2O Aspergillus oryzae RIB 40
-
(-)-epigallocatechin + gallic acid
-
?
(-)-epigallocatechin gallate + H2O Aspergillus oryzae ATCC 42149
-
(-)-epigallocatechin + gallic acid
-
?
(-)-gallocatechin gallate + H2O Aspergillus oryzae
-
(-)-gallocatechin + gallic acid
-
?
additional information Aspergillus oryzae the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae ?
-
?
additional information Aspergillus oryzae RIB 40 the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae ?
-
?
additional information Aspergillus oryzae ATCC 42149 the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q2UII1 tanB
-
Aspergillus oryzae ATCC 42149 Q2UII1 tanB
-
Aspergillus oryzae RIB 40 Q2UII1 tanB
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant enzyme expressed in Pichia pastoris, N-deglycosylation Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
recombinant secreted enzyme from Pichia pastoris strain GS115 by anion exchange chromatography and gel filtration Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-catechin gallate + H2O
-
Aspergillus oryzae (-)-catechin + gallic acid
-
?
(-)-catechin gallate + H2O
-
Aspergillus oryzae RIB 40 (-)-catechin + gallic acid
-
?
(-)-catechin gallate + H2O
-
Aspergillus oryzae ATCC 42149 (-)-catechin + gallic acid
-
?
(-)-epicatechin gallate + H2O
-
Aspergillus oryzae (-)-epicatechin + gallic acid
-
?
(-)-epicatechin gallate + H2O
-
Aspergillus oryzae RIB 40 (-)-epicatechin + gallic acid
-
?
(-)-epicatechin gallate + H2O
-
Aspergillus oryzae ATCC 42149 (-)-epicatechin + gallic acid
-
?
(-)-epigallocatechin gallate + H2O
-
Aspergillus oryzae (-)-epigallocatechin + gallic acid
-
?
(-)-epigallocatechin gallate + H2O
-
Aspergillus oryzae RIB 40 (-)-epigallocatechin + gallic acid
-
?
(-)-epigallocatechin gallate + H2O
-
Aspergillus oryzae ATCC 42149 (-)-epigallocatechin + gallic acid
-
?
(-)-gallocatechin gallate + H2O
-
Aspergillus oryzae (-)-gallocatechin + gallic acid
-
?
ethyl protocatechuate + H2O very low activity Aspergillus oryzae ethanol + protocatechuate
-
?
methyl gallate + H2O
-
Aspergillus oryzae methanol + gallic acid
-
?
additional information the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae Aspergillus oryzae ?
-
?
additional information recombinant enzyme AoTanB is able to release gallic acid from natural substrates, such as (-)-catechin gallate, (-)-epicatechin gallate, (-)-gallochatechin gallate, and (-)-epigallocatechin gallate. The enzyme also hydrolyzes ethyl protocatechuate. No activity is detected toward ethyl 4-hydroxybenzoate. The enzyme activity is determined via monitoring of the reaction between gallate and rhodanine Aspergillus oryzae ?
-
?
additional information the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae Aspergillus oryzae RIB 40 ?
-
?
additional information recombinant enzyme AoTanB is able to release gallic acid from natural substrates, such as (-)-catechin gallate, (-)-epicatechin gallate, (-)-gallochatechin gallate, and (-)-epigallocatechin gallate. The enzyme also hydrolyzes ethyl protocatechuate. No activity is detected toward ethyl 4-hydroxybenzoate. The enzyme activity is determined via monitoring of the reaction between gallate and rhodanine Aspergillus oryzae RIB 40 ?
-
?
additional information the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae Aspergillus oryzae ATCC 42149 ?
-
?
additional information recombinant enzyme AoTanB is able to release gallic acid from natural substrates, such as (-)-catechin gallate, (-)-epicatechin gallate, (-)-gallochatechin gallate, and (-)-epigallocatechin gallate. The enzyme also hydrolyzes ethyl protocatechuate. No activity is detected toward ethyl 4-hydroxybenzoate. The enzyme activity is determined via monitoring of the reaction between gallate and rhodanine Aspergillus oryzae ATCC 42149 ?
-
?

Subunits

Subunits Comment Organism
? x * 90000-120000, glycosylated extracellular recombinant enzyme, SDS-PAGE, x * 65000, deglycosylated recombinant extracellular enzyme, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
AotanB
-
Aspergillus oryzae
TanB
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35 recombinant enzyme Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45 activity range, profile overview Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified extracellular recombinant enzyme, pH 6.0, 60 min, stable up to Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
ethyl protocatechuate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
7
-
(-)-epigallocatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
8.5
-
(-)-gallocatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
9.3
-
(-)-epicatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
9.5
-
(-)-catechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
27.7
-
methyl gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
recombinant enzyme Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
4 8 activity range, profile overview Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.42
-
ethyl protocatechuate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
11.05
-
(-)-catechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
20.73
-
(-)-gallocatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
24.95
-
methyl gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
25.83
-
(-)-epicatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae
29.17
-
(-)-epigallocatechin gallate recombinant enzyme, pH 6.0, 30°C Aspergillus oryzae