EC Number   |
General Information |
Reference |
|---|
   6.5.1.8 | malfunction |
RNAi knockdown of rtcb-1 lowers endoplasmic reticulum stress response in vivo |
748446 |
| 6.5.1.8 | metabolism |
the enzyme is necessary for xbp-1 mRNA splicing |
748446 |
| 6.5.1.8 | physiological function |
archease and RtcB from Pyrococcus horikoshii function in tandem, with Archease altering the catalytic properties of the RNA ligase. Catalytic concentrations of archease are sufficient to activate RtcB, and Archease accelerates both the RNA 3'-P guanylylation and ligation steps. Archease can alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently. RtcB variants that have inactivating substitutions in the guanine-binding pocket can be rescued by the addition of archease. Substitution of the archease metal-binding residues drastically reduces archease-dependent activation of RtcB |
-, 741076 |
| 6.5.1.8 | physiological function |
enzyme catalyzes the GTP-dependent ligation of RNA with 3'-phosphate and 5'-hydroxyl termini. RtcB can ligate RNA cleaved by RNA endonucleases, which generate 2',3'-cyclic phosphate and then 3'-phosphate termini on one strand, and a 5'-hydroxyl terminus on another strand |
740053 |
| 6.5.1.8 | physiological function |
fungal tRNA ligase Trl1 is an essential enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends inflicted during tRNA splicing and noncanonical mRNA splicing in the fungal unfolded protein response |
748786 |
| 6.5.1.8 | physiological function |
RtcB as the primary unfolded protein response RNA ligase. In RtcB knockout cells, unconventional XBP1 mRNA splicing is defective during ER stress. The RNA ligase activity of RtcB is directly required for the splicing of XBP1 mRNA |
741005 |
| 6.5.1.8 | physiological function |
RtcB from Thermobifida fusca is a single-turnover enzyme that is unable to be converted into a multiple-turnover ligase by archease from other organisms |
741398 |
| 6.5.1.8 | physiological function |
RtcB from Thermus thermophilus displays a strong dependency on Archease for maximal activity and can catalyze multiple turnovers only in the presence of archease. Archease from P. horikoshii can activate Thermus thermophilus RtcB |
741398 |
| 6.5.1.8 | physiological function |
RtcB guanylylation precedes the cyclic phosphodiesterase and 3'-phosphate ligase steps of the RNA splicing pathway |
741074 |
| 6.5.1.8 | physiological function |
RtcB is able in vivo to complement growth of yeast cells that lack the endogenous healing/sealing-type tRNA ligase Trl1. RtcB also protects yeast Trl1 mutant cells against a fungal ribotoxin that incises the anticodon loop of cellular tRNAs. RtcB can replace Trl1 as the catalyst of HAC1 mRNA splicing during the unfolded protein response |
740694 |
