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Literature summary for 3.2.1.106 extracted from
- Purification and characterization of glucosidase I involved in N-linked glycoprotein processing in bovine mammary gland (1987), Biochem. J., 247, 555-562.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Lubrol Px | activation | Rattus norvegicus |  |
| Lubrol Px | activation | Bos taurus | |
| additional information | no significant activation by phospholipids | Bos taurus | |
| Triton X-100 | - |
Rattus norvegicus | |
| Triton X-100 | - |
Bos taurus | |
General Stability
| General Stability | Organism |
|---|---|
| 1% poly(ethylene glycol) 4000 stabilizes | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxynojirimycin | strong inhibition | Bos taurus | |
| 1-deoxynojirimycin | - |
Mus musculus | |
| 1-deoxynojirimycin | - |
Rattus norvegicus | |
| Ag+ | potent inhibitor, inhibition reversed by adding an excess of dithiothreitol | Bos taurus | |
| Co2+ | potent inhibitor, inhibition reversed by adding an excess of dithiothreitol | Bos taurus | |
| Cu2+ | potent inhibitor, inhibition reversed by adding an excess of dithiothreitol | Bos taurus | |
| D-glucose | no significant inhibition, 10 mM: 13% inhibition | Bos taurus | |
| D-mannose | 10 mM: no inhibition | Bos taurus | |
| heavy-metal ions | potent inhibitors, inhibition reversed by adding an excess of dithiothreitol | Bos taurus | |
| Hg2+ | potent inhibitor, inhibition reversed by adding an excess of dithiothreitol | Bos taurus | |
| kojibiose | 1 mM: 76% inhibition; alpha-1,2-linked glucose disaccharide | Bos taurus | |
| additional information | no inhibition by alpha,alpha'-bipyridyl and o-phenanthroline; no inhibition by EDTA; no significant inhibition by nigerose, Glc(alpha1-3)Glc disaccharide, maltose, gentibiose, cellobiose, sucrose | Bos taurus | |
| additional information | - |
Mus musculus | |
| additional information | no inhibition by EDTA | Rattus norvegicus | |
| N-(5-carboxypentyl)-1-deoxynojirimycin | - |
Bos taurus | |
| p-nitrophenyl-alpha-D-glucoside | 0.0005 mM: 19% inhibition | Bos taurus | |
| p-nitrophenyl-alpha-D-glucoside | - |
Rattus norvegicus | |
| p-nitrophenyl-beta-D-glucoside | 0.0005 mM: 14% inhibition | Bos taurus | |
| p-nitrophenyl-beta-D-glucoside | - |
Rattus norvegicus | |
| Zn2+ | potent inhibitor, inhibition reversed by adding an excess of dithiothreitol | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| endoplasmic reticulum | - |
Bos taurus | 5783 | - |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Bos taurus | - |
- |
| additional information | - |
Rattus norvegicus | - |
- |
| additional information | specific antibodies against glucosidase I allow subcellular localization of glucosidase I | Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Rattus norvegicus | |
| additional information | metal ions not required | Bos taurus | |
| phosphate | activation up to 25 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
4 * 85000, SDS-PAGE reducing conditions | Bos taurus |
| 320000 | 330000 | gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc3Man9GlcNAc2 + H2O | Cavia porcellus | - |
? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | Rattus norvegicus | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | Bos taurus | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | Rattus norvegicus | specific involvement in the N-linked oligosaccharide-processing pathway | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | Bos taurus | specific involvement in the N-linked oligosaccharide-processing pathway | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | Mus musculus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | Rattus norvegicus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | Bos taurus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
| additional information | Mus musculus | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | ? | - |
? | |
| additional information | Rattus norvegicus | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | ? | - |
? | |
| additional information | Bos taurus | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | ? | - |
? | |
| additional information | Mus musculus | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
| additional information | Rattus norvegicus | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
| additional information | Bos taurus | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
| additional information | Mus musculus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
| additional information | Rattus norvegicus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
| additional information | Bos taurus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
| additional information | Bos taurus | glucosidase I is a control enzyme for the regulation of glycoprotein processing | ? | - |
? | |
| additional information | Bos taurus | play a crucial role in the overall regulation of glycoprotein biosynthesis | ? | - |
? | |
| additional information | Mus musculus | involved in the formation of high mannose and complex glycoproteins | ? | - |
? | |
| additional information | Bos taurus | involved in the formation of high mannose and complex glycoproteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
cow | - |
| Bos taurus | - |
lactating cow | - |
| Cavia porcellus | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| lactating mammary gland | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Cavia porcellus | - |
| heart | - |
Bos taurus | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| mammary gland | - |
Cavia porcellus | - |
| mammary gland | - |
Mus musculus | - |
| mammary gland | - |
Rattus norvegicus | - |
| mammary gland | lactating mammary glands | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months, without loss of activity | Bos taurus |
| 25°C, buffer containing 1% poly(ethylene glycol) 4000, 72 h, 75% activity retained | Bos taurus |
| 4°C, 1 week, without loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc3Man9GlcNAc2 + H2O | - |
Cavia porcellus | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
| Glc3Man9GlcNAc2 + H2O | - |
Mus musculus | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
| Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Rattus norvegicus | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
| Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Bos taurus | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
| Glc3Man9GlcNAc2 + H2O | - |
Cavia porcellus | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Rattus norvegicus | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Bos taurus | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | specific involvement in the N-linked oligosaccharide-processing pathway | Rattus norvegicus | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | specific involvement in the N-linked oligosaccharide-processing pathway | Bos taurus | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | involved in processing of asparagine-linked oligosaccharides | Mus musculus | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | involved in processing of asparagine-linked oligosaccharides | Rattus norvegicus | ? | - |
? | |
| Glc3Man9GlcNAc2 + H2O | involved in processing of asparagine-linked oligosaccharides | Bos taurus | ? | - |
? | |
| additional information | Glc1Man9GlcNAc2: not a substrate | Bos taurus | ? | - |
? | |
| additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Rattus norvegicus | ? | - |
? | |
| additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Bos taurus | ? | - |
? | |
| additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Bos taurus | ? | - |
? | |
| additional information | Glc2Man9GlcNAc2: not a substrate | Bos taurus | ? | - |
? | |
| additional information | p-nitrophenyl-beta-D-glucopyranoside: not a substrate | Bos taurus | ? | - |
? | |
| additional information | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | Mus musculus | ? | - |
? | |
| additional information | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | Rattus norvegicus | ? | - |
? | |
| additional information | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | Bos taurus | ? | - |
? | |
| additional information | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Mus musculus | ? | - |
? | |
| additional information | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Rattus norvegicus | ? | - |
? | |
| additional information | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Bos taurus | ? | - |
? | |
| additional information | involved in processing of asparagine-linked oligosaccharides | Mus musculus | ? | - |
? | |
| additional information | involved in processing of asparagine-linked oligosaccharides | Rattus norvegicus | ? | - |
? | |
| additional information | involved in processing of asparagine-linked oligosaccharides | Bos taurus | ? | - |
? | |
| additional information | glucosidase I is a control enzyme for the regulation of glycoprotein processing | Bos taurus | ? | - |
? | |
| additional information | play a crucial role in the overall regulation of glycoprotein biosynthesis | Bos taurus | ? | - |
? | |
| additional information | involved in the formation of high mannose and complex glycoproteins | Mus musculus | ? | - |
? | |
| additional information | involved in the formation of high mannose and complex glycoproteins | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 85000, SDS-PAGE reducing conditions | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Rattus norvegicus |
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
50% activity lost after 24 h, in buffer containing 1% poly(ethylene glycol) 4000: 75% activity retained after 72 h | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | half-maximal activity at | Bos taurus |
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