EC Number   |
General Stability |
Reference |
|---|
   3.2.1.106 | 1% poly(ethylene glycol) 4000 stabilizes |
170969 |
| 3.2.1.106 | bovine serum albumin, 1-8 mg/ml, enhances stability during assay |
170949 |
| 3.2.1.106 | native form of glucosidase I is unusually resistant to non-specific proteolysis, partial digestion with trypsin requires rather high concentrations of the protease, at high, that is equimolar, concentrations of trypsin enzyme is cleaved into a defined and enzymatically active mixture of protein fragments with molecular mass of 69 kDa, 45 kDa and 29 kDa |
170971 |
| 3.2.1.106 | quite stable at all stages of purification |
170949 |
| 3.2.1.106 | resistant to trypsin or subtilisin in sealed microsomal vesicles, in unsealed vesicles by the addition of 2 mg/ml Triton X-100, inactivation within 5 min |
170958 |
| 3.2.1.106 | stable to lyophilization without dialysis after step 3 of purification |
170949 |
| 3.2.1.106 | trypsin hydrolysis releases polypeptides containing the alpha-glucosidase I catalytic domain, with no loss of catalytic activity |
657267 |
| 3.2.1.106 | unstable to dialysis against, 10 mM potassium phosphate buffer, pH 6.8, containing 0.002% Hibitane, using either dialysis bags or diafiltration |
170949 |
