Literature summary for 3.1.30.1 extracted from

Wiegand, R.C.; Godson, G.N.; Radding, C.M.
Specificity of the S1 nuclease from Aspergillus oryzae (1975), J. Biol. Chem., 250, 8848-8855.

Search for more literature for 3.1.30.1

Application

Application	Comment	Organism
analysis	analysis of heteroduplex and superhelical DNA, quantitation of nucleic acid hybridization	Aspergillus oryzae

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-dAMP	50% inhibition by 0.085 mM dAMP	Aspergillus oryzae
5'-dATP	50% inhibition by 0.001 mM dATP	Aspergillus oryzae
phosphate	50% inhibition by 2 mM sodium phosphate	Aspergillus oryzae
sodium bisphosphate	50% inhibition by 0.02 mM sodium bisphosphate	Aspergillus oryzae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	-	Aspergillus oryzae
Hg2+	maximal activity at 0.01-1 mM at pH 3.5-5.0	Aspergillus oryzae
Zn2+	-	Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA + H2O	Aspergillus oryzae	75000 times more active on single-stranded than on native DNA	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus oryzae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
12.7	-	purified enzyme, heat-denaturated DNA as substrate	Aspergillus oryzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	75000 times more active on single-stranded than on native DNA	Aspergillus oryzae	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?
dsDNA + H2O	activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA	Aspergillus oryzae	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?

Synonyms

Synonyms	Comment	Organism
S1 nuclease	-	Aspergillus oryzae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5	5	-	Aspergillus oryzae
4.5	-	in 10 mM NaCl	Aspergillus oryzae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	-	5'-dATP	-	Aspergillus oryzae
0.085	-	5'-dAMP	-	Aspergillus oryzae

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Release 2023.1 (January 2023)