Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | about 45% activation of membrane-bound activity, maximum stimulation of membrane-bound activity at 0.5% Triton X-100, no activation of soluble activity | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
bovine serum albumin, 1-8 mg/ml, enhances stability during assay | Saccharomyces cerevisiae |
quite stable at all stages of purification | Saccharomyces cerevisiae |
stable to lyophilization without dialysis after step 3 of purification | Saccharomyces cerevisiae |
unstable to dialysis against, 10 mM potassium phosphate buffer, pH 6.8, containing 0.002% Hibitane, using either dialysis bags or diafiltration | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by dithiothreitol, 2-mercaptoethanol, Mg2+, Mn2+, EDTA, potassium phosphate | Saccharomyces cerevisiae | |
octyl-beta-glucoside | 10 mM: 90% inhibition | Saccharomyces cerevisiae | |
p-Aminophenyl-beta-thioglucoside | 100 mM: 73% inhibition | Saccharomyces cerevisiae | |
Tris/maleate | 10 mM: 60% inhibition | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | X-2180, equally distributed between particulate and supernatant fractions | Saccharomyces cerevisiae | 16020 | - |
additional information | X-2180, equally distributed between particulate and supernatant fractions | Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | specific involvement in the N-linked oligosaccharide-processing pathway | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
additional information | Saccharomyces cerevisiae | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
additional information | Saccharomyces cerevisiae | involved in the formation of high mannose and complex glycoproteins | ? | - |
? | |
additional information | Saccharomyces cerevisiae X-2180 | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
X-2180 | - |
Saccharomyces cerevisiae X-2180 | - |
X-2180 | - |
Purification (Comment) | Organism |
---|---|
partial, soluble glucosidase I | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
2.6 | - |
soluble activity | Saccharomyces cerevisiae |
5.5 | - |
membrane-bound activity, with Triton X-100 | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-26°C, lyophilized, stable for at least 1 month | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Saccharomyces cerevisiae | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Saccharomyces cerevisiae | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | specific involvement in the N-linked oligosaccharide-processing pathway | Saccharomyces cerevisiae | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Glc1Man9GlcNAc2: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Saccharomyces cerevisiae | ? | - |
? | |
additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | very slightly active with Glc2Man9GlcNAc2 | Saccharomyces cerevisiae | ? | - |
? | |
additional information | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | involved in the formation of high mannose and complex glycoproteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Glc1Man9GlcNAc2: not a substrate | Saccharomyces cerevisiae X-2180 | ? | - |
? | |
additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Saccharomyces cerevisiae X-2180 | ? | - |
? | |
additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Saccharomyces cerevisiae X-2180 | ? | - |
? | |
additional information | very slightly active with Glc2Man9GlcNAc2 | Saccharomyces cerevisiae X-2180 | ? | - |
? | |
additional information | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Saccharomyces cerevisiae X-2180 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
rapid decrease in activity on acidic side | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: about 35% of maximal activity, pH 8.5: about 15% of maximal activity, pH 5.8: no activity | Saccharomyces cerevisiae |