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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
1.14.15.8
0.06
-
deoxycorticosterone
22°C
698682
1.14.15.8
0.095
-
corticosterone
22°C
698682
1.14.15.8
0.143
-
progesterone
22°C
698682
1.14.15.8
0.45
-
20alpha-dihydroprogesterone
22°C
698682
1.14.15.8
0.56
-
17alpha-hydroxyprogesterone
22°C
698682
1.14.15.8
0.625
-
4-androstene-3,17-dione
22°C
698682
1.14.15.8
1.11
-
testosterone
22°C
698682
1.14.15.8
-999
-
more
KM-value for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2
696462
1.14.15.8
0.093
-
11-deoxycortisol
pH 7.4, 30°C, CYP106A2 wild-type enzyme
693956
1.14.15.8
0.251
-
progesterone
pH 7.4, 30°C, for determination of Km-value the formation of the product 15beta-hydroxy-progesterone is determined
695981
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