Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
C98A | mutation in predicted metal-binding site, loss of activity | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | absolutely required | Pyrococcus furiosus | |
additional information | Zn2+ cannot replace Mn2+ | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U0H4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | - |
Pyrococcus furiosus | (ribonucleotide)n+m + GMP + diphosphate | overall reaction | ? | |
(ribonucleotide)n-2',3'-cyclophosphate + H2O | - |
Pyrococcus furiosus | (ribonucleotide)n-3'-phosphate | - |
? | |
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m | - |
Pyrococcus furiosus | (ribonucleotide)n+m + GMP | - |
? | |
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate | - |
Pyrococcus furiosus | (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine | - |
? | |
GTP + [RNA ligase]-L-histidine | - |
Pyrococcus furiosus | 5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + diphosphate | - |
? | |
additional information | three-step mechanism for catalysis of the ligation of 3'-P and 5'-OH RNA termini by RtcB. In the activation step, cleavage of the phosphoanhydride bond between the alpha and beta phosphoryl groups of GTP occurs during transfer of GMP to the 3'-P terminus of an RNA strand to form a guanylylate-activated intermediate. In the cyclization step, the 2'-OH on the terminal ribose attacks the GMP-activated 3'-P to generate a 2',3'-cyclic phosphate and release GMP. In the ligation step, the 5'-OH terminus of a second RNA strand attacks the cyclic phosphate to form a 3',5'-phosphodiester bond | Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rtcB | - |
Pyrococcus furiosus |
tRNA-splicing ligase | - |
Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme catalyzes the GTP-dependent ligation of RNA with 3'-phosphate and 5'-hydroxyl termini. RtcB can ligate RNA cleaved by RNA endonucleases, which generate 2',3'-cyclic phosphate and then 3'-phosphate termini on one strand, and a 5'-hydroxyl terminus on another strand | Pyrococcus furiosus |