Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Penicillium melinii | |
EDTA | reversible by Zn2+ | Penicillium melinii | |
iodoacetamide | - |
Penicillium melinii | |
KH2PO4 | - |
Penicillium melinii | |
additional information | urea does not influence the enzyme at any concentrations | Penicillium melinii | |
potassium citrate | complete inhibition at 10 mM | Penicillium melinii | |
SDS | - |
Penicillium melinii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Penicillium melinii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can only partially substitute for Zn2+ | Penicillium melinii | |
Co2+ | can only partially substitute for Zn2+ | Penicillium melinii | |
Fe2+ | can only partially substitute for Zn2+ | Penicillium melinii | |
KCl | required | Penicillium melinii | |
Mn2+ | can only partially substitute for Zn2+ | Penicillium melinii | |
NaCl | required | Penicillium melinii | |
Zn2+ | required | Penicillium melinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Penicillium melinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Penicillium melinii | the enzyme is active on DNA and RNA, the enzyme endonucleolytically degrades ssDNA and RNA by 3'-5' mode to produce 5'-oligonucleotides and 5'-mononucleotides. It preferentially degrades poly(U), substrate specificity, overview | ? | - |
? | |
poly(U) + H2O | Penicillium melinii | preferred substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium melinii | - |
isolated from colonial ascidium collected near Shikotan Island, Sea of Okhotsk, at a depth of 123 m | - |
Purification (Comment) | Organism |
---|---|
extracellular enzyme 165fold by chromatography with modified chitosan | Penicillium melinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41250 | - |
purified enzyme, pH 3.7, 75°C | Penicillium melinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is active on DNA and RNA, the enzyme endonucleolytically degrades ssDNA and RNA by 3'-5' mode to produce 5'-oligonucleotides and 5'-mononucleotides. It preferentially degrades poly(U), substrate specificity, overview | Penicillium melinii | ? | - |
? | |
poly(U) + H2O | preferred substrate | Penicillium melinii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Penicillium melinii |
Synonyms | Comment | Organism |
---|---|---|
S1-type nuclease | - |
Penicillium melinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Penicillium melinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.7 | - |
- |
Penicillium melinii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | activity range | Penicillium melinii |
General Information | Comment | Organism |
---|---|---|
additional information | mode of nuclease action and molecular modeling, overview | Penicillium melinii |
physiological function | tight correlation found between the extracellular endonuclease activity and the rate of thymidine uptake by actively growing cells suggesting that this nuclease is required for fulfilling the nucleotide pool of precursors of DNA biosynthesis during the transformation of hyphae into the aerial mycelium and conidia in stressful environmental conditions | Penicillium melinii |