EC Number   |
Metals/Ions |
Reference |
|---|
   3.1.30.1 | Co2+ |
up to 3fold stimulation of DNA hydrolysis rate by 0.5 mM |
134971 |
| 3.1.30.1 | Co2+ |
with 1 mM restoration of 72% of activity of 1 mM EDTA-treated enzyme |
134978 |
| 3.1.30.1 | Fe2+ |
10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions, strong cleavage results from attack by hydroxyl radicals generated from Fe2+ ions bound into the active site of endonuclease I |
656529 |
| 3.1.30.1 | Fe2+ |
2.5fold stimulation of hydrolysis rate of denaturated DNA by 10 mM |
134971 |
| 3.1.30.1 | Fe2+ |
can only partially substitute for Zn2+ |
718072 |
| 3.1.30.1 | Hg2+ |
maximal activity at 0.01-1 mM at pH 3.5-5.0 |
134964 |
| 3.1.30.1 | KCl |
required |
718072 |
| 3.1.30.1 | Mg2+ |
- |
134971, 134999 |
| 3.1.30.1 | Mg2+ |
10 mM, cleavage of single base pair mismatches |
656882 |
| 3.1.30.1 | Mg2+ |
10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions |
656529 |
