Cloned (Comment) | Organism |
---|---|
CYP106A2 can be easily expressed in Escherichia coli with a high yield and can be reconstituted using the adrenal redox proteins, adrenodoxin and adrenodoxin reductase | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
A106T | kcat/Km for conversion of 11-deoxycortisol is 3.17fold higher than wild-type value, 1.43fold increase in progesterone conversion | Priestia megaterium |
A106T/Q189K/T399S/R409L | kcat/Km for conversion of 11-deoxycortisol is 4.3fold higher than wild-type value, 1.13fold increase in progesterone conversion | Priestia megaterium |
A106T/R409L | kcat/Km for conversion of 11-deoxycortisol is 3fold higher than wild-type value, 1.43fold increase in progesterone conversion | Priestia megaterium |
D153V/I214F | kcat/Km for conversion of 11-deoxycortisol is 2.64fold higher than wild-type value, progesterone conversion is 93% of wild-type value | Priestia megaterium |
D217V | kcat/Km for conversion of 11-deoxycortisol is 2.68fold higher than wild-type value, 1.18fold increase in progesterone conversion | Priestia megaterium |
F165I | 1.25fold increase in progesterone conversion | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant A106T/Q189K/T399S/R409L, mutant D153V/I214F | Priestia megaterium | |
0.059 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant A106T | Priestia megaterium | |
0.066 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant D217V | Priestia megaterium | |
0.068 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant A106T/R409L | Priestia megaterium | |
0.093 | - |
11-deoxycortisol | pH 7.4, 30°C, CYP106A2 wild-type enzyme | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | Q06069 | ATCC 13368 | - |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycortisol + reduced adrenodoxin + O2 | adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland | Priestia megaterium | 15beta-15,17,21-trihydroxypregn-4-ene-3,20-dione + oxidized adrenodoxin + H2O | - |
? | |
progesterone + reduced adrenodoxin + O2 | adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland | Priestia megaterium | 15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP106A2 | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Priestia megaterium | |
NADPH | - |
Priestia megaterium |