Cloned (Comment) | Organism |
---|---|
- |
Escherichia phage T7 |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia phage T7 |
Protein Variants | Comment | Organism |
---|---|---|
D55N | 3fold higher DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
E20A | 3fold higher DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
E20Q | similar DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
E65K | 2fold higher DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
E65Q | similar DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
K67A | 2fold higher DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
K67R | similar DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
P54L | 3fold higher DNA-junction cleavage rate as wild-type | Escherichia phage T7 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, complete inhibition of junction cleavage | Escherichia phage T7 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions | Escherichia phage T7 | |
Fe2+ | 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions, strong cleavage results from attack by hydroxyl radicals generated from Fe2+ ions bound into the active site of endonuclease I | Escherichia phage T7 | |
Mg2+ | 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions | Escherichia phage T7 | |
Mn2+ | 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions | Escherichia phage T7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia phage T7 | resolving of DNA-four way junction | nicked DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage T7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant endonuclease I | Escherichia phage T7 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | resolving of DNA-four way junction | Escherichia phage T7 | nicked DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia phage T7 |