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Literature summary for 6.5.1.8 extracted from

  • Tanaka, N.; Meineke, B.; Shuman, S.
    RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo (2011), J. Biol. Chem., 286, 30253-30257.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Escherichia coli

Protein Variants

Protein Variants Comment Organism
C78A almost complete loss of activity Escherichia coli
C78S complete loss of activity Escherichia coli
D75A almost complete loss of activity Escherichia coli
D75E complete loss of activity Escherichia coli
D75N complete loss of activity Escherichia coli
H168A almost complete loss of activity Escherichia coli
H168N complete loss of activity Escherichia coli
H168Q complete loss of activity Escherichia coli
H185A activity is severely impaired Escherichia coli
H280A active, protein is able to complement a Saccharomyces cerevisiae Trl1 mutant Escherichia coli
H337A almost complete loss of activity Escherichia coli
H337N complete loss of activity Escherichia coli
H337Q complete loss of activity Escherichia coli
K298A activity is severely impaired Escherichia coli
N167A activity is severely impaired Escherichia coli
R189A almost complete loss of activity Escherichia coli
R189A activity is severely impaired Escherichia coli
R341A almost complete loss of activity Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Escherichia coli
additional information Mg2+ cannot substitute for Mn2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P46850
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O substrate mimicks the broken tRNAGlu(UUC) anticodon stem-loop generated by Kluyveromyces lactis gamma-toxin, leaving 2',3'-cyclic phosphate and 5'-OH ends Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
-
Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction, substrate 5'-hydroxy-(ribonucleotide)m can be composed of all ribonucleotides or all deoxynucleotides ?
(ribonucleotide)n-3'-phosphate + 5'-phospho-(ribonucleotide)m + GTP
-
Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction ?

General Information

General Information Comment Organism
physiological function RtcB is able in vivo to complement growth of yeast cells that lack the endogenous healing/sealing-type tRNA ligase Trl1. RtcB also protects yeast Trl1 mutant cells against a fungal ribotoxin that incises the anticodon loop of cellular tRNAs. RtcB can replace Trl1 as the catalyst of HAC1 mRNA splicing during the unfolded protein response Escherichia coli