Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.251 | - |
progesterone | pH 7.4, 30°C, for determination of Km-value the formation of the product 15beta-hydroxy-progesterone is determined | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | Q06069 | ATCC 13368, recombinantly expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the Vmax values for 15beta-hydroxyprogesterone, 6beta-hydroxyprogesterone, 11alpha-hydroxyprogesterone, and 9alpha-hydroxyprogesterone are determined as 337.3, 22.3, 17.5, and 6.5 nmol product/min/nmol CYP106A2 | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + reduced adrenodoxin + O2 | identification of monohydroxy progesterones using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry. 15beta-Hydroxy-progesterone is the main product. 6beta-Hydroxyprogesterone, 11alpha-hydroxyprogesterone, and 9alpha-hydroxyprogesterone are formed at small amounts | Priestia megaterium | 15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP106A2 | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Priestia megaterium |