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Literature summary for 3.1.30.1 extracted from

  • Freeman, A.D.J.; Declais, A.C.; Lilley, D.M.J.
    Metal ion binding in the active site of the junction-resolving enzyme T7 endonuclease I in the presence and in the absence of DNA (2003), J. Mol. Biol., 333, 59-73.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia phage T7

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia phage T7

Protein Variants

Protein Variants Comment Organism
D55N 3fold higher DNA-junction cleavage rate as wild-type Escherichia phage T7
E20A 3fold higher DNA-junction cleavage rate as wild-type Escherichia phage T7
E20Q similar DNA-junction cleavage rate as wild-type Escherichia phage T7
E65K 2fold higher DNA-junction cleavage rate as wild-type Escherichia phage T7
E65Q similar DNA-junction cleavage rate as wild-type Escherichia phage T7
K67A 2fold higher DNA-junction cleavage rate as wild-type Escherichia phage T7
K67R similar DNA-junction cleavage rate as wild-type Escherichia phage T7
P54L 3fold higher DNA-junction cleavage rate as wild-type Escherichia phage T7

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, complete inhibition of junction cleavage Escherichia phage T7

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions Escherichia phage T7
Fe2+ 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions, strong cleavage results from attack by hydroxyl radicals generated from Fe2+ ions bound into the active site of endonuclease I Escherichia phage T7
Mg2+ 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions Escherichia phage T7
Mn2+ 10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions Escherichia phage T7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia phage T7 resolving of DNA-four way junction nicked DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia phage T7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant endonuclease I Escherichia phage T7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O resolving of DNA-four way junction Escherichia phage T7 nicked DNA
-
?

Subunits

Subunits Comment Organism
dimer
-
Escherichia phage T7