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Literature summary for 3.1.1.20 extracted from

  • Ueda, S.; Nomoto, R.; Yoshida, K.; Osawa, R.
    Comparison of three tannases cloned from closely related Lactobacillus species: L. plantarum, L. paraplantarum, and L. pentosus (2014), BMC Microbiol., 14, 87.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.20

Activating Compound

Activating Compound Comment Organism Structure
EDTA 103.7% activity at 1 mM Lactiplantibacillus pentosus
Urea 119.5% activity at 1 mM Lactiplantibacillus pentosus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in in Bacillus subtilis strain RIK 1285 Lactiplantibacillus pentosus
expressed in in Bacillus subtilis strain RIK 1285 Lactiplantibacillus plantarum
expressed in in Bacillus subtilis strain RIK 1285 Lactiplantibacillus paraplantarum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 88.7% residual activity at 1 mM Lactiplantibacillus paraplantarum
Ca2+ 92.3% residual activity at 1 mM Lactiplantibacillus pentosus
Ca2+ 98.3% residual activity at 1 mM Lactiplantibacillus plantarum
EDTA the enzyme is partially inhibited by but not significantly affected (87.8% residual activity at 1 mM) Lactiplantibacillus paraplantarum
EDTA the enzyme is partially inhibited by but not significantly affected (97.6% residual activity at 1 mM) Lactiplantibacillus plantarum
Fe2+ 24.1% residual activity at 1 mM Lactiplantibacillus paraplantarum
Fe2+ 23.4% residual activity at 1 mM Lactiplantibacillus pentosus
Fe2+ 22.5% residual activity at 1 mM Lactiplantibacillus plantarum
Mg2+ 96.7% residual activity at 1 mM Lactiplantibacillus pentosus
Mn2+ 75.6% residual activity at 1 mM Lactiplantibacillus pentosus
Mn2+ 87.6% residual activity at 1 mM Lactiplantibacillus plantarum
phenylmethylsulfonyl fluoride the enzyme is partially inhibited by but not significantly affected (66.2% residual activity at 1 mM) Lactiplantibacillus paraplantarum
phenylmethylsulfonyl fluoride 81.2% residual activity at 1 mM Lactiplantibacillus pentosus
phenylmethylsulfonyl fluoride the enzyme is partially inhibited by but not significantly affected (83.2% residual activity at 1 mM) Lactiplantibacillus plantarum
Urea the enzyme is partially inhibited by but not significantly affected (96.9% residual activity at 1 mM) Lactiplantibacillus paraplantarum
Urea the enzyme is partially inhibited by but not significantly affected (91.4% residual activity at 1 mM) Lactiplantibacillus plantarum
Zn2+ 95.4% residual activity at 1 mM Lactiplantibacillus paraplantarum
Zn2+ 25.2% residual activity at 1 mM Lactiplantibacillus pentosus
Zn2+ 46.1% residual activity at 1 mM Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.03
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.03
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.04
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.04
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
0.05
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.05
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.05
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
0.06
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.06
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
0.06
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
0.06
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
0.07
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.08
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.1
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.37
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.5
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
0.87
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 110.5% activity at 1 mM Lactiplantibacillus paraplantarum
Mg2+ 123.7% activity at 1 mM Lactiplantibacillus plantarum
Mn2+ 111.3% activity at 1 mM Lactiplantibacillus paraplantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Lactiplantibacillus pentosus
50000
-
 x * 50000, SDS-PAGE Lactiplantibacillus plantarum
50000
-
 x * 50000, SDS-PAGE Lactiplantibacillus paraplantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Lactiplantibacillus pentosus
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Lactiplantibacillus plantarum
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Lactiplantibacillus paraplantarum
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Lactiplantibacillus pentosus 21A-3
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Lactiplantibacillus paraplantarum NSO120
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Lactiplantibacillus plantarum ATCC 14917T
-
 gallate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus paraplantarum
-
-
-
Lactiplantibacillus paraplantarum NSO120
-
-
-
Lactiplantibacillus pentosus
-
-
-
Lactiplantibacillus pentosus 21A-3
-
-
-
Lactiplantibacillus plantarum B3Y018
-
-
Lactiplantibacillus plantarum ATCC 14917T B3Y018
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Lactiplantibacillus pentosus
TALON metal affinity resin column chromatography Lactiplantibacillus plantarum
TALON metal affinity resin column chromatography Lactiplantibacillus paraplantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechin gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
catechin gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
catechin gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
catechin gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 ?
-
 ?
catechin gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 ?
-
 ?
catechin gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T ?
-
 ?
epicatechin gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
epicatechin gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
epicatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
epicatechin gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 ?
-
 ?
epicatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 ?
-
 ?
epicatechin gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T ?
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus pentosus gallate + epigallocatechin
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum gallate + epigallocatechin
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum gallate + epigallocatechin
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 gallate + epigallocatechin
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 gallate + epigallocatechin
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T gallate + epigallocatechin
-
 ?
epigallocatechin-3-O-(3-O-methyl) gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
epigallocatechin-3-O-(3-O-methyl) gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
epigallocatechin-3-O-(3-O-methyl) gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
gallocatechin gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
gallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
gallocatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus pentosus gallate + methanol
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + methanol
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus paraplantarum gallate + methanol
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 gallate + methanol
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 gallate + methanol
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T gallate + methanol
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus pentosus gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus paraplantarum gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus pentosus 21A-3 gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus paraplantarum NSO120 gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T gallate + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Lactiplantibacillus pentosus
? x * 50000, SDS-PAGE Lactiplantibacillus plantarum
? x * 50000, SDS-PAGE Lactiplantibacillus paraplantarum

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
 Lactiplantibacillus pentosus
tannin acyl hydrolase
-
 Lactiplantibacillus plantarum
tannin acyl hydrolase
-
 Lactiplantibacillus paraplantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Lactiplantibacillus pentosus
40
-
-
 Lactiplantibacillus plantarum
40
-
-
 Lactiplantibacillus paraplantarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.26
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
0.35
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
0.42
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.44
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
0.89
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
1.12
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
1.12
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
1.48
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
1.49
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
2.41
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
8.1
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
9.2
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
11.08
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
14.29
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
15.95
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
46.02
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
72.73
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Lactiplantibacillus pentosus
8.5
-
-
 Lactiplantibacillus plantarum
8.5
-
-
 Lactiplantibacillus paraplantarum

pH Range

pH Minimum pH Maximum Comment Organism
8 10 the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 Lactiplantibacillus pentosus
8 10 the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 Lactiplantibacillus plantarum
8 10 the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 Lactiplantibacillus paraplantarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.04
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
7.25
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
8.63
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
9.02
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
10.57
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
11.68
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
14.32
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
18.79
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
19.22
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
27.19
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
52.23
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
53.65
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
125
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
145.1
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
154.7
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
181.5
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
195.3
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
260.8
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum