Cloned (Comment) | Organism |
---|---|
gene AotanB, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, without the signal sequence, but with secretion signal sequence of Saccharomyces cerevisiae instead of the original signal sequence, recombinant expression in Pichia pastoris strain GS115 from vector pPICZalphaA, the enzyme is secreted into the medium. Recombinant AoTanB is highly expressed by incubation at 18°C compared to 30°C | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
(-)-epigallocatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
0.36 | - |
(-)-epicatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
0.41 | - |
(-)-gallocatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
0.86 | - |
(-)-catechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
0.92 | - |
ethyl protocatechuate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
1.11 | - |
methyl gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-catechin gallate + H2O | Aspergillus oryzae | - |
(-)-catechin + gallic acid | - |
? | |
(-)-catechin gallate + H2O | Aspergillus oryzae RIB 40 | - |
(-)-catechin + gallic acid | - |
? | |
(-)-catechin gallate + H2O | Aspergillus oryzae ATCC 42149 | - |
(-)-catechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | Aspergillus oryzae | - |
(-)-epicatechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | Aspergillus oryzae RIB 40 | - |
(-)-epicatechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | Aspergillus oryzae ATCC 42149 | - |
(-)-epicatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | Aspergillus oryzae | - |
(-)-epigallocatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | Aspergillus oryzae RIB 40 | - |
(-)-epigallocatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | Aspergillus oryzae ATCC 42149 | - |
(-)-epigallocatechin + gallic acid | - |
? | |
(-)-gallocatechin gallate + H2O | Aspergillus oryzae | - |
(-)-gallocatechin + gallic acid | - |
? | |
additional information | Aspergillus oryzae | the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae | ? | - |
? | |
additional information | Aspergillus oryzae RIB 40 | the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae | ? | - |
? | |
additional information | Aspergillus oryzae ATCC 42149 | the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q2UII1 | tanB | - |
Aspergillus oryzae ATCC 42149 | Q2UII1 | tanB | - |
Aspergillus oryzae RIB 40 | Q2UII1 | tanB | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant enzyme expressed in Pichia pastoris, N-deglycosylation | Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
recombinant secreted enzyme from Pichia pastoris strain GS115 by anion exchange chromatography and gel filtration | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-catechin gallate + H2O | - |
Aspergillus oryzae | (-)-catechin + gallic acid | - |
? | |
(-)-catechin gallate + H2O | - |
Aspergillus oryzae RIB 40 | (-)-catechin + gallic acid | - |
? | |
(-)-catechin gallate + H2O | - |
Aspergillus oryzae ATCC 42149 | (-)-catechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | - |
Aspergillus oryzae | (-)-epicatechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | - |
Aspergillus oryzae RIB 40 | (-)-epicatechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | - |
Aspergillus oryzae ATCC 42149 | (-)-epicatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | - |
Aspergillus oryzae | (-)-epigallocatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | - |
Aspergillus oryzae RIB 40 | (-)-epigallocatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | - |
Aspergillus oryzae ATCC 42149 | (-)-epigallocatechin + gallic acid | - |
? | |
(-)-gallocatechin gallate + H2O | - |
Aspergillus oryzae | (-)-gallocatechin + gallic acid | - |
? | |
ethyl protocatechuate + H2O | very low activity | Aspergillus oryzae | ethanol + protocatechuate | - |
? | |
methyl gallate + H2O | - |
Aspergillus oryzae | methanol + gallic acid | - |
? | |
additional information | the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae | Aspergillus oryzae | ? | - |
? | |
additional information | recombinant enzyme AoTanB is able to release gallic acid from natural substrates, such as (-)-catechin gallate, (-)-epicatechin gallate, (-)-gallochatechin gallate, and (-)-epigallocatechin gallate. The enzyme also hydrolyzes ethyl protocatechuate. No activity is detected toward ethyl 4-hydroxybenzoate. The enzyme activity is determined via monitoring of the reaction between gallate and rhodanine | Aspergillus oryzae | ? | - |
? | |
additional information | the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae | Aspergillus oryzae RIB 40 | ? | - |
? | |
additional information | recombinant enzyme AoTanB is able to release gallic acid from natural substrates, such as (-)-catechin gallate, (-)-epicatechin gallate, (-)-gallochatechin gallate, and (-)-epigallocatechin gallate. The enzyme also hydrolyzes ethyl protocatechuate. No activity is detected toward ethyl 4-hydroxybenzoate. The enzyme activity is determined via monitoring of the reaction between gallate and rhodanine | Aspergillus oryzae RIB 40 | ? | - |
? | |
additional information | the activity of recombinant AoTanB is lower toward natural substrates compared to that of AoTanA from Aspergillus oryzae | Aspergillus oryzae ATCC 42149 | ? | - |
? | |
additional information | recombinant enzyme AoTanB is able to release gallic acid from natural substrates, such as (-)-catechin gallate, (-)-epicatechin gallate, (-)-gallochatechin gallate, and (-)-epigallocatechin gallate. The enzyme also hydrolyzes ethyl protocatechuate. No activity is detected toward ethyl 4-hydroxybenzoate. The enzyme activity is determined via monitoring of the reaction between gallate and rhodanine | Aspergillus oryzae ATCC 42149 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000-120000, glycosylated extracellular recombinant enzyme, SDS-PAGE, x * 65000, deglycosylated recombinant extracellular enzyme, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
AotanB | - |
Aspergillus oryzae |
TanB | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | recombinant enzyme | Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 45 | activity range, profile overview | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified extracellular recombinant enzyme, pH 6.0, 60 min, stable up to | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
ethyl protocatechuate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
7 | - |
(-)-epigallocatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
8.5 | - |
(-)-gallocatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
9.3 | - |
(-)-epicatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
9.5 | - |
(-)-catechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
27.7 | - |
methyl gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | activity range, profile overview | Aspergillus oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
ethyl protocatechuate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
11.05 | - |
(-)-catechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
20.73 | - |
(-)-gallocatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
24.95 | - |
methyl gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
25.83 | - |
(-)-epicatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae | |
29.17 | - |
(-)-epigallocatechin gallate | recombinant enzyme, pH 6.0, 30°C | Aspergillus oryzae |