EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference  |
|---|
   1.14.15.8 | 0.037 |
- |
11-deoxycortisol |
pH 7.4, 30°C, mutant A106T/Q189K/T399S/R409L, mutant D153V/I214F |
693956 |
| 1.14.15.8 | 0.059 |
- |
11-deoxycortisol |
pH 7.4, 30°C, mutant A106T |
693956 |
| 1.14.15.8 | 0.066 |
- |
11-deoxycortisol |
pH 7.4, 30°C, mutant D217V |
693956 |
| 1.14.15.8 | 0.068 |
- |
11-deoxycortisol |
pH 7.4, 30°C, mutant A106T/R409L |
693956 |
| 1.14.15.8 | 0.093 |
- |
11-deoxycortisol |
pH 7.4, 30°C, CYP106A2 wild-type enzyme |
693956 |
| 1.14.15.8 | 0.251 |
- |
progesterone |
pH 7.4, 30°C, for determination of Km-value the formation of the product 15beta-hydroxy-progesterone is determined |
695981 |
| 1.14.15.8 | -999 |
- |
more |
KM-value for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2 |
696462 |
| 1.14.15.8 | 0.00314 |
- |
reduced ferredoxin |
pH 7.4, 30°C, wild-type ferredoxin from the cyanobacterium Anabaena PCC 7119 |
696462 |
| 1.14.15.8 | 0.00734 |
- |
reduced flavodoxin |
pH 7.4, 30°C, wild-type flavodoxin from the cyanobacterium Anabaena PCC 7119 |
696462 |
| 1.14.15.8 | 0.0007 |
- |
progesterone |
pH 7.4, 30°C, mutant K27R/I71T/I215T |
697323 |
