Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.8 extracted from

  • Chakravarty, A.K.; Shuman, S.
    The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent (2012), Nucleic Acids Res., 40, 8558-8567.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.8

Protein Variants

Protein Variants Comment Organism
H337A His337 is the site covalent guanylylation of RtcB, mutant is inactive Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ absolutely required, assay at 2 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P46850
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O substrate for ligation is a 20mer RNA strand with 5'-OH and 2',3'-cyclic phosphate ends Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction ?
5'-guanosyl [RNA ligase]-NT-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
-
 Escherichia coli (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine RtcB transfers GMP to the RNA 3'-phosphate end ?
GTP + [RNA ligase]-L-histidine
-
 Escherichia coli 5'-guanosyl [RNA ligase]-NT-phosphono-L-histidine + diphosphate
-
 ?
additional information sealing of a 2',3'-cyclic phosphate end by RtcB requires GTP, is contingent on formation of the RtcB–GMP adduct, and involves a kinetically valid RNA(3')pp(5')G intermediate. RtcB catalyzes the hydrolysis of a 2',3'-cyclic phosphate to a 3'-phosphate at a rate that is at least as fast as the rate of ligation. NTP requirement for ligation is satisfied by GTP and 6-O-methyl guanosine triphosphate and inosine triphosphate. 6-chloropurine ribonucleoside triphosphate, 2-aminopurine ribonucleoside triphosphate and ATP are inactive Escherichia coli ?
-
 ?

General Information

General Information Comment Organism
physiological function RtcB guanylylation precedes the cyclic phosphodiesterase and 3'-phosphate ligase steps of the RNA splicing pathway Escherichia coli